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Development of new drugs for glioma stem cells in using human neural stem cells as in vitro model cells for drug screening and toxicity studies

Research Project

Project/Area Number 21591888
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionNational Hospital Organization Osaka National Hospital Institute for Clinical Reserch

Principal Investigator

KANEMURA Yonehiro  独立行政法人国立病院機構大阪医療センター(臨床研究センター), 先進医療研究開発部再生医療研究室, 室長 (80344175)

Co-Investigator(Kenkyū-buntansha) SHOFUDA Tomoko  独立行政法人国立病院機構大阪医療センター(臨床研究センター), 先進医療研究開発部・幹細胞医療研究室, 室長 (40450895)
Co-Investigator(Renkei-kenkyūsha) MORIUCHI Shusuke  りんくう総合医療センター, 脳神経外科, 部長 (90322180)
NAITO Takeaki  神戸薬科大学, 薬学部, 教授 (00068339)
MIYATA Okiko  神戸薬科大学, 薬学部, 教授 (90102110)
ARITA Norio  兵庫医科大学, 医学部, 教授 (80159508)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords脳腫瘍学 / グリオーマ幹細胞 / 脳神経疾患 / 薬剤反応性 / 癌
Research Abstract

In this research, to develop new drugs for malignant gliomas(MGs), we examined the in vitro chemosensitivity of glioma stem cells(GSCs) compared with normal neural stem cells(NSCs), which we used in vitro model cells for drug screening and toxicity studies. We isolated three GSC lines isolated from surgically removed MG tissues and examined their detailed in vitro cellular properties. In vitro chemosensitivity to drugs for MGs(ACNU, TMZ, INF-β, VCR, PCZ) or other compounds against GSCs and NSCs were examined. We found that both GSCs and NSCs showed similar chemosensitivities and several compounds showed significant cytotoxic effect to GCSs and NSCs equally. These findings will increase our knowledge about the cellular properties of GSCs and provide useful information for the development of new drugs that target GSCs.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (43 results)

All 2012 2011 2010 2009

All Journal Article (11 results) (of which Peer Reviewed: 6 results) Presentation (30 results) Book (2 results)

  • [Journal Article] Methamphetamine induces endoplasmic reticulum stress related gene CHOP/Gadd153/ddit3 in dopaminergic cells2011

    • Author(s)
      Irie, Yasuyuki; Saeki, Makio; Tanaka, Hidekazu; Kanemura, Yonehiro; Otake, Shinpei; Ozono, Yoshiyuki; Nagai, Toshisaburou; Kondo, Yukiko; Kudo, Kenzo; Kamisaki, Yoshinori; Miki, Naomasa; Taira, Eiichi
    • Journal Title

      Cell and Tissue Research

      Volume: Vol.345:2 Issue: 2 Pages: 231-241

    • DOI

      10.1007/s00441-011-1207-5

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 中枢神経疾患に対する細胞移植療法を用いた再生治療2011

    • Author(s)
      金村米博
    • Journal Title

      脳神経外科

      Volume: 39(1) Pages: 5-23

    • URL

      http://www.bitway.ne.jp/ejournal/club/1436101316.html

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] 自己腫瘍ライセートをエレクトロポレーション法で取り込ませた樹状細胞はIFN-γ産生細胞の誘導を高める2010

    • Author(s)
      高原将司,金村米博,贄田美江,前川隆司,森内秀祐
    • Journal Title

      Biotherapy

      Volume: 24(1) Pages: 21-26

    • URL

      http://www.pieronline.jp/content/article/0914-2223/24010/21

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 神経幹細胞樹立に使用可能な細胞ソースと細胞品質管理2010

    • Author(s)
      金村米博
    • Journal Title

      実験医学増刊

      Volume: 28(2) Pages: 75-82

    • Related Report
      2011 Final Research Report
  • [Journal Article] Development of cellprocessing systems for human stem cells(neural stem cells, mesenchymal stem cells, and iPS cells) for regenerative medicine2010

    • Author(s)
      Kanemura Y
    • Journal Title

      Keio J Med

      Volume: 59(2) Pages: 35-45

    • NAID

      10029469930

    • URL

      http://www.kjm.keio.ac.jp/past/59/2/index.html

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 細胞凝集塊形成プロセス制御による幹細胞の培養・分化制御2010

