| Project/Area Number |
21591831
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIGEMATSU Yoshiki 産業医科大学, 医学部, 助教 (10546469)
YASUMOTO Kosei 産業医科大学, 医学部, 名誉教授 (30150452)
ONO Kenji 産業医科大学, 医学部, 助教 (40369062)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | EGFR / lung cancer / heterogeneity |
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| Research Abstract |
Lung cancer has been the most common cancer in the world for several decades and the prognosis is currently unacceptable. Activating mutations in the epidermal growth factor receptor(EGFR) gene in lung adenocarcinoma are associated with a dramatic clinical response to EGFR-tyrosine kinase inhibitors(TKI). However, patients often experience a relapse. However, the whole picture and biological significance regarding acquired resistance remains unclear. in this research. We planned the experiment to establish the visualization for the cancer cell transferred for GFR gene transiently and stably by using lung cancer cell lines, measurement for tumor growth potential by co-culture, and investigation of relationship between mix ratio and drug resistance to EGFR-TKI. We also examined the clinical feature of adenosquamous cell carcinoma as a representative model for heterogeneity of the tumor. Furtherer, our results showed that various alterations in gene or protein expression can account for all resistant mechanisms and this phenomenon suggests the existence of complicated relationships among acquired resistance-related genes. We also reported that T790M may thus be a useful marker for predicting a favorable prognosis in Japanese patients treated by an EGFR-TKI.
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