Preclinical trial of less invasive synovial stem cell therapy using a pig knee articular cartilage defect model

• Project/Area Number: 21591937
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: MUNETA Takeshi 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (50190864)
• Co-Investigator(Kenkyū-buntansha): SEKIYA Ichiro 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (10345291) ; KOBAYASHI Eiji 自治医科大学, 医学部, 教授 (00245044)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: H :運動器外傷学 / 幹細胞治療 / 滑膜幹細胞 / 軟骨欠損 / 大動物研究 / 低侵襲手術 / 大型動物実験 / ミニブタ / 関節軟骨損傷 / 滑膜由来幹細胞 / 細胞治療

Research Abstract

Less invasive synovial stem cell transplantation promoted healing of a full-thickness articular cartilage defect at weight-bearing area in a mini pig model. The results facilitate to perform clinical trial of the transplantation on human patients with articular cartilage injury.

Research Products

• [Journal Article] Arthroscopic, histological and MRIanalyses of cartilage repair after aminimally invasive method oftransplantation of allogeneic synovialmesenchymal stromal cells into cartilagedefects in pigs (2012)
  • Author(s): Nakamura T, Sekiya I, Muneta T, Hatsushika D, Horie M, Tsuji K, Kawarasaki T, Watanabe A, Hishikawa S, Fujimoto Y, Tanaka H, Kobayashi E
  • Journal Title: Cytotherapy Volume: 14 Pages: 327-338
• [Journal Article] Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs (2012)
  • Author(s): Tomomasa Nakamura, Ichiro Sekiya, Takeshi Muneta, et al
  • Journal Title: Cytotherapy Volume: 14 Issue: 3 Pages: 327-338
  • DOI: 10.3109/14653249.2011.638912
  • Peer Reviewed
• [Journal Article] Differences in kinematics of single leg squatting between anterior cruciate ligament-injured patients and healthy controls. (2010)
  • Author(s): Yamazaki J, Muneta T, Ju YJ, Sekiya I.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc. 18(1) Pages: 56-63
  • Peer Reviewed
• [Journal Article] Morphological differences during in vitro chondrogenesis of bone marrow-, svnovium-MSCs, and chondrocvte. (2009)
  • Author(s): Ichinose S, Muneta, et al(2/6)
  • Journal Title: Lab Invest. 90(2) Pages: 210-221
  • Peer Reviewed
• [Journal Article] Methylation status of CpG islands in the promoter regions of signature genes during chondrogenesisof human synovium-derived mesenchymal stem cells. (2009)
  • Author(s): Ezura Y, Muneta T, et al (4/5)
  • Journal Title: Arthritis Rheum 60(5) Pages: 1416-1426
  • Peer Reviewed
• [Journal Article] Anatomy of normal human anterior cruciate ligament attachments evaluated by divided small bundles. (2009)
  • Author(s): Hara K, Mochizuki T, Sekiya I, Yamaguchi K, Akita K, Muneta T.
  • Journal Title: Am J Sports Med 37(12) Pages: 2386-2391
  • Peer Reviewed
• [Journal Article] Is a minimally invasive approach superior to a conventional approach for total knee arthroplasty? Early outcome and 2- to 4-year follow-up. (2009)
  • Author(s): Watanabe T, Muneta T, Ishizuki M.
  • Journal Title: J Orthop Sci. 14(5) Pages: 589-595
  • NAID: 10025588321
  • Peer Reviewed
• [Journal Article] Atelocollagen-associated autologous chondrocyte implantation for the repair of chondral defects of the knee : a prospective multicenter clinical trial in Japan. (2009)
  • Author(s): Tohyama H, Yasuda K, Minami A, Majima T, Iwasaki N, Muneta T, Sekiya I, Yagishita K, Takahashi S, Kurokouchi K, Uchio Y, Iwasa J, Deie M, Adachi N, Sugawara K, Ochi M.
  • Journal Title: J Orthop Sci. 14(5) Pages: 579-588
  • Peer Reviewed
• [Journal Article] In vivo pharmacokinetics of ketoprofen after patch application in the Mexican hairless pig. (2009)
