| Project/Area Number |
21591927
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
KITAMURA Nobuto 北海道大学, 大学院・医学研究科, 講師 (80447044)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Eiji 北海道大学, 大学院・医学研究科, 講師 (60374724)
YASUDA Kazunori 北海道大学, 大学院・医学研究科, 教授 (20166507)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 腱・靭帯組織 / 除負荷 / アポト-シス / 健・靭帯組織 / アポトーシス |
|---|
| Research Abstract |
The effect of stress deprivation on the tendon tissue has been an important focus in the field of biomechanics. Rabbits were divided into stress-shielded, sham-operated, and control groups. To completely shield the patellar tendon from stress, we used an established surgical method. Both the number and the ratio of TUNEL-, caspase-3-, JNK-, and p38-positive cells were significantly greater (p<0.0001) in the stress-shielded group than in the sham group at 24h, 2, and 4 days. This study demonstrated that complete stress deprivation induces fibroblast apoptosis in vivo with activation of JNK and p38 within 24h. This fact suggested that the fibroblast apoptosis caused by stress deprivation is induced via the mitogen-activated protein kinase signaling pathway.
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