Basic science technology study of new revision total hip arthroplasty using a fusion protein and the nanowire processing

• Project/Area Number: 21591923
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kitasato University
• Principal Investigator: NARUSE Kouji 北里大学, 医学部, 講師 (60276087)
• Co-Investigator(Kenkyū-buntansha): ITOMAN Moritoshi 北里大学, 名誉教授 (70104528) ; URABE Ken 北里大学, 医学部, 准教授 (90284489) ; MATSUSHITA Osamu 北里大学, 医学部, 教授 (00209537)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 材料工学・処理 / 組織移植 / 融合タンパク / 関節機能再建術 / 金属表面加工 / 副甲状腺ホルモン / 繊維芽細胞成長因子

Research Abstract

In this study we aimed to develop a new reconstruction technique for widespread bone defects that occur during revision and second revision total hip arthroplasty. We strived to significantly improve joint function restoration techniques that are currently based on existing bone allograft. prosthesis composite materials by using hormone-collagen binding domain and growth factor. collagen binding domain as molecular biological tools, as well as metal. plated nanowire processing technology as a medical engineering tool. Our study revealed that basic fibroblast growth factors. collagen binding domain were useful in the development of the new reconstruction technique.

Research Products

• [Journal Article] Differential age-related bone architecture changes between female and male STR/Ort mice. (2011)
  • Author(s): Kentaroo Uchida
  • Journal Title: Exp Anim. Volume: 61 Pages: 59-66
  • Peer Reviewed
• [Journal Article] Low intensity pulsed ultrasound accelerates delayed healing process by reducing the time required for the completion of endochondral ossification in the aged mouse femur fracture model. (2011)
  • Author(s): Motoaki Katano
  • Journal Title: Exp Anim. Volume: 60 Pages: 385-395
  • Peer Reviewed
• [Journal Article] A novel underuse model shows that inactivity but not ovariectomy determines the deteriorated material properties and geometry of cortical bone in the tibia of adult rats. (2011)
  • Author(s): Miyagawa K
  • Journal Title: J Bone Miner Metab. Volume: 29 Pages: 422-436
  • Peer Reviewed
• [Journal Article] Low-intensity pulsed ultrasound promotes the osteogenic effect induced by bone allograft (2011)
  • Author(s): Jun Aikawa
  • Journal Title: Kitasato Medical journal Volume: 41 Pages: 184-192
  • Peer Reviewed
• [Journal Article] Inactivity but Not ovariectomy Determines the mechanical property and Quality of Bone in the Hind Limbs of Aged Female Rats (2011)
  • Author(s): Suto Mitsutoshi, Kouji Naruse
  • Journal Title: Kitasto Medical Journal Volume: 41 Pages: 76-83
  • Peer Reviewed
• [Journal Article] Influence of internal fixator stiffness on murine fracture healing : two types of fracture healing lead to two distinct cellular events and fgf-2 expressions. (2010)
  • Author(s): Masaki Ueno, Kouji Naruse
  • Journal Title: Exp Anim. Volume: 60 Pages: 79-87
  • NAID: 10029550016
  • Peer Reviewed
• [Journal Article] Repeated freeze-thaw cycles reduce the survival rate of osteocytes in bone-tendon constructs without affecting the mechanical properties of tendons. (2010)
  • Author(s): Kaori Suto, Kouji Naruse
  • Journal Title: Cell Tissue Bank. Volume: 30
  • Peer Reviewed
• [Journal Article] Umbilical cord blood-derived mesenchymal cell fate after mouse umbilical cord blood transplantation. (2010)
  • Author(s): Ken Uchida, Kouji Naruse
  • Journal Title: Transplantation. Volume: 90 Pages: 1037-1039
  • Peer Reviewed
• [Journal Article] Prolonged endochondral bone healing in senescence is shortened by low-intensity pulsed ultrasound in a manner dependent on COX-2. (2010)