  • Author(s): Horie M, Sekiya I, Nakamura T, Tanaka H, Maekawa K, Nakanishi M, Muneta T, Kobayashi E.
  • Journal Title: Biopharm Drug Dispos 30(4) Pages: 204-208
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cell-based therapy for cartilage repair : a review. (2009)
  • Author(s): Koga H, Engebretsen L, Brinchmann JE, Muneta T, Sekiya I.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc 17(11) Pages: 1289-1297
  • Peer Reviewed
• [Journal Article] Humeral insertion of the supraspinatus and infraspinatus. New anatomical findings regarding the footprint of the rotator cuff. Surgical technique. (2009)
  • Author(s): Mochizuki T, Sugaya H, Uomizu M, Maeda K, Matsuki K, Sekiya I, Muneta T, Akita K.
  • Journal Title: J Bone Joint Surg Aml ; 91 91
  • Peer Reviewed
• [Journal Article] Periodic knee injections of BMP-7 delay cartilage degeneration induced by excessive running in rats. (2009)
  • Author(s): Sekiya I, Tang T, Hayashi M, Morito T, Ju YJ, Mochizuki T, Muneta T.
  • Journal Title: J Orthop Res. 27(8) Pages: 1088-1092
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells derived from synovium, meniscus, anterior cruciate ligament, and articular chondrocytes share similar gene expression profiles. (2009)
  • Author(s): Segawa Y, Muneta T, Makino H, Nimura A, Mochizuki T, Ju YJ, Ezura Y, Umezawa A, Sekiya I.
  • Journal Title: J Orthop Res. 27(4) Pages: 435-441
  • Peer Reviewed
• [Journal Article] Biological fixation of fibrous materials to bone using chitin/chitosan as a bone formation accelerator. (2009)
  • Author(s): Kawai T, Yamada T, Yasukawa A, Koyama Y, Muneta T, Takakuda K.
  • Journal Title: J Biomed Mater Res B Appl Biomater. 88(1) Pages: 264-270
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 宗田大, 秋田恵一
  • Journal Title: 「手術進入法-下肢」最新整形外科学大系8 中山書店(第5章膝関節への進入法内側側方進入法) Pages: 212-217
• [Journal Article] (2009)
  • Author(s): 宗田大
  • Journal Title: Monthly Book Orthopaedics(前十字靭帯損傷の診断と治療。すぐに役立つ日常整形外科診療に対する私の工夫) Pages: 231-242
• [Presentation] ブタ軟骨欠損モデルにおける同種滑膜幹細胞移植 (2011)
  • Author(s): 中村智祐
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 群馬
  • Year and Date: 2011-10-21
• [Presentation] ブタ軟骨欠損モデルにおける同種滑膜幹細胞移植 (2011)
  • Author(s): 中村智祐、関矢一郎、宗田大, ほか
  • Organizer: 第26回日本整形外科学会基礎学術集会
• [Presentation] Synovial Mesenchymal Stem Cells Transplantation Contributes to Cartilage Regeneration in Porcine Model (2011)
  • Author(s): Nakamura T, Sekiya I, Hatsushika D, Horie M, Tsuji K, Muneta T, Kawarasaki T, Watanabe A, Hishikawa S, Fujimoto Y, Tanaka H, Kobayashi E.
  • Organizer: Orthopaedic ResearchSociety 2011
  • Place of Presentation: Long Beach
• [Presentation] Synovial Mesenchymal Stem Cells Transplantation Contributesto Cartilage Regeneration in Porcine Model (2011)
  • Author(s): Tomomasa Nakamura
  • Organizer: Orthopaedic Research Society 2011
  • Place of Presentation: Long Beach, CA
• [Presentation] ブタを用いた滑膜幹細胞による低侵襲軟骨再生治療:(GFP)関節鏡, 組織MRIによる評価 (2010)
  • Author(s): 中村智祐
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-15
• [Presentation] ブタ滑膜間葉幹細胞を用いた低侵襲軟骨再生治療の検討 (2010)
  • Author(s): 中村智祐
  • Organizer: 第29回日本運動器移植・再生医学研究会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-25
• [Presentation] 滑膜幹細胞を用いた低侵襲な軟骨再生治療のブタでの検討 (2010)
  • Author(s): 中村智祐
  • Organizer: 第14回日本適応医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-03
• [Presentation] Arthroscopic and histological analyses of low invasive articularcartilage repair with porcine synovial mesenchymal stem cells (2010)
  • Author(s): 中村智祐
  • Organizer: 第23回日本軟骨代謝学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-04-03
• [Presentation] スポーツ医学II 理学療法学科主催特別講義 平成21年12月19日 (2009)
  • Author(s): 宗田大
