Selective induction and transplantation of iPS cell-derived astrocytes into acute spinal cord injury

• Project/Area Number: 21591892
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Chiba University
• Principal Investigator: MURATA Atsushi 千葉大学, 医学部・附属病院, 准教授 (20344997)
• Co-Investigator(Kenkyū-buntansha): OKAWA Akihiko 千葉大学, 医学部附属病院, 講師 (30312945) ; YAMAZAKI Masashi 千葉大学, 大学院・医学研究院, 准教授 (50281712)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: iPS細胞 / 脊髄損傷 / Neural Stem Sphere法(NSS法) / アストロサイト / アロディニア / BBBスコア / NSS法 / rat / astrocyte / ACM / allodynia / BBB score

Research Abstract

We transplanted iPS cell-derived astrocytes into rat spinal cords three and seven days after spinal cord contusion injury. BBB locomotor recovery did not show statistically significant change in both transplantations. Although transplanted cells were survived eight weeks after transplantation, they did not show immunoreactivity for GFAP. There was no statistical significant histological finding. Although transplanted astrocytes has relation to induce allodynia, it depends on the timing of transplantation.

Research Products

• [Journal Article] Increase of sensitivity to mechanicalstimulus after transplantation of murineinduced pluripotent stem cell-derived astrocytes in a rat spinal cord injurymodel (2011)
  • Author(s): Hayashi K, Hashimoto M, Koda M, Naito AT, Murata A, Okawa A, Takahashi K, Yamazaki M
  • Journal Title: J Neurosurg Spine Volume: 15(6) Pages: 582-93
• [Journal Article] Increase of sensitivity to mechanical stimulus after transplantation of murine induced pluripotent stem cell-derived astrocytes in a rat spinalcord injury model (2011)
  • Author(s): Hayashi K, Hashimoto M, Koda M, Naito AT, Murata A, Okawa A, Takahashi K, Yamazaki M
  • Journal Title: J Neurosurg Spine Volume: 15 Pages: 582-593
  • Peer Reviewed
• [Journal Article] Neuroprotective effects of granulocyte colony-stimulating factor and relat ionship to promotion of angiogenesis after spinal cord injury in rats : laboratory investigation (2011)
  • Author(s): Kawabe J, Koda M, Hashimoto M, Fujiyoshi T, Furuya T, Endo T, Okawa A, Yamazaki M
  • Journal Title: J Neurosurg Spine Volume: 15 Pages: 414-421
  • Peer Reviewed
• [Journal Article] Transplantation of human bone marrow stromal cell-derived Schwann cells reduces cystic cavity and promotes functional recovery after contusion injury of adult rat spinal cord. (2011)
  • Author(s): Kamada T, Okawa A, YamazakiM.
  • Journal Title: Neuropathology Volume: 31 Pages: 48-58
  • Peer Reviewed
• [Journal Article] Treatment of rat spinal cord injury with a Rho-kinase inhibitor 3 and bone marrow stromal cell transplantation (2009)
  • Author(s): 大河昭彦、村田淳、山崎正志
  • Journal Title: Brain Research 1295 Pages: 192-202
  • Peer Reviewed
• [Presentation] ラット脊髄損傷に対するマウス人工多能性幹(iPS)細胞由来Astrocyte移植効果 (2010)
  • Author(s): 林浩一, 山崎正志, 大河昭彦, 村田淳
  • Organizer: 日本整形外科基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-15
• [Presentation] ラット脊髄損傷に対するマウス人工多能性幹(iPS)細胞由来Astrocyte移植効果 (2010)
  • Author(s): 林浩一, 山崎正志, 大河昭彦, 村田淳
  • Organizer: 日本再生医療学会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-19
• [Presentation] ラット脊髄損傷に対するマウス人工多能性幹(iPS)細胞由来Astrocyte移植効果 (2009)
  • Author(s): 林浩一, 山崎正志, 大河昭彦, 村田淳
  • Organizer: 日本整形外科基礎学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-11-05
• [Presentation] ラット脊髄損傷に対するマウス人工多能性幹(iPS)細胞由来Astrocyte移植効果 (2009)
  • Author(s): 大河昭彦、村田淳、山崎正志
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 横浜国際フォーラム(横浜)
  • Year and Date: 2009-11-05