Development of a novel sealant material for lung air leaks by the cell sheet engineering
| Project/Area Number |
21591827
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
KANZAKI Masato 東京女子医科大学, 医学部, 講師 (80277136)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 呼吸器外科 / 肺気漏 / 細胞シート / 組織工学 / 組織修復材 / 気漏閉鎖 / 肺切除 / 術中肺気漏 / 組織修復接着剤 / 人工補強材 / 細胞外マトリックス / 胸膜癒着術 |
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| Research Abstract |
After 1 week, skin fibroblasts cultured on the temperature-responsive surfaces were then transferred at 20℃for non-invasive cell sheet harvest. For cell sheet transplantation, F-344 athymic rats were performed a left-lateral thoracotomy. An incision was set in left lung. Air leakages were confirmed and closed with the transplantation of cell sheets. After 4 weeks, re-thoracotomy was performed and the transplanted area was observed. Fibroblast sheets adhered to the lung surface without leural adhesion. Neovascular structure was present around the transplanted fibroblast sheet. On the outer surface, host mesothelial cells had migrated to cover the transplanted fibroblast sheet. In transmission electron microscopy, mesothelial cells had short immature microvilli and transplanted fibroblast sheets had present abundant extracellular matrix. Fibroblast sheets had secreted basic-FGF, VEGF, and HGF.
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Report
(4 results)
Research Products
(36 results)