  • Author(s): Kouji Naruse
  • Journal Title: Ultrasound Med Biol. Volume: 36 Pages: 1098-1108
  • Peer Reviewed
• [Journal Article] A new technique for seeding chondrocytes onto solvent-preserved human meniscus using the chemokinetic effect of recombinant human BMP-2. (2010)
  • Author(s): Hiroaki Minehara, Kouji Naruse
  • Journal Title: Cell Tissue Bank Volume: 17
  • Peer Reviewed
• [Journal Article] 短時間で効率的な骨髄成分除去方法の検討 (2010)
  • Author(s): 松尾昌、成瀬康治
  • Journal Title: 移植 Volume: 14 Pages: 303-304
  • Peer Reviewed
• [Journal Article] リン酸カルシウム骨セメントを用いた抗菌薬のdrug delivery system とコンポーネントを温存した人工膝関節全置換術後感染の治療 (2010)
  • Author(s): 占部憲、成瀬康治
  • Journal Title: 整形・災害外科 Volume: 53 Pages: 603-610
• [Journal Article] マウス臍帯血移植モデル (2010)
  • Author(s): 内田健太郎、成瀬康治
  • Journal Title: CRJ LETTEES Volume: 19 Pages: 11-15
• [Journal Article] STR/ortCrljマウスの病態とその評価 (2010)
  • Author(s): 内田健太郎、成瀬康治
  • Journal Title: CRJ LETTEES Volume: 19 Pages: 1-10
• [Journal Article] In vitro comparison of elution characteristics of vancomycin from calcium phosphate cement and polymethylmethacrylate. (2009)
  • Author(s): Urabe K, Naruse K
  • Journal Title: J Orthop Sci. 14(6) Pages: 784-793
  • NAID: 10027402486
  • Peer Reviewed
• [Journal Article] Prior treatment with vitamin K(2) significantly improves the efficacy of risedronate. (2009)
  • Author(s): Matsumoto Y, Naruse K
  • Journal Title: Osteoporos Int. 20(11) Pages: 1863-1872
  • Peer Reviewed
• [Journal Article] Osteoarthritic changes of the patellofemoral joint in STR/OrtCrlj mice are the earliest detectable changes and may be caused by caused by internal tibial torsion. (2009)
  • Author(s): Naruse K, Urabe K
  • Journal Title: Connect Tissue Res. 50(4) Pages: 243-255
  • Peer Reviewed
• [Presentation] 下肢の新鮮骨折例と難治性骨折例に対する低出力超音波パルス治療効果の比較 (2012)
  • Author(s): 内野正隆
  • Organizer: 超音波骨折治療研究会
  • Place of Presentation: 東京
  • Year and Date: 2012-01-21
• [Presentation] 各種創傷被覆剤における低出力超音波パルスの超音波透過への影響 (2011)
  • Author(s): 成瀬康治
  • Organizer: 西日本整形災害外科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2011-07-02
• [Presentation] Influence an ultrasound transmission of low intensity pulsed ultrasound via various wound dressing products (2011)
  • Author(s): 成瀬康治
  • Organizer: 日本骨折治療学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-07-02
• [Presentation] 人工膝関節全置換術(TKA)後の大腿骨顆上骨折の観血的治療法の選択と術後治療成績について (2011)
  • Author(s): 平川紀子
  • Organizer: 神奈川骨折治療研究会
  • Place of Presentation: 横浜
  • Year and Date: 2011-06-04
• [Presentation] 変形性膝関節症マウスSTR/Ortにおける脂質代謝異常 (2011)
  • Author(s): 内田健太郎、成瀬康治
  • Organizer: 第24回日本軟骨代謝学会
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2011-03-05
• [Presentation] マウス臍帯血移植後に骨髄に生着した細胞は骨折治癒に関与する (2011)
  • Author(s): 内田健太郎、成瀬康治
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2011-03-01
• [Presentation] 骨折部不安定性遷延癒合モデルに対して低出力超音波パルスが無効である機序の検討 (2011)
  • Author(s): 成瀬康治
  • Organizer: 第14回超音波骨折治療研究会
  • Place of Presentation: 東京ステーションコンファレンス(東京都)
  • Year and Date: 2011-01-22
• [Presentation] インプラント界面に残留する定常荷重の骨組織への影響 (2010)
  • Author(s): 馬渕清資、成瀬康治
  • Organizer: 第37回日本臨床バイオメカニクス学会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2010-11-01
• [Presentation] LOW INTENSITY PULSED ULTRASOUND ACCELERATED ENDOCHONDRALV OSSIFICATIONIN AGED MOUSE FEMUR FRACTURE MODEL (2010)
  • Author(s): Kouji Naruse
  • Organizer: 13th International Conference of Asia Pacific Association of Surgical Tissue Banks (APASTB)
  • Place of Presentation: パタヤ(インドネシア)
  • Year and Date: 2010-10-29
• [Presentation] THE CURRENT STATUS AND ISSUE OF ADVANCED MEDICAL TREATMENT FOR ALLOGENIC BONE AND LIGAMENTOUS TISSUE IN JAPAN (2010)
