Basic research of pain control by DNA decoy for brain-derived neurotrophic factor

• Project/Area Number: 21592008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Kochi University
• Principal Investigator: YOKOYAMA Masataka 高知大学, 教育研究部・医療学系, 教授 (20158380)
• Research Collaborator: OBATA Norihiko 岡山大学, 病院, 助教 ; MATSUOKA Yoshikazu 岡山大学, 病院, 助教
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経障害性痛 / 脳由来神経栄養因子 / エクソン / DNAデコイ / 遺伝子療法 / 疼痛

Research Abstract

We made DNA decoy for brain-derived neurotrophic factor(BDNF) exon-1 that is increased significantly in the spinal level of neuropathic pain model. We administered DNA decoy intrathecally to knock-down BDNF exon-1 in pain model rats, and confirmed that pain response was decreased by DNA decoy.

Research Products

• [Journal Article] Decoy strategy targeting the brain-derived neurotrophic factor exon I to attenuate tactile allodynia in the neuropathic pain mode l of rats (2011)
  • Author(s): Obata N, Mizobuchi S, Yokoyama M, et al
  • Journal Title: Biochem Biophys Res Commun Volume: vol408 Pages: 139-144
  • Peer Reviewed
• [Journal Article] Role of the neurosteroid allopregnanolone in the hyperalgesic behavior induced by painful nerve injury in rats (2011)
  • Author(s): Kawano T, Yokoyama M
  • Journal Title: J Anesth Volume: vol25 Pages: 942-945
  • NAID: 10030449157
  • Peer Reviewed
• [Journal Article] The validation of pin-evoked hyperalgesia-like behavior as a measure of pain in rats (2011)
  • Author(s): Kawano T, Yokoyama M
  • Journal Title: Pain Res Volume: vol26 Pages: 223-227
  • Peer Reviewed
• [Journal Article] The involvement of the neurosteroidallopregnanolone in the anti-hyperalgesic effect of paroxetine in a rat model of neuropathic pain (2011)
  • Author(s): Kawano T, Yokoyama M
  • Journal Title: Neuroreport Volume: vol22 Pages: 984-988
  • Peer Reviewed
• [Journal Article] Decoy strategy targeting the brain-derived neurotrophic factor exon I to attenuate tactile allodynia in the neuropathic pain model of rats (2011)
  • Author(s): Obata N, Yokoyama M, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 408 Issue: 1 Pages: 139-144
  • DOI: 10.1016/j.bbrc.2011.03.137
  • Peer Reviewed
• [Journal Article] Role of the neurosteroid allopregnanolone in the hyperalgesic behavior induced by painful nerve injury in rats (2011)
  • Author(s): Kawano T, Yokoyama M, et al
  • Journal Title: J Anesth Volume: 25 Issue: 6 Pages: 942-945
  • DOI: 10.1007/s00540-011-1216-2
  • NAID: 10030449157
  • Peer Reviewed
• [Journal Article] The validatiom of pin-evoked hyperalgesia-like behavior as a measure of pain in rats (2011)
  • Author(s): Kawano T, Yokoyama M
  • Journal Title: Pain Res Volume: 26 Pages: 223-227
  • Peer Reviewed
• [Journal Article] The involvement of the neurosteroidallopregnanolone in the anti-hyperalgesic effect of paroxetine in a rat model of neuropathic pain (2011)
  • Author(s): Kawano T, Yokoyama M, et al
  • Journal Title: Neuroreport Volume: 22 Issue: 18 Pages: 984-988
  • DOI: 10.1097/wnr.0b013e32834da80d
  • Peer Reviewed
• [Journal Article] The evaluation of the ability of closed-loop glycemic control device to maintain the blood glucose concentration in intensive care unitpatients. (2011)
  • Author(s): Yatabe T, Yamazaki R, Yokoyama M, et al.
  • Journal Title: Crit Care Med Volume: 39 Pages: 604-605
