| Project/Area Number |
21591978
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | University of Miyazaki |
|---|
Principal Investigator |
ONIZUKA Shin 宮崎大学, 医学部, 助教 (20264393)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
|---|
| Keywords | 麻酔学 / 局所麻酔薬 / リドカイン / ミトコンドリア / アポトーシス / 腫瘍細胞 / アルカリ化 / 腫瘍特異性 / 細胞内ATP濃度 |
|---|
| Research Abstract |
BACKGROUND : Depolarization of the mitochondrial membrane potential(DeltaPsim), one of the markers of mitochondrial failure, is regulated by the proton electrochemical gradient(Delta H(+)). Therefore, intracellular pH([pH] in) and mitochondrial pH([pH] m) are important factors for modifying DeltaPsim. However, the effects of local anesthetics on [pH] in and[pH] m are unclear. To investigate mitochondrial responses to local anesthetics, we simultaneously measured [pH] m and [pH] in, along with mitochondrial membrane potential
Our study demonstrated that uncharged(base) forms of local anesthetics induce mitochondrial membrane potential depolarization. One of the causes is intracellular and mitochondrial alkalization.
|
