• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Improvement of functional recovery and promotion of remyelination by cell transplantation and scaffold at the chronic phase after spinal cord injury

Research Project

Project/Area Number 21591894
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTokyo Medical and Dental University

Principal Investigator

ENOMOTO Mitsuhiro  東京医科歯科大学, 大学院・医歯学総合研究科, 寄附講座教員 (90451971)

Co-Investigator(Kenkyū-buntansha) WAKABAYASHI Yoshiaki  東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (00431916)
SOTOME Shinichi  東京医科歯科大学, 大学院・医歯学総合研究科, 寄付講座准教授 (20401391)
Co-Investigator(Renkei-kenkyūsha) SHINOMIYA Kenichi  東京医科歯科大学, 大学院・医歯学総合研究科, 名誉教授 (20111594)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords脊髄損傷 / 神経再生 / 神経栄養因子 / 神経幹細胞 / 移植 / 担体
Research Abstract

In this study, neural progenitor cells(NPCs) or bone marrow stromal cells(BMCs) combined with honeycomb collagen scaffold(HC) were transplanted into the injured spinal cord. HC with NPCs were implanted into contused spinal cord at six weeks after the initial thoracic injury. T
he functional recovery was little shown in the both implanted groups. On the other hand, rats implanted with HC containing BMCs displayed better hindlimb function and hypersensitivity than those implanted with HC without BMCs in the hemisected model. The implanted area exhibited more nerve fibers than the areas not containing BMCs. These effects result from the presence of BMCs that directly promote nerve regeneration. However, most of spinal cord injury patients have already paralyzed and maintained into chronic phase of recovery. Further treatments such as resection of scar tissue, developments of transplantation technique or rehabilitation would be needed to promote functional recovery at the chronic phase after spinal cord injury.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (38 results)

All 2012 2011 2010 2009 Other

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (12 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Subcutaneous injections ofplatelet-rich plasma into skin flapsmodulate proangiogenic gene expression and improve survival rates2012

    • Author(s)
      Li W, Enomoto M, Ukegawa M, Hirai T, Sotome S, Wakabayashi Y, Shinomiya K, Okawa A
    • Journal Title

      Plast Reconstr Surg

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Non-human primate model of amyotrophiclateral sclerosis with cytoplasmic mislocalization of TDP-432012

    • Author(s)
      Uchida A, Sasaguri H, Kimura N, TajiriM, Ohkubo T, Ono F, Sakaue F, Kanai K, Hirai T, Sano T, Shibuya K, Kobayashi M, Yamamoto M, Yokota S, Kubodera T, Tomori M, Sakaki K, Enomoto M, Hirai Y, Kumagai J, Yasutomi Y, Mochizuki H, Kuwabara S, Uchihara T, Mizusawa H, Yokota T
    • Journal Title

      Brain

      Volume: 135(Pt3) Pages: 833-46

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adhesive arachnoiditis with extensive syringomyelia and giant arachnoid cyst after spinal and epiduralanesthesia : a case report2012

    • Author(s)
      Hirai T, Kato T, Kawabata S, Enomoto M, Tomizawa S, Yoshii T, Sakaki K, ShinomiyaK, Okawa A
    • Journal Title

      Spine(Phila Pa1976)

      Volume: 37(3)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Five-year follow-upevaluation of surgical treatment forcervical myelopathy caused byossification of the posterior longitudinal ligament : a prospective comparative study of anterior decompression and fusion with floating method versus laminoplasty2012

    • Author(s)
      Sakai K, Okawa A, Takahashi M, Arai Y, Kawabata S, Enomoto M, Kato T, Hirai T, Shinomiya K
    • Journal Title

      Spine(Phila Pa1976)

      Volume: 37(5) Pages: 367-76

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Presence of anteriorcompression of the spinal cord after laminoplasty inhibits upper extremity motor recovery in patients with cervical spondylotic myelopathy2012

