The approach to a trigeminal neuralgia mechanism based on a local GABA system hypothesis

• Project/Area Number: 21591990
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Osaka Medical College
• Principal Investigator: HAYASAKI Hana 大阪医科大学, 医学部, 助教 (90257866)
• Co-Investigator(Kenkyū-buntansha): SOHAMA Yoshiro 慶應義塾大学, 医学部, 准教授 (60268183) ; FUKUDA Atsuo 浜松医科大学, 医学部, 教授 (50254272)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: GABA / 三叉神経節 / satellite cell / GABAB受容体 / KCTD12 / KCTD16 / グリア細胞 / 神経伝達物質 / release / 膜電位 / baclofen / Ca2+イメージング / Na channel

Research Abstract

We investigated possibility of the neural transmission regulation of the SC-nerve cell by imaging of extracellular GABA release at the trigeminal ganglion.
As a result, GABA was released both from neuron and SG on dispersion culture, and the GABA release of neuron was inhibited by GAT inhibitor(nipecotic acid), and GABA release of SG was inhibited by anion channel inhibitor(DNDS).
As a result, GABA was released both from neuron and SG on dispersion culture, and the GABA release of neuron was inhibited by GAT inhibitor(nipecotic acid), and GABA release of SG was inhibited by anion channel inhibitor(DNDS).

Research Products

• [Journal Article] A hyaluronan synthase suppressor, 4-methylumbelliferone, inhibits the tumor invasion associated with N-cadherin decreasement (2011)
  • Author(s): Kuwabara H, Yoneda M, Hayasaki H, Nakamura T, Shibayama Y
  • Journal Title: Pathol Int Volume: 61(4) Issue: 4 Pages: 262-3
  • DOI: 10.1111/j.1440-1827.2011.02660.x
  • NAID: 10031185082
  • Peer Reviewed
• [Journal Article] KCC2 was downregulated in small neurons localized in epileptogenic human focal cortical dysplasia (2011)
  • Author(s): Shimizu-Okabe, C., Tanaka, M., Matsuda, K., Mihara, T., Okabe, A., Sato, K., Inoue, Y., Fujiwara, T., Yagi, K., Fukuda, A
  • Journal Title: Epilepsy Res Volume: 93 Pages: 177-184
  • Peer Reviewed
• [Journal Article] A heterozygous deletion in the glutamate decarboxylase 67 gene enhances maternal and fetal stress vulnerability (2011)
  • Author(s): Uchida, T., Oki, Y., Yanagawa, Y. and Fukuda A
  • Journal Title: Neurosci. Res Volume: 69 Pages: 276-282
  • Peer Reviewed
• [Journal Article] Curcumin and genistein additively potentiate G551D-CFTR (2011)
  • Author(s): Yu YC, Miki H, Nakamura Y, Hanyuda A, Matsuzaki Y, Abe Y, Yasui M, Tanaka K, Hwang TC, Bompadre SB, Sohma Y
  • Journal Title: J. Cystic Fibrosis Volume: 10 Pages: 243-252
  • Peer Reviewed
• [Journal Article] A stable ATP binding to nucleotide binding domain is important for reliable gating cycle in an ABC transporter CFTR (2010)
  • Author(s): Shimizu H, Yu YC, Kono K, Kubota T, Yasui M, Li M, Hwang TC, Sohma Y
  • Journal Title: J. Physiol. Sci Volume: 60 Pages: 353-362
  • Peer Reviewed
• [Presentation] GABA handling system in the rat trigeminal ganglion (2012)
  • Author(s): 早崎華
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学
  • Year and Date: 2012-03-27
• [Presentation] GABA handling system in the rat trigeminal ganglion (2012)
  • Author(s): 早崎華, 森島寿貴, 相馬義郎, 吉田祥子, 福田敦夫, 大槻勝紀
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2012-03-27
• [Presentation] An immunocytochemical study for the local GABAergic system in rat trigeminal ganglion (2011)
  • Author(s): 早崎華
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-16
• [Book] Plant Transformation Technologies (2010)
  • Author(s): 渡辺正仁、小西正良、杉本雅晴、西村敦、柴田雅朗、伊藤裕子、早崎華、金山忠志
  • Total Pages: 396
  • Publisher: 廣川書店