Establishment of rapid Intraoperative Detection of Lymph Node metastasis Using Molecularbiological Method

• Project/Area Number: 21591818
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kumamoto University
• Principal Investigator: SHIRAISHI Kenji 熊本大学, 医学部附属病院, 非常勤診療医師 (70363538)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: TRC法 / リンパ節転移 / 肺癌

Research Abstract

To improve the results of surgical treatment for lung cancer, a molecular biological TRC method(Transcription Reverse transcription Concerted reaction) was carried out to quantify the messenger RNA level of CEA. This method have high sensitivity in detecting the presence of cancer cells, but more investigation need to verify the accuracy of this method, for exsample, the matching of the result with histopathological diagnosis.