  • Organizer: トライデントスポーツ医療科学専門学校理学療法学科
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-19
• [Presentation] 膝ACL最前線 (2009)
  • Author(s): 宗田大
  • Organizer: トライデントスポーツ医療科学専門学校理学療法学科特別講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-19
• [Presentation] ACL損傷膝を取り巻く諸問題と解決への模索 (2009)
  • Author(s): 宗田大
  • Organizer: 第26回膝関節フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2009-12-05
• [Presentation] 私たちが行ってきた膝靭帯手術 (2009)
  • Author(s): 宗田大
  • Organizer: 第16回中越整形外科手術手技講演会
  • Place of Presentation: 長岡
  • Year and Date: 2009-11-26
• [Presentation] 外来における前十字靭帯損傷の診断と治療 (2009)
  • Author(s): 宗田大
  • Organizer: 埼玉県南スポーツフォーラム
  • Place of Presentation: 埼玉
  • Year and Date: 2009-10-29
• [Presentation] Technical aspects of ACL augmentation. Session5 Anatomical Approach to ACL. (2009)
  • Author(s): 宗田大
  • Organizer: International ATOS LIVE Summit 2009
  • Place of Presentation: Heidelberg
  • Year and Date: 2009-09-25
• [Presentation] 膝の痛みに対するヒアルロン酸治療 (2009)
  • Author(s): 宗田大
  • Organizer: ヒアルロン酸関節療法の臨床と基礎研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-18
• [Presentation] 前十字靭帯損傷と膝のリハビリテーション (2009)
  • Author(s): 宗田大
  • Organizer: H21年度第2回香川県臨床整形外科医会研修会
  • Place of Presentation: 高松
  • Year and Date: 2009-09-04
• [Presentation] ACL再建(ハムストリング) (2009)
  • Author(s): 宗田大
  • Organizer: JOSKASセミナー
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-27
• [Presentation] :多重折り半腱様筋腱を用いた2重束再ACL再建術。シンポジウム4ACL再建術の成績向上を目指して (2009)
  • Author(s): 宗田大, 関矢一郎, 朱寧進, 森戸俊行, 柳下和慶
  • Organizer: JOSKAS2009
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-26
• [Presentation] 人工関節置換術後の血栓予防とクレキサンの使用 (2009)
  • Author(s): 宗田大
  • Organizer: クレキサン発売1周年記念講演会
  • Place of Presentation: 東京
  • Year and Date: 2009-06-06
• [Presentation] 前十字靭帯損傷の診断と治療。教育研修講演42 (2009)
  • Author(s): 宗田大
  • Organizer: 日本整形外科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2009-05-17
• [Presentation] :JOAシンポジウム5 変形性膝関節症の保存療法-エビデンスの構築をめざして-積極的可動域訓練の効果 (2009)
  • Author(s): 宗田大, 山賀美芽, 朱寧進, 森戸俊行, 山崎順也, 関矢一郎, 四宮謙一
  • Organizer: 日本整形外科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2009-05-14
• [Presentation] ICL #12. Technical Considerations in Double Bundle ACL Reconstruction Transtibial approach in figure-four position using moving-a-wire technique (2009)
  • Author(s): 宗田大
  • Organizer: ISAKOS
  • Place of Presentation: Osaka
  • Year and Date: 2009-04-07
• [Presentation] Symposium : Double bundle ACL reconstruction : Any evidence based clinical advantages? Clinical evidence from the literature (2009)
  • Author(s): 宗田大
  • Organizer: ISAKOS
  • Place of Presentation: Osaka
  • Year and Date: 2009-04-05
• [Book] 滑膜間葉幹細胞を使った軟骨再生 (2012)
  • Author(s): 関矢一郎、宗田大
  • Publisher: 朝倉書店
• [Book] 滑膜間葉幹細胞の役割と低侵襲な軟骨再生への応用 (2012)
  • Author(s): 関矢一郎、宗田大
  • Publisher: 整形トピックス
• [Book] 変形性膝関節症をめぐる進歩.滑膜由来の幹細胞による再生医療 (2012)
  • Author(s): 関矢一郎、宗田大
  • Publisher: Bone Joint Nerve
• [Book] 基礎研究から臨床への展開に向けて.滑膜幹細胞を用いた関節軟骨再生治療 (2011)
  • Author(s): 関矢一郎、宗田大
• [Book] 滑膜間葉幹細胞を用いた関節軟骨再生 (2011)
  • Author(s): 関矢一郎
  • Publisher: クリニカルカルシウム
• [Book] 軟骨再生の概要と応用の可能性 (2011)
  • Author(s): 関矢一郎
  • Publisher: 医事新報
• [Book] 半月板の再生医療 (2011)
  • Author(s): 関矢一郎・宗田大
  • Publisher: 臨床整形外科
• [Book] 日常診療に役立つ整形外科領域の痛みの知識。整形・災害外科4月臨時増刊号 (2009)
  • Author(s): 宗田大
  • Total Pages: 542
  • Publisher: 膝部痛点ストレッチ
• [Remarks]
  • URL: http://www.tmd.ac.jp/grad/orj/orj-J.htm
• [Remarks]
  • URL: http://www.tmd.ac.jp/grad/orj/report/index.html
• [Patent(Industrial Property Rights)] 滑膜由来間葉幹細胞の軟骨・半月板再生への応用 (2009)
  • Inventor(s): 関矢一郎, 宗田大, ほか3名
  • Industrial Property Rights Holder: 東京医科産科大学
  • Filing Date: 2009-02-23