Development of new drugs for glioma stem cells in using human neural stem cells as in vitro model cells for drug screening and toxicity studies
| Project/Area Number |
21591888
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | National Hospital Organization Osaka National Hospital Institute for Clinical Reserch |
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Principal Investigator |
KANEMURA Yonehiro 独立行政法人国立病院機構大阪医療センター(臨床研究センター), 先進医療研究開発部再生医療研究室, 室長 (80344175)
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| Co-Investigator(Kenkyū-buntansha) |
SHOFUDA Tomoko 独立行政法人国立病院機構大阪医療センター(臨床研究センター), 先進医療研究開発部・幹細胞医療研究室, 室長 (40450895)
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| Co-Investigator(Renkei-kenkyūsha) |
MORIUCHI Shusuke りんくう総合医療センター, 脳神経外科, 部長 (90322180)
NAITO Takeaki 神戸薬科大学, 薬学部, 教授 (00068339)
MIYATA Okiko 神戸薬科大学, 薬学部, 教授 (90102110)
ARITA Norio 兵庫医科大学, 医学部, 教授 (80159508)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 脳腫瘍学 / グリオーマ幹細胞 / 脳神経疾患 / 薬剤反応性 / 癌 |
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| Research Abstract |
In this research, to develop new drugs for malignant gliomas(MGs), we examined the in vitro chemosensitivity of glioma stem cells(GSCs) compared with normal neural stem cells(NSCs), which we used in vitro model cells for drug screening and toxicity studies. We isolated three GSC lines isolated from surgically removed MG tissues and examined their detailed in vitro cellular properties. In vitro chemosensitivity to drugs for MGs(ACNU, TMZ, INF-β, VCR, PCZ) or other compounds against GSCs and NSCs were examined. We found that both GSCs and NSCs showed similar chemosensitivities and several compounds showed significant cytotoxic effect to GCSs and NSCs equally. These findings will increase our knowledge about the cellular properties of GSCs and provide useful information for the development of new drugs that target GSCs.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Methamphetamine induces endoplasmic reticulum stress related gene CHOP/Gadd153/ddit3 in dopaminergic cells2011
Author(s)
Irie, Yasuyuki; Saeki, Makio; Tanaka, Hidekazu; Kanemura, Yonehiro; Otake, Shinpei; Ozono, Yoshiyuki; Nagai, Toshisaburou; Kondo, Yukiko; Kudo, Kenzo; Kamisaki, Yoshinori; Miki, Naomasa; Taira, Eiichi
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Journal Title
Cell and Tissue Research
Volume: Vol.345:2
Issue: 2
Pages: 231-241
DOI
Related Report
Peer Reviewed
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[Journal Article] Transplantation of Galectin-1-Expressing Human Neural Stem Cells Into the Injured Spinal Cord of Adult Common Marmosets.2010
Author(s)
Yamane J, Nakamura M, Iwanami A, Sakaguchi M, Katoh H, Yamada M, Momoshima S, Miyao S, Ishii K, Tamaoki N, Nomura T, Okano HJ, Kanemura Y, Toyama Y, Okano H.
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Journal Title
J Neurosci Res
Volume: 88
Pages: 1394-1405
Related Report
Peer Reviewed
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[Presentation] ヒトiPS細胞を応用したヒトグリオーマ幹細胞の薬剤感受性評価2011
Author(s)
金村米博,正札智子,兼松大介,松本有佳子,山本篤世,埜中正博,森内秀祐,中島伸,末水洋志,中村雅登,岡田洋平,岡野栄之,山崎麻美
Organizer
第29回日本脳腫瘍学会学術集会
Place of Presentation
岐阜県下呂市
Year and Date
2011-11-27
Related Report
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[Presentation] In vitro chemosensitivity of human glioma stem cells compared with that of normal neural stem cells from human iPS cells2011
Author(s)
Kanemura Y, Shofuda T, Kanematsu D, Matsumoto Y, Yamamoto A, Nonaka M, Moriuchi S, Nakajima S, Suemizu H, Nakamura M, Okada Y, Okano H, Yamasaki M
Organizer
2011 SNO 16th Annual Scientific Meeting
Place of Presentation
Orange County, California, USA
Related Report
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[Presentation] In vitro chemosensitivity of human glioma stem cells compared with that of normal neural stem cells from human iPS cells2011
Author(s)
Kanemura Y, Shofuda T, Kanematsu D, Matsumoto Y, Yamamoto A, Nonaka M, Moriuchi S, Nakajima S, Suemizu H, Nakamura M, Okada Y, Okano H, Yamasaki M
Organizer
2011 SNO 16th Annual Scientific Meeting
Place of Presentation
California, USA
Related Report
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