  • Peer Reviewed
• [Journal Article] Decoy strategy targeting the brain-derived neurotrophic factor exon I to attenuate tactile allodynia in the neuropathic pain model of rats. (2011)
  • Author(s): Obata N, Mizobuchi S, Yokoyama M, et al
  • Journal Title: Biochem Biophys Res Commun Volume: (In press)
  • Peer Reviewed
• [Journal Article] Nonimmersive Virtual Reality Mirror Visual Feedback Therapy and Its Application for the Treatment of Complex Regional Pain Syndrome : An Open-Label Pilot Study (2010)
  • Author(s): Sato K, Fukumori S, Yokoyama M, et al
  • Journal Title: Pain Med Volume: vol11 Pages: 622-629
  • Peer Reviewed
• [Journal Article] 麻酔・神経ブロックと免疫・予後 (2010)
  • Author(s): 横山正尚
  • Journal Title: 臨床麻酔 Volume: 34巻 Pages: 799-807
  • Peer Reviewed
• [Journal Article] Safer glycemic control using isomaltulose-based enteral formula : A pilot randomized crossover trial. (2010)
  • Author(s): Egi M, Toda Y, Yokoyama M, et al
  • Journal Title: J Crit Care Volume: 25 Pages: 90-96
  • Peer Reviewed
• [Journal Article] Nonimmersive Virtual Reality Mirror Visual Feedback Therapy and Its Application for the Treatment of Complex Regional Pain Syndrome : An Open-Label Pilot Study. (2010)
  • Author(s): Sato K, Fukumori S, Yokoyama M, et al.
  • Journal Title: Pain Med Volume: 11 Pages: 622-629
  • Peer Reviewed
• [Journal Article] Better postoperative oxygenation in thoracoscopic esophagectomy in prone positioning. (2010)
  • Author(s): T Yatabe, H Kitagawa, M Yokoyama, et al
  • Journal Title: J Anesth Volume: 24 Pages: 803-806
  • NAID: 10027741023
  • Peer Reviewed
• [Journal Article] 抗血小板薬および抗凝固薬と硬膜外麻酔-リスクとベネフィットは的確か? (2010)
  • Author(s): 横山正尚
  • Journal Title: 日臨麻会誌 Volume: 142 Pages: 142-150
  • Peer Reviewed
• [Journal Article] Safer glycemic control using isomaltulose-based enteral formula (2010)
  • Author(s): Egi M, Yokoyama M, et al.
  • Journal Title: J Crit Care 25 Pages: 90-96
  • Peer Reviewed
• [Journal Article] Nonimmersive Virtual Reality Mirror Visual Feedback Therapy and Its Application for the Treatment of Complex Regional Pain Syndrom (2010)
  • Author(s): Sato K, Yokoyama M, et al
  • Journal Title: Pain Med (In press)
  • Peer Reviewed
• [Journal Article] モルヒネIVPCA (2009)
  • Author(s): 横山正尚
  • Journal Title: 日本臨床麻酔学会誌 Volume: 11巻 Pages: 13-22
  • NAID: 10026352679
  • Peer Reviewed
• [Journal Article] 全身麻酔下に硬膜外麻酔を行って良いか (2009)
  • Author(s): 横山正尚
  • Journal Title: LISA Volume: 16巻 Pages: 196-200
  • Peer Reviewed
• [Journal Article] Anesthetic management of a patient with Alport-leiomyomatosis syndrome (2009)
  • Author(s): Hanazaki M, Yokoyama M, et al
  • Journal Title: J Anesth 23 Pages: 453-455
  • NAID: 10027264481
  • Peer Reviewed
• [Journal Article] 全身麻酔下に硬膜外麻酔を行って良いか? (2009)
  • Author(s): 横山正尚
  • Journal Title: LISA 16 Pages: 196-200
  • Peer Reviewed
• [Journal Article] モルヒネIVPCA (2009)
  • Author(s): 横山正尚
  • Journal Title: 日本臨床麻酔学会誌 30 Pages: 13-22
  • NAID: 10026352679
  • Peer Reviewed
• [Journal Article] 疼痛科学の確立とエビデンスに基づく臨床 (2009)
  • Author(s): 横山正尚
  • Journal Title: ペインクリニック 30 Pages: 1301-1302
  • Peer Reviewed
• [Presentation] Anti-Hyperalgesic Effects of the Neurosteroid Allopregnanolone in Neuropathic Rats (2011)
  • Author(s): Maeda Y, Yokoyama M, et al
  • Organizer: American Society of Anesthesiologists2011
