Clarifying the role of IKK. NFκB signaling axis in bone formation using lineage specific conditional knockout mice.

• Project/Area Number: 21591941
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University
• Principal Investigator: KATO Tomohisa 京都大学, 再生医科学研究所, 講師 (50301247)
• Co-Investigator(Kenkyū-buntansha): TOGUCHIDA Junya 京都大学, 再生医科学研究所, 教授 (40273502)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 造骨機構 / 細胞系譜 / シグナル伝達 / NF-κB / IKK

Research Abstract

Using osteoblastic cell lineage-specific and chondrocyte specific conditional knockout mice, we have investigated the role of IKK. NF-κB signaling axis in cells that contribute to bone formation. We found that Ikkβplays role in mesenchymal cells of limb bud in early development. Those cells deregulated the chondrocytes in growth plate of the long bone of post natal mice.

Research Products

• [Journal Article] Nuclear IKK(R) is an adaptor protein for I. Bαubiquitination and degradation in UV-induced NF-B activation (2012)
  • Author(s): Tsuchiya, Y., Asano, T., Nakayama, K., Kato, Jr., T., Karin, M. and Kamata, H.
  • Journal Title: Molecular Cell Volume: 39 Pages: 570-582
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells cultured under hypoxia escape from senescence via down-regulation of p16 and extracellular signal regulated kinase (2010)
  • Author(s): Jin, Y., Kato, T., Furu, M., Nasu, A., Kajita, Y., Mitsui, H., Ueda, M., Aoyama, T., Nakayama, T., Nakamura, T. and Toguchida, J.
  • Journal Title: Biochem Biophys Res Commun Volume: 391 Pages: 1471-1476
  • Peer Reviewed
• [Journal Article] Nuclear IKKβ Is an Adaptor Protein for I_κB_α Ubiquitination and Degradation in UV-Induced NF-_κB Activation (2010)
  • Author(s): Tsuchiya, Y, et al
  • Journal Title: Molecular Cell Volume: 39 Pages: 570-582
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells cultured under hypoxia escape from senescence viadown-regulation of p16 and extracellular signal regulated kinase (2010)
  • Author(s): Jin, Y., et al.
  • Journal Title: Biochemical and Biophysical Research Communications 391 Pages: 1471-1476
  • Peer Reviewed
• [Presentation] Epigenetic regulation of FZD10 by SYT-SSX fusion oncogene during the lineage commitment (2011)
  • Author(s): Tamaki S, et al
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-16
• [Presentation] Role for IKK/NF-κB signaling axis during the cellular reprogramming (2011)
  • Author(s): 加藤友久, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-13
• [Presentation] SYT-SSXのエピゲノム発現制御機構への関与 (2011)
  • Author(s): 玉置さくら, 他
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2011-10-03
• [Presentation] SYT-SSXのエピゲノム発現制御機構への関与 (2011)
  • Author(s): 玉置さくら, 他
  • Organizer: 第44回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2011-07-14
• [Presentation] Genetic and Epigenetic Regulation of a Synovial Sarcoma-Associated Molecule, FZD10 (2010)
  • Author(s): 玉置さくら, 他
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Genetic and Epigenetic Regulation of a Synovial Sarcoma-Associated Molecule, FZD10 (2010)
  • Author(s): 玉置さくら, 他
  • Organizer: CTOS 16^<th> Annual Meeting
  • Place of Presentation: パリ、フランス
  • Year and Date: 2010-11-11
• [Presentation] Regulation of a Synovial Sarcoma-Associated Molecule, FZD10 (2010)
  • Author(s): 玉置さくら, 他
  • Organizer: 第43回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-16
• [Presentation] Oncogenic regulation of FZD10 in synovial sarcoma (2009)
  • Author(s): 玉置さくら, 他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09