    • Author(s)
      Hirai T, Kawabata S, Enomoto M, Kato T, Tomizawa S, Sakai K, Yoshii T, Sakaki K, Shinomiya K, Okawa A
    • Journal Title

      Spine(Phila Pa1976)

      Volume: 37(5) Pages: 377-84

    • NAID

      10031169659

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subcutaneous injections of platelet-rich plasma into skin flaps modulate proangiogenic gene expression and improve survival rates2012

    • Author(s)
      Li W, Enomoto M, Sotome S, Wakabayashi Y, et al
    • Journal Title

      Plastic & Reconstructive Surgery

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-human primate model of amyotrophic lateral sclerosis with cytoplasmic mislocalization of TDP-432012

    • Author(s)
      Uchida A, Sasaguri H, Enomoto M, (18番目), et al
    • Journal Title

      Brain

      Volume: 135 Issue: 3 Pages: 833-46

    • DOI

      10.1093/brain/awr348

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adhesive arachnoiditis with extensive syringomyelia and giant arachnoid cyst after spinal and epidural anesthesia : a case report2012

    • Author(s)
      Hirai T, Kato T, Kawabata S, Enomoto M, et al
    • Journal Title

      SPINE

      Volume: 37 Issue: 3 Pages: 195-8

    • DOI

      10.1097/brs.0b013e31822ba817

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Five-year Follow-up Evaluation of Surgical Treatment for Cervical Myelopathy Caused by Ossification of the Posterior Longitudinal Ligament : A Prospective Comparative Study of Anterior Decompression and Fusion With Floating Method Versus Laminoplasty2012

    • Author(s)
      Sakai K, Okawa A, Takahashi M, Arai Y, Kawabata S, Enomoto M, et al
    • Journal Title

      SPINE

      Volume: 37 Issue: 5 Pages: 367-76

    • DOI

      10.1097/brs.0b013e31821f4a51

    • NAID

      10031169658

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Presence of anterior compression of the spinal cord after laminoplasty inhibits upper extremity motor recovery in patients with cervical spondylotic myelopathy2012

    • Author(s)
      Hirai T, Kawabata S, Enomoto M, et al
    • Journal Title

      SPINE

      Volume: 37 Issue: 5 Pages: 377-84

    • DOI

      10.1097/brs.0b013e31821fd396

    • NAID

      10031169659

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Middle-term results of a prospective comparative study of anteriorde compression with fusion and posterior decompression with laminoplasty for thetreatment of cervical spondylotic myelopathy2011

    • Author(s)
      Hirai T, Okawa A, Arai Y, Takahashi M, Kawabata S, Kato T, Enomoto M, Tomizawa S, Sakai K, Torigoe I, Shinomiya K
    • Journal Title

      Spine(Phila Pa1976)

      Volume: 36(23) Pages: 1940-7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of sciatic nerve regeneration by adenovirus-mediated expression ofdominant negative RhoA and Rac12011

    • Author(s)
      Kusano K, Enomoto M, Hirai T, Wakabayashi Y, Itoh S, Ichinose S, OkabeS, Shinomiya K, Okawa A
    • Journal Title

      Neurosci Lett

      Volume: 492(1) Pages: 64-9

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Risk factors forearly reconstruction failure of multilevel cervical corpectomy with dynamic plate fixation2011

    • Author(s)
      Okawa A, Sakai K, Hirai T, Kato T, Tomizawa S, Enomoto M, Kawabata S, Takahashi M, Shinomiya K
    • Journal Title

      Spine(Phila Pa1976)

      Volume: 36(9)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of sciatic nerve regeneration by adenovirus-mediated expression of dominant negative RhoA and Racl2011

    • Author(s)
      K.Kusano, M.Enomoto, T.Hirai, Y.Wakabayashi, S.Itoh, S.Ichinose, S.Okabe, K.Shinomiya, A.Okawa
    • Journal Title

      Neuroscience Letters

      Volume: 492 Pages: 64-64

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Middle-Term Results of a Prospective Comparative Study of Anterior Decompression with Fusion and Posterior Decompression with Laminoplasty for the Treatment of Cervical Spondylotic Myelopathy2011

    • Author(s)
      T.Hirai, A.Okawa, Y.Arai, M.Takahashi, S.Kawabata, T.Kato, M.Enomoto, S.Tomizawa, K.Sakai, I.Torigoe, K.Shinomiya
    • Journal Title

      Spine

    • NAID

      10030901171

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diagnosis of incomplete conduction block of spinal cord from skinsurface using spinal cord evoked magnetic fields2010

    • Author(s)
      Tomori M, Kawabata S, Tomizawa S, Ishii S, Enomoto M, Adachi Y, Sato T, Shinomiya K, Okawa A
    • Journal Title

      J Orthop Sci

      Volume: 15(3) Pages: 371-80

    • NAID

      10027404129

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transplanted neural progenitor cells expressing mutant NT3 promote myelination and partial hindlimb recovery in thechronic phase after spinal cord injury2010

    • Author(s)
      Sotome S, Shinomiya K, Okawa A
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 393(4) Pages: 812-7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diagnosis of incomplete conduction block of spinal cord from skin surface using spinal cord evoked magnetic fields2010

    • Author(s)
      M.Tomori, S.Kawabata, S.Tomizawa, S.Ishii, M.Enomoto, Y.Adachi, T.Sato, K.Shinomiya, A.Okawa
    • Journal Title

      Journal of Orthopaedic Science

      Volume: 15 Pages: 3719-3719

    • NAID

      10027404129

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transplanted neural progenitor cells expressing mutant NT3 promote myelination and partial hindlimb recovery in the chronic phase after spinal cord injury2010

    • Author(s)
      Kusano K, Enomoto M, Hirai T, Tsoulfas P, Sotome S, Shinomiya K, Okawa A
    • Journal Title

      Biochemical and Biophysical Research Communications 393

      Pages: 812-817

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Critical involvementof Rho GTPase activity in the efficienttransplantation of neural stem cells intothe injured spinal cord2009

    • Author(s)
      Numano F, Inoue A, Enomoto M, Shinomiya K, Okawa A, Okabe S
    • Journal Title

      Mol Brain

      Volume: 2 Pages: 37-37

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Critical involvement of Rho GTPase activity in the efficient transplantation of neural stem cells into the injured spinal cord.2009

    • Author(s)
      Numano F, Inoue A, Enomoto M, Shinomiya K, Okawa A, Okabe S
    • Journal Title

      Molecular brain 2:37

      Pages: 1-16

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Axonal regeneration into a honey combcollagen sponge combined with bone marrow stromal cells in the injured spinal cord2011

    • Author(s)
      M. Enomoto, M. Ukegawa, K. Bhatt, T. Hirai, S. Sotome, K. Shinomiya, A. Okawa
    • Organizer
      The 41th annual meeting of the Society for Neuroscience
    • Place of Presentation
      ワシントンDC
    • Year and Date
      2011-11-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Axonal regeneration into a honeycomb collagen sponge combined with bone marrow stromal cells in the injured spinal cord2011

    • Author(s)
      M.Enomoto, et al
    • Organizer
      The 41 th annual meeting of the Society for Neuroscience
    • Place of Presentation
      米国ワシントンDC
    • Year and Date
      2011-11-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] ハニカムコラーゲンスポンジと骨髄間葉系細胞移植を併用した損傷脊髄の再建2011

    • Author(s)
      榎本光裕、早乙女進一, ら
    • Organizer
      第26回日本整形外科学会基礎学術集会
    • Place of Presentation
      前橋(招待講演)
    • Year and Date
      2011-10-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] ハニカムコラーゲンスポンジと骨髄間葉系細胞移植を併用した損傷脊髄の再建2011

