| Project/Area Number |
21591970
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
IIDA Hiroki 岐阜大学, 医学系研究科, 教授 (30159561)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IIDA Mami 岐阜大学, 医学系研究科, 非常勤講師 (80350859)
土肥 修司 岐阜大学, 大学院・医学系研究科, 教授 (80136952)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 性ホルモン / エストロジェン / リモートプレコンディショニング / 脊髄虚血 / 性差 / 性ステロイド / 対麻痺 / 脊髄保護 |
|---|
| Research Abstract |
We evaluated the tissue oxygen partial pressure of spinal cord(PsptO2) following aortic cross-clamping and-unclamping, and then clarified the mechanism of improvement in the PsptO2 following spinal ischemia observed with remote ischemic preconditioning(RIPC) or intravenous estrogen in rabbits. In isoflurane anesthetized rabbits(n=36), we prepared for measurement of PsptO2 following aortic cross-clamping using tissue PO2 monitoring. In the first set of experiment, we allocated 18 animals into 3 groups,(a) control group ; n=6,(b) RIPC group ; n=6,(c) glibenclamide pretreatment(0. 3mg/kg) group ; n=6. In the second set of experiment, we also allocated 18 animals into 3 groups,(d) control group ; n=6,(e) estrogen(17ss estradiol : 20μg) group ; n=6,(f) fulvestrant pretreatment(1mg/kg) group ; n=6. RIPC improved deterioration of the values in PsptO2 at 20 min after aortic cross-clamping and 0, 2, 5 min after aortic unclamp. The improvement was diminished by pretreatment of glibenclamide. Administration of 17s estradiol improved deterioration of the values in PsptO2 at 20 min after aortic cross-clamping and 0, 2, 5 min after aortic unclamp. The improvement was diminished by pretreatment of fulvestrant.
In conclusion, after aortic cross-clamping, the RIPC performed improves the deterioration in PsptO2 through the KATP channel activation and 17ss estradiol improves the deterioration in PsptO2 through the estrogen receptor activation.
|