    • Author(s)
      金村米博,森英樹,八尋寛司,中澤浩二
    • Journal Title

      再生医療

      Volume: 9(3) Pages: 47-52

    • Related Report
      2011 Final Research Report
  • [Journal Article] Transplantation of Galectin-1-Expressing Human Neural Stem Cells Into the Injured Spinal Cord of Adult Common Marmosets.2010

    • Author(s)
      Yamane J, Nakamura M, Iwanami A, Sakaguchi M, Katoh H, Yamada M, Momoshima S, Miyao S, Ishii K, Tamaoki N, Nomura T, Okano HJ, Kanemura Y, Toyama Y, Okano H.
    • Journal Title

      J Neurosci Res

      Volume: 88 Pages: 1394-1405

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of cell-processing systems for human stem cells (neural stem cells, mesenchymal stem cells, and iPS cells) for regenerative medicine.2010

    • Author(s)
      Kanemura Y
    • Journal Title

      Keio J Med

      Volume: 59 Pages: 35-45

    • NAID

      10029469930

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞凝集塊形成プロセス制御による幹細胞の培養・分化制御2010

    • Author(s)
      金村米博、森英樹、八尋寛司、中澤浩二
    • Journal Title

      再生医療

      Volume: 9 Pages: 47-52

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 自己腫瘍ライセートをエレクトロポレーション法で取り込ませた樹状細胞はIFN-γ産生細胞の誘導を高める2010

    • Author(s)
      高原将司, 金村米博, 贄田美江, 前川隆司, 森内秀祐
    • Journal Title

      Biotherapy 24

      Pages: 21-26

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 神経幹細胞樹立に使用可能な細胞ソースと細胞品質管理2010

    • Author(s)
      金村米博
    • Journal Title

      実験医学増刊 28

      Pages: 75-82

    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒトiPS細胞由来神経幹細胞を応用した薬物安全性評価システムの開発(ヒトiPS細胞を用いた新規in vitro毒性評価系の構築)2012

    • Author(s)
      金村米博
    • Organizer
      スーパー特区フォーラムin大阪
    • Place of Presentation
      豊中市
    • Year and Date
      2012-01-19
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒトiPS細胞由来神経幹細胞を応用した薬物安全性評価システムの開発(ヒトipS細胞を用いた新規in vitro毒性評価系の構築)2012

    • Author(s)
      金村米博
    • Organizer
      スーパー特区フォーラムin大阪
    • Place of Presentation
      大阪府豊中市(招待講演)
    • Year and Date
      2012-01-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 悪性グリオーマの分子診断体制と細胞免疫治療法の開発2012

    • Author(s)
      金村米博
    • Organizer
      文部科学省橋渡し研究支援推進プログラム・橋渡し研究ネットワーク構築事業シンポジウム西日本のトランスレーショナルリサーチネットワーキングで活性化-" ACT West"基盤整備とプロジェクト展開
    • Place of Presentation
      吹田市
    • Year and Date
      2012-01-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 悪性グリオーマの分子診断体制と細胞免疫治療法の開発2012

    • Author(s)
      金村米博
    • Organizer
      文部科学省橋渡し研究支援推進プログラム・橋渡し研究ネットワーク構築事業シンポジウム西日本のトランスレーショナルリサーチネットワーキングで活性化-"ACT West"基盤整備とプロジェクト展開
    • Place of Presentation
      大阪府吹田市(招待講演)
    • Year and Date
      2012-01-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] iPS in neurooncolgy2011

    • Author(s)
      Kanemura Y
    • Organizer
      Taipei VGH and NYMU Brain Tumor Symposium 2011
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2011-11-30
    • Related Report
      2011 Final Research Report
  • [Presentation] iPS in neurooncolgy2011

    • Author(s)
      Kanemura Y
    • Organizer
      Taipei VGH and NYMU Brain Tumor Symposium 2011
    • Place of Presentation
      Taipei, Taiwan(招待講演)
    • Year and Date
      2011-11-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトiPS細胞を応用したヒトグリオーマ幹細胞の薬剤感受性評価2011

