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Analysis of CD133 positive cells in pituitary adenoma

Research Project

Project/Area Number 21591875
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionKagoshima University

Principal Investigator

ARITA Kazunori  鹿児島大学, 医歯学総合研究科, 教授 (90212646)

Co-Investigator(Kenkyū-buntansha) YUNOUE Syunji  鹿児島大学, 医学部・歯学部附属病院, 助教 (20404478)
HIRANO Hirofumi  鹿児島大学, 医学部・歯学部附属病院, 講師 (00264416)
TAKANO Kouji  東京大学, 大学病院, 助教 (20236243)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordspituitary adenoma / CD133 / endothelial progenitor / angiogenesis / tumor stem cell / 下垂体腺腫 / 腫瘍幹細胞 / CD133陽性細胞 / endothelial progenitor cell
Research Abstract

Stem-like cells in tumors are capable of self-renewal and pluri-differentiation ; they are thought to play important roles in tumor initiation and maintenance. Stem-like cells in malignant glioma express CD133. We examined samples from human pituitary adenoma, a generally benign neoplasm, for CD133 expression using routine immunohistochemical and biochemical methods.
Our study of 70 pituitary adenomas(clinically nonfunctioning adenomas and growth hormone-, prolactin-, adrenocorticotropic hormone-, and thyroid stimulating hormone producing adenomas) showed that 18(25. 7%) expressed CD133. This rate was higher in clinically non-functioning(33.3%) than functioning adenomas(12.0%)(p=0.085). Real-time PCR assay revealed the expression of CD133 mRNA in samples immunohistochemically positive for CD133. Neither the patient age and gender, nor the tumor size or postoperative recurrence rate correlated with CD133 positivity. CD133^+cells ubiquitously coexpressed CD34, nestin, and VEGFR2(KDL1). S-100 and GFAP were not coexpressed with CD133. Chromogranin A, Pit-1, SF-1, and NeuroD1 were immune-negative, indicating that CD133^+cells did not have the potential to differentiate into functional endocrine cells.
Our data suggest that the expression of CD133 in pituitary adenomas is related to immature endothelial progenitor cells that may play a role in the neovascularization of pituitary adenomas. Further studies are needed to elucidate the significance of CD133^+ cells with respect to neovascularization and their sustainable growth in pituitary adenomas.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (6 results)

All 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Identi fication of CD133+cells in pituitary adenomas2011

    • Author(s)
      Yunoue S, Arita K, Kawano H, Uchida H, Tokimura H, Hirano H.
    • Journal Title

      Neuroendocrinology

      Volume: 94(4) Pages: 302-12

    • Related Report
      2011 Final Research Report
  • [Journal Article] Identification of CD 133+ Cells in Pituitary Adenomas2011

    • Author(s)
      Yunoue S, Arita K, Kawano H, Uchida H, Tokimura H, Hirano H
    • Journal Title

      Neuroendocrinology

      Volume: 94 Issue: 4 Pages: 302-312

    • DOI

      10.1159/000330625

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 下垂体腺腫におけるCD133陽性細胞~その同定と存在意義2011

    • Author(s)
      湯之上俊二、藤尾信吾、羽生未佳、ユーリズバクティアル、平野宏文、時村洋、有田和徳
    • Organizer
      第15回内分泌病理学会
    • Place of Presentation
      都道府県会館(東京)
    • Year and Date
      2011-11-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 下垂体腺腫におけるCD133陽性細胞~その同定と存在意義2011

    • Author(s)
      湯之上俊二
    • Organizer
      第15回日本内分泌病理学会学術集会
    • Place of Presentation
      都道府県会館(東京都)
    • Year and Date
      2011-11-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 超高齢者における下垂体腺腫の手術療法2010

    • Author(s)
      湯之上俊二
    • Organizer
      社団法人日本脳神経外科学会第69回学術総会
    • Place of Presentation
      福岡コンベンションセンター(福岡県福岡市)
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 下垂体腺腫おける endothelial progenitor cell2010

    • Author(s)
      湯之上俊二, 川野弘人, 内田裕之, 時村洋, 平野宏文, 有田和徳
    • Organizer
      第83回内分泌学会学術総会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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