  • Place of Presentation: Chicago(USA)
  • Year and Date: 2011-10-15
• [Presentation] Anti-Hyperalgesic Effects of the Neurosteroid Allopregnanolone in Neuropathic Rats (2011)
  • Author(s): Maeda Y, Yokoyama, et al
  • Organizer: Anesthesiology 2011
  • Place of Presentation: Chicago, USA, MacCormick Place
  • Year and Date: 2011-10-15
• [Presentation] 神経障害性疼痛モデルラットの痛覚過敏行動に及ぼす神経ステロイドAllopregnanoloneの効果 (2011)
  • Author(s): 河野崇,横山正尚
  • Organizer: 第33回日本疼痛学会
  • Place of Presentation: 愛媛県民文化会館(松山市)
  • Year and Date: 2011-07-22
• [Presentation] 神経障害性疼痛モデルラットの痛覚過敏行動に及ぼす神経ステロイドAllopregnanoloneの効果 (2011)
  • Author(s): 河野崇, 横山正尚
  • Organizer: 第33回日本疼痛学会
  • Place of Presentation: 松山,愛媛県民文化会館
  • Year and Date: 2011-07-22
• [Presentation] 術後痛に対してNK1受容体拮抗薬アプレピタントは有効か (2011)
  • Author(s): 廣橋美穂,河野崇、横山正尚
  • Organizer: 日本麻酔科学会第58回学術集会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2011-05-19
• [Presentation] von Frey filament刺激による逃避行動は神経障害モデル動物で侵害刺激反応であるか (2011)
  • Author(s): 福永賀予,河野崇,横山正尚,他
  • Organizer: 日本麻酔科学会第58回学術集会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2011-05-19
• [Presentation] 術後痛に対してNK1受容体拮抗薬アプレピタントは有効か? (2011)
  • Author(s): 廣橋美穂, 河野崇、横山正尚, 他
  • Organizer: 日本麻酔科学会第58回学術集会
  • Place of Presentation: 神戸,国際会議場
  • Year and Date: 2011-05-19
• [Presentation] von Frey filament刺激による逃避行動は神経障害モデル動物で侵害刺激反応であるか? (2011)
  • Author(s): 福永賀予, 河野崇, 横山正尚, 他
  • Organizer: 日本麻酔科学会第58回学術集会
  • Place of Presentation: 神戸,国際会議場
  • Year and Date: 2011-05-19
• [Presentation] 動物を用いた痛みの研究の基礎的アプローチ"Bench to Bedside" (2011)
  • Author(s): 河野崇
  • Organizer: 第21回日本ペインクリニック学会中国・四国合同地方会
  • Place of Presentation: 高知,総合あんしんセンター
  • Year and Date: 2011-05-14
• [Presentation] Comparison of oxygenation during one lung ventilation in prone position with lateral decubitus position in esophagectomy (2010)
  • Author(s): T.Yatabe, M.Hirohashi, M Yokoyama, et al
  • Organizer: Euroanaesthesia 2010
  • Place of Presentation: ヘルシンキコンベンションセンター
  • Year and Date: 2010-06-12
• [Presentation] The evaluation of the ability of the STG-22 to decrease the variability of blood glucose concentration in ICU patients. (2010)
  • Author(s): T.Yatabe, R.Yamazaki, M Yokoyama, et al
  • Organizer: Euroanaesthesia 2010
  • Place of Presentation: ヘルシンキコンベンションセンター
  • Year and Date: 2010-06-12
• [Presentation] 硬膜外穿刺は全身麻酔下に施行してもよいか (2010)
  • Author(s): 横山正尚
  • Organizer: 日本麻酔科学会第57回学術集会議
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2010-06-03
• [Presentation] 直腸癌の仙骨転移による疼痛の緩和に脊髄刺激療法が有効であった1症例 (2010)
  • Author(s): 神元裕子,穴山玲子,横山正尚
  • Organizer: 第20回日本ペインクリニック学会中国・四国合同地方会
  • Place of Presentation: 広島大学キャンパス広仁会館(広島市)
  • Year and Date: 2010-05-15
• [Presentation] 直腸癌の仙骨転移による疼痛の緩和に脊髄刺激療法が有効であった1症例 (2010)
  • Author(s): 神元裕子, 穴山玲子, 横山正尚
  • Organizer: 第20回日本ペインクリニック学会中国・四国合同地方会
  • Place of Presentation: 広島大学キャンパス広仁会館
  • Year and Date: 2010-05-15
• [Presentation] 術後鎮痛の上手な選び方、使い方「IV-PCAによる術後鎮痛」 (2009)
  • Author(s): 横山正尚
  • Organizer: 第29回日本臨床麻酔学会
  • Place of Presentation: 浜松コンベンションセンター(浜松市)
  • Year and Date: 2009-10-29
• [Presentation] Anti-Nociceptive Effect of Decoy ODN Inhibiting BDNF in Rat Inflammatory Pain Model (2009)
  • Author(s): Obata N, Yokoyama M, et al
  • Organizer: A merican Society of Anesthesiologists2009
  • Place of Presentation: New Orleans(USA)
  • Year and Date: 2009-10-17
• [Presentation] