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The mechanisms of lidocaine induce apoptosis in cancer cells

Research Project

Project/Area Number 21591978
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionUniversity of Miyazaki

Principal Investigator

ONIZUKA Shin  宮崎大学, 医学部, 助教 (20264393)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords麻酔学 / 局所麻酔薬 / リドカイン / ミトコンドリア / アポトーシス / 腫瘍細胞 / アルカリ化 / 腫瘍特異性 / 細胞内ATP濃度
Research Abstract

BACKGROUND : Depolarization of the mitochondrial membrane potential(DeltaPsim), one of the markers of mitochondrial failure, is regulated by the proton electrochemical gradient(Delta H(+)). Therefore, intracellular pH([pH] in) and mitochondrial pH([pH] m) are important factors for modifying DeltaPsim. However, the effects of local anesthetics on [pH] in and[pH] m are unclear. To investigate mitochondrial responses to local anesthetics, we simultaneously measured [pH] m and [pH] in, along with mitochondrial membrane potential
Our study demonstrated that uncharged(base) forms of local anesthetics induce mitochondrial membrane potential depolarization. One of the causes is intracellular and mitochondrial alkalization.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (17 results)

All 2012 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (5 results)

  • [Journal Article] Clinical dose of lidocaine destroys cell membrane and induces both necrosis and apoptos is inan identified Lymnaea neuron2012

    • Author(s)
      Onizuka S, Tamura R, Yonaha T, Oda N, Kawasaki Y, Shirasaka T, Shiraishi S, and Tsuneyoshi I
    • Journal Title

      J Anesth

      Volume: 26 Pages: 54-61

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lidocaine treatment during synapse reformation periods permanently inhibits NGF-induced excitation in an identified reconstructed synapse of Lymnaea stagnalis2012

    • Author(s)
      Onizuka S, Shiraishi S, Tamura R, YonahaT, Oda N, Kawasaki Y, Syed NI, ShirasakaT, and Tsuneyoshi I
    • Journal Title

      J Anesth

      Volume: 26 Pages: 45-53

    • NAID

      10030449380

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Capsaicin indirectly suppresses voltage-gated Na+ currents through TRPV1 in rat dorsal root ganglion neurons2011

    • Author(s)
      Onizuka S, Yonaha T, Tamura R, HosokawaN, Kawasaki Y, Kashiwada M, Shirasaka T, Tsuneyoshi I
    • Journal Title

      Anesth Analg

      Volume: 112 Pages: 703-9

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lidocaine depolarizes the mitochondrial membrane potential by intracellular alkalization in rat dorsal root ganglion neurons2011

    • Author(s)
      Onizuka S, Yonaha T, Tamura R, Kasiwada M, Shirasaka T, Tsuneyoshi I
    • Journal Title

      J Anesth

      Volume: 25 Pages: 229-39

    • NAID

      10029022520

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Local Anesthetics with High Lipophilicity are Toxic, While Local Anesthetics with Low pka Induce More Apoptosis in Human Leukemia Cells2011

    • Author(s)
      Onizuka S, YonahaT, TsuneyoshiI
    • Journal Title

      J Anesthe Clinic Res

      Volume: 2 Pages: 116-21

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Capsaicin indirectly suppresses voltage-gated Na+ currents through TRPV1 in rat dorsal root ganglion neurons2011

    • Author(s)
      Onizuka Shin
    • Journal Title

      nesthesia and analgesia

      Volume: 112 Pages: 703-709

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Local Anesthetics with High Lipophilicity are Toxic, While Local Anesthetics with Low pka Induce More Apoptosis in Human Leukemia Cells2011

    • Author(s)
      Onizuka Shin
    • Journal Title

      Anesthesia and Clinical Research

      Volume: 2 Pages: 116-121

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lidocaine depolarizes the mitochondrial membrane potential by intracellular alkalization in rat dorsal root ganglion neurons2011

    • Author(s)
      Onizuka Shin
    • Journal Title

      Journal of anesthesia

      Volume: 25 Pages: 229-239

    • NAID

      10029022520

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lidocaine depolarizes the mitochondrial membrane potential by intracellular alkalization in rat dorsal root ganglion neurons.2011

    • Author(s)
      Onizuka S, Yonaha T, Tamura R, Kasiwada M, Shirasaka T, Tsuneyoshi I.
    • Journal Title

      J Anesth.

      Volume: 25 Pages: 229-239

    • NAID

      10029022520

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Local anesthetics depolarize mitochondrial membrane potential by intracellular alkalization in rat dorsal root ganglion neurons.2011

    • Author(s)
      Onizuka S, Tamura R, Hosokawa N, Kawasaki Y, Tsuneyoshi I.
    • Journal Title

      Anesth Analg.

      Volume: 111 Pages: 775-783

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Local Anesthetics with High Lipophilicity are Toxic, While Local Anesthetics with Low pka Induce More Apoptosis in Human Leukemia Cells2011

    • Author(s)
      Shin Onizuka, Tetsu Yonaha, Isao Tsuneyoshi
    • Journal Title

      J Anesthe Clinic Res.

      Volume: 21 Pages: 11-16

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Local anesthetics depolarize mitochondrial membrane potential by intracellular alkalization in rat dorsal root ganglion neurons2010

    • Author(s)
      Onizuka S, Tamura R, Hosokawa N, Kawasaki Y, Tsuneyoshi I
    • Journal Title

      Anesthesia and analgesia

      Volume: 111 Pages: 775-83

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] 培養腫瘍細胞からの腫瘍致死因子(TNF-α)の分泌2011

    • Author(s)
      鬼塚信, 田中信彦, 與那覇哲, 田村隆二, 恒吉勇男
    • Organizer
      日本麻酔科学会第58回学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2011-05-19
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 腫瘍細胞分泌液は,ラット脊髄神経節ニューロンにおいて電位依存性ナトリウムチャネルNaV1. 3の発現量を増加する2010

    • Author(s)
      鬼塚信, 田中信彦, 與那覇哲, 田村隆二, 恒吉勇男
    • Organizer
      九州麻酔科学会第48回大会
    • Place of Presentation
      福岡
    • Year and Date
      2010-09-25
    • Related Report
      2011 Final Research Report
  • [Presentation] リドカインによるミトコンドリア経路のアポトーシス誘発について:腫瘍細胞を用いた検討2010

    • Author(s)
      鬼塚信, 山下幸貴, 田村隆二, 恒吉勇男
    • Organizer
      日本麻酔科学会第57回学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2010-06-03
    • Related Report
      2011 Final Research Report
  • [Presentation] リドカインによるミトコンドリア経路のアポトロシス誘発について:腫瘍細胞を用いた検討2010

    • Author(s)
      鬼塚信、山下幸貴、田村隆二、恒吉勇男
    • Organizer
      日本麻酵科学会第57回学術集会
    • Place of Presentation
      福岡市
    • Year and Date
      2010-06-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] リドカインはヒト白血病細胞にアポトーシスとネクローシスそれぞれを誘発する2009

    • Author(s)
      鬼塚信, 山下幸貴, 田村隆二, 恒吉勇男
    • Organizer
      日本麻酔科学会第56回学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2009-08-18
    • Related Report
      2011 Final Research Report 2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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