Transduction of neurogenesis inducing gene to the hippocampus
| Project/Area Number |
21591882
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Madoka 順天堂大学, 医学部, 助教 (50317450)
ARAI Hjime 順天堂大学, 医学部, 教授 (70167229)
NISHIMURA Kinya 順天堂大学, 医学部, 教授 (80164581)
望月 秀樹 順天堂大学, 医学部, 准教授 (90230044)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 機能脳神経外科学 / 海馬 / 神経細胞新生 / てんかん / パッチクランプ / VEGF |
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| Research Abstract |
【Purpose】Purpose is inducing maturation of newborn neurons by transduction of PEDF and VEGF.
【Methods】We transduce PEDF and VEGF using retrovirus vector to epilepsy mice. We observe the maturation of newborn neurons by immuno-histology and patch-clamp technique.
【Observation results】Many newborn neurons were immature that were characterized as follows ; negative for NeuN, high input resistance and -55 mV to -70 mV of resting membrane potentials. Transduction of PEDF and VEGF did not induce the maturation of newborn neurons.
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Report
(4 results)
Research Products
(21 results)