    • Author(s)
      榎本光裕,請川円,平井高志,早乙女進一,四宮謙一,大川淳
    • Organizer
      第26回日本整形外科学会基礎学術集会
    • Place of Presentation
      前橋
    • Related Report
      2011 Final Research Report
  • [Presentation] Enhancement of sciatic nerve regeneration by adenovirus-mediated expression of dominant negative RhoA and Rac12010

    • Author(s)
      M. Enomoto, K. Kusano, T. Hirai, Y. Wakabayashi, S. Itoh, A. Okawa, K. Shinomiya
    • Organizer
      The 40th annual meeting of the Society for Neuroscience
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2010-11-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Enhancement of sciatic nerve regeneration by adenovirus-mediated expression of dominant negative RhoA and Racl.2010

    • Author(s)
      M.Enomoto, K.Kusano, T.Hirai, Y.Wakabayashi, S.Itoh, A.Okawa, K.Shinomiya
    • Organizer
      The 40th annual meeting of the Society for Neuroscience
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2010-11-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 神経因性疼痛モデルマウスの腰部後根神経節でのTRP familyの経時的発現パターン2010

    • Author(s)
      平井高志,榎本光裕,早乙女進一,四宮謙一,大川淳
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      京都
    • Year and Date
      2010-10-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Mutant NT3を導入した神経前駆細胞移植による慢性期脊髄損傷治療の試み2009

    • Author(s)
      草野和生, 榎本光裕, 早乙女進一, 大川淳, 四宮謙一
    • Organizer
      第24回日本整形外科学会基礎学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Rho family Gprotein制御によるラット坐骨神経再生の促進2009

    • Author(s)
      草野和生,榎本光裕,若林良明,伊藤聰一郎,岡部繁男,四宮謙一
    • Organizer
      第24回日本整形外科学会基礎学術集会
    • Year and Date
      2009-11-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Mutant NT3を導入した神経前駆細胞移植による慢性期脊髄損傷治療の試み2009

    • Author(s)
      草野和生,榎本光裕,早乙女進一,大川淳,四宮謙一
    • Organizer
      第24回日本整形外科学会基礎学術集会
    • Year and Date
      2009-11-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Neurotrophic activities of transplanted neural progenitor cells expressing mutant NT3 ininjured spinal cord2009

    • Author(s)
      K. Kusano, M. Enomoto, P. Tsoulfas, K. Shinomiya, A. Okawa
    • Organizer
      The 39th annual meeting of the Society for Neuroscience
    • Place of Presentation
      シカゴ
    • Year and Date
      2009-10-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Neurotrophic activities of transplanted neural progenitor cells expressing mutant NT3 in injured spinal cord2009

    • Author(s)
      草野和生, 榎本光裕, 早乙女進一, 四宮謙一, 大川淳
    • Organizer
      The 39th annual meeting of the Society for Neuroscience
    • Place of Presentation
      Chicago
    • Year and Date
      2009-10-20
    • Related Report
      2009 Annual Research Report
  • [Book] CLINICAL AND BASIC SCIENCEPERSPECTIVES Nova Science Publishers2012

    • Author(s)
      Mitsuhiro Enomoto and Aldrich Hama
    • Publisher
      PAIN MANAGEMENT FOLLOWING SPINAL CORDINJURY
    • Related Report
      2011 Final Research Report
  • [Book] PAIN MANAGEMENT FOLLOWING SPINAL CORD INJURY : CLINICAL AND BASIC SCIENCE PERSPECTIVES2012

    • Author(s)
      Enomoto M, Hama AT
    • Total Pages
      40
    • Publisher
      Nova Science Publishers
    • Related Report
      2011 Annual Research Report
  • [Remarks] 東京医科歯科大学整形外科HP

    • URL

      http://www.tmd.ac.jp/med/orth/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks] 東京医科歯科大学整形外科HP

    • URL

      http://www.tmd.ac.jp/med/orth/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks] 東京医科歯科大学整形外科HP

    • URL

      http://www.tmd.ac.jp/med/orth/orth-J.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi