Local mechanism of new bone formatior in bone grafting-interaotion between cells in the graft and cells in host-
| Project/Area Number |
21591954
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka City University |
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Principal Investigator |
NAKAMURA Hiroaki 大阪市立大学, 大学院・医学研究科, 教授 (60227931)
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| Co-Investigator(Kenkyū-buntansha) |
TERAI Hidetomi 大阪市立大学, 大学院・医学研究科, 講師 (20382046)
TOYODA Hiromitsu 大阪市立大学, 大学院・医学研究科, 病院講師 (50514238)
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| Co-Investigator(Renkei-kenkyūsha) |
YANO Koichi 大阪労災病院, 整形外科
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 自家骨移植 / 細胞の起源 / Green Fluorescent Protein / 骨形成蛋白 / 骨芽細胞 / 破骨細胞 / 新生骨 / 骨形成タンパク |
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| Research Abstract |
Although autogenous bone grafting is currently available treatment. local mechanism of new bone formation has riot been analyzed clearly. The aim of this study was to examine the relationship between cells in the graft and host for new bone formation. We used GFP transgenic rats and wild-type rats, and ectopic isograft model that harvested femur was transplanted into back muscles between these kinds of rats was established. Gratis were divided into two groups, without treatment(experimental group) and treated by liquid nitrogen(control croup). Specimens were examined by immunohistochemistry for origin of osteoblast and osteoclast, real time RT-PCR, and in situ hybridization for temporal and spatial expression of BMP signaling. Eclopic new bone formation was found in the medullary canal at day 7 after grafting, and remodeling was followed in experimental group. No bone formation was found in control group. At day 7, osteoblasts were mainly originated from graft. Osteoblasts originated from host increased at day 14.and almost all of the osteoblasts were replaced by host at day 42.Transient increase of BMP-4.BMPRIA, 2, and noggin mRNA was found in experimental group, but only BMPRIA and 2 mRNA in control group. BMP-4 mRNA was expressed in graft cells at day 7 and in both graft and host cells at day 14.Collectively our results indicated the possibility for the First time that cells in graft induced host cells to bone formation by expressing BMP-4 mRNA.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Prognostic Factors for Reduction of Activities of Da ily Living Following Osteoporotic Vertebral Fractures2011
Author(s)
Matsumoto T, Hoshino M, Tsujio T, Terai H, Namikawa T, Matsumura A, Kato M, Tovoda H, Suzuki A, Takayama K, Takaoka K, Nakamura H
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Journal Title
NAID
Related Report
Peer Reviewed
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[Journal Article] Characteristic radiographic or magnetic resonance images of fresh osteoporotic vertebral fractures predicting potential risk for nonunion : a prospective multicenter study2011
Author(s)
Tsujio T, Nakamura H, Terai H, Hoshino M, Namikawa T, Matsumura A, Kato M, Suzuki A, Takayama K, Fukushima W, Kondo K, Hirota Y, Takaoka K
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Journal Title
Spine(Phila Pa 1976)
Volume: 36(15)
Pages: 1229-35
NAID
Related Report
Peer Reviewed
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[Presentation] The prevalence of diffuse idiopathic skeletal hyperostosis(DISH) in the patients with spinal disorders2011
Author(s)
H Toyoda, H Terai, K Yamada, A Suzuki, S Dozono, T Matsumoto, H Yasuda, S Takahashi, K Tsukiyama, H Nakamura
Organizer
International Society for the Study of the Lumbar Spine 39th Annual Meeting
Place of Presentation
Gurthenberg, Sweden
Related Report
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[Presentation] The prevalence of diffuse idiopathic skeletal hyperostosis(DISH) in the patients with spinal disorders2011
Author(s)
H Toyoda, H Terai, K Yamada, A Suzuki, S Dozono, T Matsumoto, H Yasuda, S Takahashi, K Tsukiyama, H Nakamura
Organizer
The 8th Combined Congress of the Spine and Pediatric Sections, Asia Pacific Orthopaedic Association
Place of Presentation
Gifu city. Japan
Related Report
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[Presentation] The prevalence of diffuse idiopathic skeletal hyperostosis(DISH) in the patients with spinal disorders2011
Author(s)
H Tovoda, H Terai, K Yamada, A Suzuki, S Dozono, T Matsumoto, H Yasuda, S Takahashi, K Tsukiyama, H Nakamura
Organizer
The 18th International Meeting on Advanced Spine Techniques
Place of Presentation
Copenhagen, Denmark
Related Report
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