| Project/Area Number |
21591812
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKAMURA Meinoshin 大阪大学, 医学系研究科, 教授 (40252647)
MINAMI Masato 大阪大学, 医学部附属病院, 准教授 (10240847)
SAWABATA Noriyoshi 大阪大学, 医学系研究科, 准教授 (50403184)
KADOTA Ybshihisa 大阪大学, 医学系研究科, 助教 (50464243)
内海 朝喜 大阪大学, 医学系研究科, 助教 (40423165)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 呼吸器外科学 / 肺癌 / Dormant Cancer Cell / 微小転移 / 再発制御 / 抗癌剤感受性 / One-step nucleic acid amplification法 |
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| Research Abstract |
We have investigated the usefulness of one-step nucleic acid amplification(OSNA) method to detect lymph node metastasis with exploration of target markers. Molecules with high expression in lung cancer were extracted using a gene data base and the expression in the lymph nodes was analyzed with OSNA method. We found the significant expression of KRT19 and KRT7 in metastatic lymph nodes using OSNA method. One false negative was found due to massive necrosis of cancer cells and one false positive was caused by the non-specific cytokeratin expression of epithelioid cells in the lymph node with OSNA assay using CK19. In conclusion, OSNA assay using CK19 could be available for the rapid molecular diagnosis of lymph node metastasis in lung cancer.
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