A novel approach to treat acute respiratory failure by combination of type 2 alveolar epithelial regeneration and alveolar macrophage inhibition

• Project/Area Number: 21591810
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Niigata University
• Principal Investigator: TSUCHIDA Masanori 新潟大学, 医歯学系, 教授 (60293221)
• Co-Investigator(Kenkyū-buntansha): SHINOHARA Hirohiko 新潟大学, 医歯学系, 助教
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 肺障害 / 気道上皮再生 / サイトカイン / 線維化 / 培養細胞 / 肺胞上皮 / 遺伝子導入 / HGF

Research Abstract

In vitro cell culture experiment demonstrated that airway epithelial cell growth was improved by adding cytokines in the culture medium. Erectroporation also improved efficacy of gene introduction into culture cells of airway epithelium. Gene expression was observed in the alveolar epithelium by intratracheal administration of naked gene. However, lung injury due to pressure to epithelium was observed.