    • Author(s)
      金村米博,正札智子,兼松大介,松本有佳子,山本篤世,埜中正博,森内秀祐,中島伸,末水洋志,中村雅登,岡田洋平,岡野栄之,山崎麻美
    • Organizer
      第29回日本脳腫瘍学会学術集会
    • Place of Presentation
      岐阜県下呂市
    • Year and Date
      2011-11-27
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 膠芽腫におけるMGMT遺伝子promoter領域メチル化の定量的評価の試み2011

    • Author(s)
      森鑑二, 金村米博, 正札智子, 泉本修一, 埜中正博, 森内秀祐, 中島伸, 山崎麻美, 有田憲生
    • Organizer
      第29回日本脳腫瘍学会学術集会
    • Place of Presentation
      岐阜県下呂市
    • Year and Date
      2011-11-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高齢者悪性神経膠腫に対する遺伝子解析とテーラーメード治療への応用の検討2011

    • Author(s)
      角野喜則, 埜中正博, 金村米博, 正札智子, 馬場庸平, 永野大輔, 山崎麻美, 中島伸
    • Organizer
      社団法人日本脳神経外科学会第70回学術総会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2011-10-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトiPS細胞の実用化を支援する周辺技術開発2011

    • Author(s)
      金村米博
    • Organizer
      次世代医療システム産業化フォーラム
    • Place of Presentation
      大阪市
    • Year and Date
      2011-05-25
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒトiPS細胞の実用化を支援する周辺技術開発2011

    • Author(s)
      金村米博
    • Organizer
      次世代医療システム産業化フォーラム
    • Place of Presentation
      大阪市(招待講演)
    • Year and Date
      2011-05-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 樹状細胞を用いた悪性グリオーマに対する免疫療法-エレクトロポレーションを用いた腫瘍抗原導入法の可能性-2011

    • Author(s)
      金村米博
    • Organizer
      第10回日本再生医療学会総会ランチョンセミナー「細胞医療の新たなステージ~Cell Loading Systemの可能性~」
    • Place of Presentation
      東京
    • Year and Date
      2011-03-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] 神経幹細胞樹立に使用可能な細胞ソースと細胞品質管理および創薬研究への応用2011

    • Author(s)
      金村米博
    • Organizer
      第10回ヒューマンサイエンス研究資源バンクセミナー「培養細胞を利用した創薬研究-ここまで進んだバリデーション-」
    • Place of Presentation
      豊中市
    • Year and Date
      2011-01-28
    • Related Report
      2011 Final Research Report
  • [Presentation] 神経幹細胞樹立に使用可能な細胞ソースと細胞品質管理および創薬研究への応用2011

    • Author(s)
      金村米博
    • Organizer
      第10回ヒューマンサイエンス研究資源バンクセミナー「培養細胞を利用した創薬研究-ここまで進んだバリデーション-」
    • Place of Presentation
      大阪
    • Year and Date
      2011-01-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] In vitro chemosensitivity of human glioma stem cells compared with that of normal neural stem cells from human iPS cells2011

    • Author(s)
      Kanemura Y, Shofuda T, Kanematsu D, Matsumoto Y, Yamamoto A, Nonaka M, Moriuchi S, Nakajima S, Suemizu H, Nakamura M, Okada Y, Okano H, Yamasaki M
    • Organizer
      2011 SNO 16th Annual Scientific Meeting
    • Place of Presentation
      Orange County, California, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] In vitro chemosensitivity of human glioma stem cells compared with that of normal neural stem cells from human iPS cells2011

    • Author(s)
      Kanemura Y, Shofuda T, Kanematsu D, Matsumoto Y, Yamamoto A, Nonaka M, Moriuchi S, Nakajima S, Suemizu H, Nakamura M, Okada Y, Okano H, Yamasaki M
    • Organizer
      2011 SNO 16th Annual Scientific Meeting
    • Place of Presentation
      California, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトグリオーマ幹細胞の細胞特性解析と薬剤感受性評価2010

    • Author(s)
      金村米博
    • Organizer
      第28回日本脳腫瘍学会学術集会
    • Place of Presentation
      長野県北佐久郡軽井沢町
    • Year and Date
      2010-11-28
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒトグリオーマ幹細胞の細胞特性解析と薬剤感受性評価2010

    • Author(s)
      金村米博
    • Organizer
      第28回日本脳腫瘍学会学術集会
    • Place of Presentation
      軽井沢
    • Year and Date
      2010-11-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト神経幹細胞の細胞特性解析とその品質管理指標2010

    • Author(s)
      金村米博,正札智子,岡田洋平,岡野栄之,山崎麻美
    • Organizer
      社団法人日本脳神経外科学会第69回学術集会
    • Place of Presentation
      福岡市
    • Year and Date
      2010-10-28
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト神経幹細胞の細胞特性解析とその品質管理指標2010

    • Author(s)
      金村米博、正札智子、岡田洋平、岡野栄之、山崎麻美
    • Organizer
      社団法人日本脳神経外科学会第69回学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Stem cell research of congenital central nervous system malformations in pediatric neurosurgery2010

    • Author(s)
      Kanemura Y
    • Organizer
      International Symposium on Fetal Neurology
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2010-10-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Stem cell research of congenital central nervous system malformations in pediatric neurosurgery.2010

    • Author(s)
      Kanemura Y
    • Organizer
      International Symposium on Fetal Neurology
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2010-10-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 神経幹細胞とiPS細胞の基礎2010

    • Author(s)
      金村米博
    • Organizer
      International Symposium on Fetal Neurology
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2010-10-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dendritic cells loaded with autologous tumor lysate by electoporation effectively induce lysate specific IFN-γproducing T cells in glioblastoma patients2010

    • Author(s)
      Takahara M, Tomiyama M, Nieda M, Goto S, Peshwa MV, Kanemra Y, Maekawa R, Moriuchi S
    • Organizer
      AACR 101st Annual Meeting 2010
    • Place of Presentation
      Washington, DC, USA
    • Year and Date
      2010-04-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Dendritic cells loaded with autologous tumor lysate by electoporation effectively induce lysate specific IFN-γ producing T cells in glioblastoma patients.2010

    • Author(s)
      Takahara M, Tomiyama M, Nieda M, Goto S, Peshwa MV, Kanemra Y, Maekawa R, Moriuchi S.
    • Organizer
      AACR 101st Annual Meeting 2010
    • Place of Presentation
      Washington, DC, USA
    • Year and Date
      2010-04-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒトニューロスフェアの細胞特性とその品質管理指標2010

    • Author(s)
      金村米博
    • Organizer
      第9回日本再生医療学会総会
    • Place of Presentation
      広島市
    • Year and Date
      2010-03-18
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Stem cell research of congenital central nervous system abnormalities in pediatric neurosurgery2010

    • Author(s)
      Kanemura Y
    • Organizer
      Neurosurgery Update 2010 "Induced pluripotent stem cells"
    • Place of Presentation
      Seoul National University College of Medicine, Seoul, Korea
    • Year and Date
      2010-03-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Stem cell research of congenital central nervous system abnormalities in pediatric neurosurgery.2010

    • Author(s)
      金村米博
    • Organizer
      Neurosurgery Update 2010 "Induced pluripotent stem cells", Seoul National University College of Medicine
    • Place of Presentation
      ソウル(韓国)
    • Year and Date
      2010-03-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] 悪性グリオーマ由来腫瘍ライセートをエレクトロポレーション法で導入した樹状細胞は抗原特異的IFNγ産生細胞の誘導を高める2009

    • Author(s)
      Takahara M, Tomiyama M, Nieda M, Goto S, Kanemura Y, Maekawa R, Moriuch S
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪市
    • Year and Date
      2009-12-04
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] TMZ不応性再発悪性グリオーマに対する樹状細胞療法とLAK療法併用による免疫化学療法2009

    • Author(s)
      森内秀祐, 埜中正博, 金村米博, 明山耀久, 高垣匡寿, 山田公人, 出原誠, 伊藤守, 種子田護
    • Organizer
      第27回日本脳腫瘍学会
    • Place of Presentation
      泉佐野市
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Book] 悪性グリオーマに対する樹状細胞ワクチン療法の臨床研究について-免疫細胞治療II2011

    • Author(s)
      金村米博
    • Publisher
      幻冬舎メディアコンサルティング
    • Related Report
      2011 Final Research Report
  • [Book] 免疫細胞治療II(安元公正監修)(「悪性グリオーマに対する樹状細胞ワクチン療法の臨床研究について」)2011

    • Author(s)
      金村米博
    • Publisher
      幻冬舎メディアコンサルティング
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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