Detection of tyrosine kinase gene mutation using genotyping assay

• Project/Area Number: 21591820
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Nagoya City University
• Principal Investigator: SASAKI Hidefumi 名古屋市立大学, 大学院・医学研究科, 准教授 (00336695)
• Co-Investigator(Kenkyū-buntansha): YANO Motoki 名古屋市立大学, 大学院・医学研究科, 准教授 (40315883)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 肺癌 / KIF5B/RET / FISH / EGFR / Kras / 非喫煙者 / 遺伝子転座 / 腺癌 / タバコ / NRF2 / NFE2L2 / 扁平上皮癌 / 分子標的 / 遺伝子増幅 / ジェノタイピング

Research Abstract

Novel KIF5B/RET translocation was found in lung cancer using a next-generation sequencing assay. We have developed fluorescence in situ hybridization(FISH) assay to detect KIF5B/RET transfusion gene. We have investigated Kras gene mutation statuses using genotyping assay and compared with Kras copy number, Glut-1 expression and other clinico-pathological backgrounds of lung cancers.

Research Products

• [Journal Article] Identification of new ALK and RET gene fusions from colorectal and lung cancer biopsies (2012)
  • Author(s): Lipson D, Capelletti M, Yelensky R, Sasaki H(24番目), et al
  • Journal Title: Nat Med Volume: 18 Pages: 382-384
  • Peer Reviewed
• [Journal Article] Overexpression of GLUT1 correlates with Kras mutations in lung carcinomas (2012)
  • Author(s): Sasaki H, Shitara M, Yokota K, Hikosaka Y, Moriyama S, Yano M, Fujii Y
  • Journal Title: Mol Med Report Volume: 5 Pages: 599-602
  • Peer Reviewed
• [Journal Article] Identification of new ALK and RET fusions from colorectal and lung cancer biopsies (2012)
  • Author(s): Lipson D, et al
  • Journal Title: Nat Med Volume: 18 Issue: 3 Pages: 382-384
  • DOI: 10.1038/nm.2673
  • Peer Reviewed
• [Journal Article] Evaluation of Kras gene mutation and copy number gain in non-small cell lung cancer (2011)
  • Author(s): Sasaki H, Hikosaka Y, Kawano O, Moriyama S, Yano M, Fujii Y
  • Journal Title: J Thorac Oncol Volume: 6 Pages: 15-20
  • Peer Reviewed
• [Journal Article] LKB1 gene alterations in surgically resectable adenocarcinoma of the lung (2011)
  • Author(s): Okuda K, Sasaki H, Hikosaka Y, Kawano O, Moriyama S, Yano M, Fujii Y
  • Journal Title: Surg Today Volume: 41 Pages: 107-110
  • NAID: 10027818817
  • Peer Reviewed
• [Journal Article] Long exposure of environmental tobacco smoke associated with activating EGFR mutations in never-smokers with non-small cell lung cancer (2011)
  • Author(s): Kawaguchi T, Ando M, Kubo A, Takada M, Atagi S, Okishio K, Asami K, Matsumura A, Tsujino K, Ignatius OS, Sasaki H
  • Journal Title: Clin Cancer Res Volume: 17 Pages: 39-45
  • Peer Reviewed
• [Journal Article] Meta and pooled analyses of FGFR4 Gly388Arg polymorphism as a cancer prognostic factor (2011)
  • Author(s): Frullanti E, Berking C, Harbeck N, jezequel P, Haugen A, Mawrin C, Parise O Jr, Sasaki H, Tsuchiya N, Dragani TA
  • Journal Title: Eur J Cancer Prev Volume: 20 Pages: 340-347
  • Peer Reviewed
• [Journal Article] Evaluation of Kras gene mutation and copy number gain in non-small cell lung cancer. (2011)
  • Author(s): Kawaguchi T, Sasaki H, (11番目), et al.
  • Journal Title: Clin Cancer Res Volume: 17 Pages: 39-45
  • Peer Reviewed
• [Journal Article] CHRNA5 gene D3998N polymorphism in Japanese lung adenocarcinoma (2010)
  • Author(s): Sasaki H, Hikosaka Y, Okuda K, Kawano O, Yukiue H, Yano M, Fujii Y
  • Journal Title: J Surg Res Volume: 162 Pages: 75-78
  • Peer Reviewed
• [Journal Article] The landscape of somatic copy-number alteration across human cancers (2010)
  • Author(s): Beroukhim R, Mermel CH, Porter D, Sasaki H(33番目), et al
  • Journal Title: Nature Volume: 463 Pages: 899-905
  • Peer Reviewed
• [Journal Article] Evaluation of Kras gene mutation and copy number in thymic carcinomas and thymomas (2010)
  • Author(s): Sasaki H, Yano M, Fujii Y
  • Journal Title: J Thorac Oncol Volume: 5 Pages: 1715-1716
  • Peer Reviewed
• [Journal Article] CHRNA5 gene D398N polymorphism in Japanese lung adenocarcinoma (2010)
  • Author(s): Sasaki H, et al.
  • Journal Title: J Surg Res Volume: 162(1) Pages: 75-78
  • Peer Reviewed
• [Journal Article] Evaluation of Kras gene mutation and copy number in thymic carcinomas and thymomas. (2010)
  • Author(s): Sasaki H, et al.
  • Journal Title: J Thorac Oncol Volume: 5(10) Pages: 1715-1716
  • Peer Reviewed
• [Journal Article] Epidermal growth factor receptor gene amplification in surgical resected Japanese lung cancer (2009)
  • Author(s): Sasaki H, Shimizu S, Okuda K, Kawano O, Yukiue H, Yano M, Fujii Y
  • Journal Title: Lung Cancer Volume: 64 Pages: 295-300
  • Peer Reviewed
• [Journal Article] Epidermal growth factor receptor gene amplification in surgical resected non-small cell lung cancer (2009)
  • Author(s): 佐々木秀文, 他
  • Journal Title: Lung Cancer 64(3) Pages: 295-300
  • Peer Reviewed
• [Journal Article] Thymoma with dissemination : efficacy of microscopic total resection of disseminated nodules (2009)
  • Author(s): 矢野智紀, 佐々木秀文, 他
  • Journal Title: World J Surg 33(7) Pages: 1425-1431
  • Peer Reviewed
• [Journal Article] Gefitinib が無効でS-1が著効したEGFR遺伝子変異陽性肺癌の1例 (2009)
  • Author(s): 川野理, 矢野智紀, 佐々木秀文, 他
  • Journal Title: 癌と化学療法 36(4) Pages: 675-677
• [Presentation] 肺癌におけるKras遺伝子変異およびコピー数の増加に関する検討 (2011)
  • Author(s): 佐々木秀文
  • Organizer: 第52回日本肺癌学会総会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2011-11-04
• [Presentation] 肺癌におけるKras遺伝子変異およびコピー数の増加に関する検討 (2011)
  • Author(s): 佐々木秀文、設楽将之、横田圭右、奥田勝裕、彦坂雄、森山悟、矢野智紀、藤井義敬
  • Organizer: 第52回日本肺癌学会総会
  • Place of Presentation: 大阪
• [Presentation] 肺癌におけるKras遺伝子変異および増幅に関する検討 (2011)
  • Author(s): 佐々木秀文、設楽将之、横田圭右、奥田勝裕、彦坂雄、森山悟、矢野智紀、藤井義敬
  • Organizer: 第64回日本胸部外科学会定期学術集会
  • Place of Presentation: 名古屋
• [Presentation] 非小細胞肺癌におけるKras遺伝子変異とコピー数増加に関する検討 (2011)
  • Author(s): 佐々木秀文、奥田勝裕、彦坂雄、森山悟、矢野智紀、藤井義敬
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Evaluation of Kras gene mutation and copy number in non-small cell lung cancer (2011)
  • Author(s): Sasaki H, Okuda K, Hikosaka Y, Moriyama S, Yano M, Fujii Y
  • Organizer: 14th world conference on lung cancer
  • Place of Presentation: Amsterdam
• [Presentation] 胸腺腫瘍におけるKras遺伝子変異および遺伝子コピー数に関する検討 (2011)
  • Author(s): 佐々木秀文、横田圭右、奥田勝裕、彦坂雄、森山悟、矢野智紀、藤井義敬
  • Organizer: 第111回日本外科学会定期学術集会
  • Place of Presentation: 東京
• [Presentation] 日本人肺扁平上皮癌におけるNRF2遺伝子変異は予後と相関する (2010)
  • Author(s): 佐々木秀文
  • Organizer: 第63回日本胸部外科学会定期学術総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-10-27
• [Presentation] Association of gender and passive smoking with epidermal growth factor receptor mutations in never-smokers with non-small cell lung cancer : A prospective study based on detailed passive smoking questionnarire (2010)
  • Author(s): Kawaguchi T, Ando M, Kubo A, Takada M, Atagi S, Okishio K, Asami K, Matsumura A, Tsujino K, Sasaki H
  • Organizer: 2010 ASCO annual meeting, central poster session
  • Place of Presentation: Chicago
• [Presentation] 日本人肺腺癌患者におけるMEK1およびAKT2遺伝子変異の検討 (2009)
  • Author(s): 佐々木秀文, 他
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-10-03
• [Presentation] Epidermal growth factor receptor gene amplification in surgical resected Japanese lung cancer (2009)
  • Author(s): Sasaki H, Okuda K, Hikosaka Y, Kawano O, Yano M, Fujii Y, Takada M, Kawaguchi T, Kawahara M
  • Organizer: 13th world conference on lung cancer
  • Place of Presentation: San Francisco
• [Patent(Industrial Property Rights)] KIF5B遺伝子とRET遺伝子との間の転座を検出するFISHアッセイ (2012)
  • Inventor(s): 佐々木秀文
  • Industrial Property Rights Holder: 名古屋市立大学、ダコ・ジャパン株式会社、株式会社GSP研究所
  • Industrial Property Number: 2012-015939
  • Filing Date: 2012-01-27