Identification of osteoblast function regulating factor secreted by human mesenchymal stem cell

• Project/Area Number: 21591945
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: SASAKI Junzo 岡山大学, 大学院・医歯薬学総合研究科, 教授 (30093686)
• Co-Investigator(Kenkyū-buntansha): OZAKI Toshifumi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (40294459) ; FUJITA Hirofumi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (20423288) ; MIKI Yukari 香川県立保健医療大学, 保健医療学部, 助教 (70397876) ; MORIMOTO Yuki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00452574)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 間葉系幹細胞 / 骨芽細胞分化 / 死細胞

Research Abstract

We investigated whether cell death is induced during the calcification process by osteoblast derived from human bone marrow mesenchymal stem cells in vitro, and whether dead cells are involved in the calcification process. Our findings showed that cell death was induced in osteogenic culture of mesenchymal stem cells in vitro, and suggested that caspase-independent dead cells and/or necrotic dead cells of osteoblast lineage might be involved in the mechanism of bone calcification.

Research Products

• [Journal Article] Interferon-beta, but not tumor necrosis factor-alpha, production in response to poly I : C is maintained despite exhaustive exercise in mice (2012)
  • Author(s): Uchida M, Oyanagi E, Kremenik MJ, Sasaki J, Yano H
  • Journal Title: The Journal of Physiological Sciences Volume: 62 Issue: 1 Pages: 59-62
  • DOI: 10.1007/s12576-011-0177-4
  • Peer Reviewed
• [Journal Article] Serum-dependent export of protoporphyrin IX by ATP-binding cassette transporter G2 in T24 cells (2011)
  • Author(s): Ogino T, Kobuchi H, Munetomo K, Fujita H, Yamamoto M, Utsumi T, Inoue K, Shuin T, Sasaki J, Inoue M, Utsumi K.
  • Journal Title: Mol Cell Biochem. Volume: 358(1-2) Pages: 297-307
  • Peer Reviewed
• [Journal Article] Protective action of L-carnitine on cardiac mitochondrial function and structure against fatty acid stress (2011)
  • Author(s): Oyanagi E, Yano H, Uchida M, Utsumi K, Sasaki J.
  • Journal Title: Biochem Biophys Res Commun. Volume: 412(1) Pages: 61-7
  • Peer Reviewed
• [Journal Article] Effect of risedronate on osteoblast differentiation, expression of receptor activator of NF-κB ligand and apoptosis in mesenchymal stem cells (2011)
  • Author(s): Fujita H, Kurokawa K, Ogino T, Ono M, Yamamoto M, Oka T, Nakanishi T, Kobayashi N, Tanaka N, Ogawa T, Suzaki E, Utsumi K, Sasaki J.
  • Journal Title: Basic Clin Pharmacol Toxicol. Volume: 109(2) Issue: 2 Pages: 78-84
  • DOI: 10.1111/j.1742-7843.2011.00685.x
  • Peer Reviewed
• [Journal Article] Protective action of L-carnitine on cardiac mitochondrial function and structure against fatty acid stress (2011)
  • Author(s): Oyanagi E, Yano H, Uchida M, Utsumi K, Sasaki J
  • Journal Title: Biochem Biophys Res Commun Volume: 412 Pages: 61-67
  • Peer Reviewed
• [Journal Article] Serum-dependent export of protoporphyrin IX by ATP-binding cassette transporter G2 in T24 cells (2011)
  • Author(s): Ogino T, Kobuchi H, Munetomo K, Fujita H, Yamamoto M, Utsumi T, Inoue K, Shuin T, Sasaki J, Inoue M, Utsumi K
  • Journal Title: Mol Cell Biochem Volume: 358 Pages: 297-307
  • Peer Reviewed
• [Journal Article] Effect of Risedronate on Osteoblast Differentiation, Expression of Receptor Activator of NF-κB Ligand and Apoptosis in Mesenchymal Stem Cells. (2011)
  • Author(s): Fujita H, Sasaki J, et al
  • Journal Title: Basic Clin Pharmacol Toxicol Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] α-Tocopheryl succinate induces rapid and reversible phosphatidylserine externalization in histiocytic lymphoma through the caspase-independent pathway (2010)
  • Author(s): Fujita H, Shiva D, Utsumi T, Ogino T, Ogawa T, Abe K, Yasuda T, Utsumi K and Sasaki J.
  • Journal Title: Mol Cell Biochem. Volume: 333 Pages: 137-149
  • Peer Reviewed
• [Journal Article] α-Tocopheryl succinate induces rapid and reversible phosphatidylserine externalization in histiocytic lymphoma through the caspase-independent pathway (2010)
  • Author(s): Fujita H, Sasaki J, et al
  • Journal Title: Mol Cell Biochem Volume: 333 Pages: 137-149
  • Peer Reviewed
• [Journal Article] ビタミンEサクシネートによるホスファチジルセリンの細胞表面転移の解析 (2010)
  • Author(s): 藤田洋史, 佐々木順造, ら
  • Journal Title: ビタミンE研究の進歩XIV Volume: XIV Pages: 13-20
• [Journal Article] α-tocopheryl succinate induces rapid and reversible phosphatidylserine externalization in histiocytic lymphoma through the caspase-independent pathway (2010)
  • Author(s): Fujita H, Sasaki J, et al
  • Journal Title: Mol Cell Biochem 333 Pages: 137-149
  • Peer Reviewed
• [Journal Article] Regulation of 5-amtnolevulinic Acid-mediated Protoporphyrin IX-accumulation in Human Urothelial Carcinomas. (2009)
  • Author(s): Inoue K, Sasaki J, et. Al
  • Journal Title: Pathobiology 76 Pages: 303-314
  • Peer Reviewed
• [Presentation] 骨髄間葉系幹細胞の骨芽細胞分化誘導に伴う細胞死と石灰化の機構解析 (2011)
  • Author(s): 藤田洋史、内海耕慥、佐々木順造
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Effect ofα-tocopherol and its derivative in TNFα-induced osteoclastogenesis in vitro (2011)
  • Author(s): Hirofumi Fujita, Junzo Sasaki, et al
  • Organizer: The 5th Biennial Meeting of Society for Free Radical Research-Asia(SFRR-Asia)
  • Place of Presentation: Kagoshima
• [Presentation] In vitroにおける骨髄間葉系幹細胞の骨芽細胞分化に伴う石灰化には細胞死が関与する (2011)
  • Author(s): 藤田洋史、斧美緒、佐々木順造
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] 炎症性サイトカインによる破骨細胞形成機構とそれに対するビタミンE誘導体の影響 (2011)
  • Author(s): 藤田洋史、佐々木順造
  • Organizer: 第64回日本酸化ストレス学会
  • Place of Presentation: 北海道
• [Presentation] Dead cells promote calcification of mesenchymal stem cell-derived osteoblasts in vitro (2011)
  • Author(s): Hirofumi Fujita, Junzo Sasaki, et al.
  • Organizer: 第116回日本解剖学会総会・全国学術集会
  • Place of Presentation: 横浜
• [Presentation] Effect of α-tocopherol and its derivative in TNFα-induced osteoclastogenesis in vitro (2011)
  • Author(s): Hirofumi Fujita, Tetsuya Ogino, Mio Ono, Toshio Kukita, Kozo Utsumi, Junzo Sasaki
  • Organizer: The 5th Biennial Meeting of Society for Free Radical Research-Asia
  • Place of Presentation: Kagoshima
• [Presentation] Dead cells promote calcification of mesenchymal stem cell-derived osteoblasts in vitro (2011)
  • Author(s): Fujita H, Sasaki J, et al
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 横浜
• [Presentation] 間葉系幹細胞の骨芽細胞分化に伴う石灰化における細胞死の関与 (2010)
  • Author(s): 藤田洋史、佐々木順造
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] INHIBITION OF ATP-BINDING CASSETTE TRANSPORTER G2 INCREASED MITOCHONDRIAL LOCALIZATION OF PPIX IN ALA TREATED CELLS AND ENHANCED THEIR APOPTOTIC CELLBY PHOTODYNAMIC TREATMENT (2010)
  • Author(s): Utsumi K., Fujita H, Sasaki J, et al.
  • Organizer: OzBio2010 Combined Conference
  • Place of Presentation: Melbourne
• [Presentation] ヒト間葉系幹細胞による炎症性破骨細胞分化の制御とビスフォスフォネートの影響. The suppression of inflammatory stimuli-induced osteoclast differentiation by human mesenchymal stem cell and the action of bisphosphonate (2010)
  • Author(s): 斧美緒、藤田洋史, 佐々木順造
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] 5-Aminolevulinic acid依存性protoporphyrin IX蓄積に於けるABC transporterの機能と光動力学作用 (2010)
  • Author(s): 藤田洋史、佐々木順造、内海耕慥
  • Organizer: 第63回日本酸化ストレス学会学術集会
  • Place of Presentation: 神奈川
• [Presentation] 間葉系幹細胞における骨粗鬆症治療薬リセドロネートの骨芽細胞分化とRANKL発現への影響 (2010)
  • Author(s): 藤田洋史、佐々木順造
  • Organizer: 第115回日本解剖学会総会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] 間葉系幹細胞の骨芽細胞分化に伴う石灰化における細胞死の関与 (2010)
  • Author(s): 藤田洋史, 佐々木順造, ら
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Presentation] αトコフェロールサクシネートによる細胞膜ホスファチジルセリンの細胞表面転移作用と細胞死の機構 (2009)
  • Author(s): 藤田洋史、佐々木順造、内海耕慥
  • Organizer: 第12回Vitamin E Update Forum
  • Place of Presentation: 東京
  • Year and Date: 2009-08-31
• [Presentation] 骨粗鬆症治療薬リセドロネートの間葉系幹細胞に対する作用-骨芽細胞分化を中心として- (2009)
  • Author(s): 黒川佳津子, 藤田洋史, 佐々木順造
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
• [Presentation] アポトーシスにおける活性型カスパーゼ3と細胞骨格構造との結合. Active form of caspase-3 binds to actin cytoskeletal structure in apoptosis (2009)
  • Author(s): 藤田洋史、佐々木順造
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
• [Presentation] 5-アミノレブリン酸依存性プロトポルフィリンIXを利用した癌細胞イメージングとその蓄積機構の解析 (2009)
  • Author(s): 藤田洋史、佐々木順造
  • Organizer: 第18回バイオイメージング学術集会
  • Place of Presentation: 岡山
• [Presentation] Involvement of caspase-dependent and-independent cell death pathways in 5-ALA-mediated photodynamic treatment in human histiocytic lymphoma cell line U937 (2009)
  • Author(s): Kozo Utsumi, Junzo Sasaki, et al
  • Organizer: 21st IUBMB International Congress
  • Place of Presentation: Shanghai, China
• [Presentation] PC12細胞における6-Hydroxydopamine誘導性アポトーシスには細胞内鉄イオンが関与する (2009)
  • Author(s): 藤田洋史、佐々木順造
  • Organizer: 第62回日本酸化ストレス学会学術集会
  • Place of Presentation: 福岡