  • Author(s): Ken Urabe, Kouji Naruse
  • Organizer: 13th International Conference of Asia Pacific Association of Surgical Tissue Banks (APASTB)
  • Place of Presentation: パタヤ(インドネシア)
  • Year and Date: 2010-10-29
• [Presentation] 北里大学における整形外科医育成のための試みと今後の課題 (2010)
  • Author(s): 内山勝文、成瀬康治
  • Organizer: 第140回神奈川整形災害外科研究会
  • Place of Presentation: 横浜ロイヤルパークホテル(神奈川県)
  • Year and Date: 2010-10-23
• [Presentation] Mesenchymal cell fate after mice cord blood transplantation (2010)
  • Author(s): Kentaroo Uchida, Kouji Naruse
  • Organizer: 7th Combined Meeting of the Orthopaedic Research Societies
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2010-10-16
• [Presentation] 運動器細胞代謝におけるメカニカルストレスの利点と欠点低出力超音波パルス(LIPUS)による骨折治癒促進効果の機序 (2010)
  • Author(s): 成瀬康治
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2010-10-14
• [Presentation] Abnormal tibial torsion may cause an earliest detectable deformity in STR/OrtCrlj osteoarthritis mouse model (2010)
  • Author(s): Kouji Naruse
  • Organizer: 9th World Congress of the International Cartilage Repair Society
  • Place of Presentation: バルセロナ(スペイン)
  • Year and Date: 2010-09-29
• [Presentation] Investigation of hyperlipidemic property in STR/Ort mice (2010)
  • Author(s): Kentaroo Uchida, Kouji Naruse
  • Organizer: 9th World Congress of the International Cartilage Repair Society
  • Place of Presentation: バルセロナ(スペイン)
  • Year and Date: 2010-09-29
• [Presentation] 冷凍・解凍の繰り返しによる骨・靭帯組織中の生存細胞除去 (2010)
  • Author(s): 須藤香織、成瀬康治
  • Organizer: 第29回 日本運動器移植・再生医学研究会
  • Place of Presentation: 京都テルサ(京都府)
  • Year and Date: 2010-09-25
• [Presentation] マウス臍帯血移植後の間葉系細胞の動態 (2010)
  • Author(s): 内田健太郎、成瀬康治
  • Organizer: 第29回 日本運動器移植・再生医学研究会
  • Place of Presentation: 京都テルサ(京都府)
  • Year and Date: 2010-09-25
• [Presentation] 同種軟骨組織のより適切な低温保存のための組織保存液の開発 (2010)
  • Author(s): 小沼賢治、成瀬康治
  • Organizer: 第9回 日本組織移植学会総会・学術集会
  • Place of Presentation: コラッセふくしま(福島県)
  • Year and Date: 2010-08-28
• [Presentation] 先進医療「非生体ドナーによる凍結保存同種骨・靭帯組織」の現状と問題点 (2010)
  • Author(s): 占部憲、成瀬康治
  • Organizer: 第9回 日本組織移植学会総会・学術集会
  • Place of Presentation: コラッセふくしま(福島県)
  • Year and Date: 2010-08-28
• [Presentation] 同種骨軟骨組織のより適切な低温保存のための組織保存液の開発 (2010)
  • Author(s): 小沼賢治、成瀬康治
  • Organizer: 第23回日本軟骨代謝学会
  • Place of Presentation: 鹿児島県医師会館(鹿児島県)
  • Year and Date: 2010-04-02
• [Book] 移植のための臓器摘出と保存 (2011)
  • Author(s): 浅野武秀
  • Total Pages: 304
  • Publisher: 丸善
• [Book] 移植のための臓器摘出と保存 (2011)
  • Author(s): 浅野武秀
  • Total Pages: 304
  • Publisher: 丸善出版
• [Patent(Industrial Property Rights)] 成長因子アンカーリング型骨移植材料、成長因子アンカーリング型骨移植材料の製造方法、成長因子アンカーリング型骨移植材料製造用キット、および骨形成方法 (2012)
  • Inventor(s): 内田健太郎、成瀬康治、高相晶士、美間健彦、松下治、原口高志、西望
  • Industrial Property Rights Holder: 北里大学、香川大学
  • Industrial Property Number: 2011-108650
  • Filing Date: 2012-03-26
• [Patent(Industrial Property Rights)] 成長因子アンカーリング型骨移植材料、成長因子アンカーリング型膏移植材料の製造方法、成長因子アンカーリング型骨移植材料製造用キット、および骨形成方法 (2012)
  • Inventor(s): 内田健太郎、成瀬康治、高相晶士、美間健彦、松下治、原口高志、西望
  • Industrial Property Rights Holder: 北里大学、香川大学
  • Filing Date: 2012-03-26
  • Overseas
• [Patent(Industrial Property Rights)] 成長因子アンカーリング型骨移植材料、成長因子アンカーリング型骨移植材料の製造方法、成長因子アンカーリング型骨移植材料製造用キット、および骨形成方法 (2011)
  • Inventor(s): 内田健太郎、成瀬康治、高相晶士、美間健彦、松下治、原口高志、西望
  • Industrial Property Rights Holder: 北里大学、香川大学
  • Filing Date: 2011-05-12
  • Overseas
• [Patent(Industrial Property Rights)] 成長因子アンカーリング型骨移植材料、成長因子アンカーリング型骨移植材料の製造方法、成長因子アンカーリング型骨移植材料製造用キット.および骨形成方法 (2011)
  • Inventor(s): 内田健太郎、成瀬康治、高相晶士、美間健彦、松下治、原口高志、西望
  • Industrial Property Rights Holder: 北里大学、香川大学
  • Industrial Property Number: 2011-108650
  • Filing Date: 2011-05-12