Functional analysis of water channel for mild hypothermia-induced brain edema decrease using knockdown technique
| Project/Area Number |
21591982
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Nagoya City University |
|---|
Principal Investigator |
FUJITA Yoshihito 名古屋市立大学, 大学院・医学研究科, 講師 (90238593)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ASAI Kiyofumi 名古屋市立大学, 大学院・医学研究科, 教授 (70212462)
SOBUE Kazuya 名古屋市立大学, 大学院・医学研究科, 教授 (90264738)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 水チャンネル / 脳浮腫 / ノックダウン / アクアポリン / 脳低温 / RANi / RNAi |
|---|
| Research Abstract |
We constructed hAQP4-M23 and hAQP4-M1 vector and transfected into C6 cells. We confirmed the overexpression of hAQP4-M1 and hAQP4-M23 in C6 cells. Moreover, we constructed permanent cell line of hAQP4-M23-overexpressed-cells of C6.We constructed vector of knockdown for AQP4, however, we could not obtain efficacy of knockdown for AQP4 using our constructed vectors. We tried a new method using lentiviral vectors
|
Report
(4 results)
Research Products
(17 results)
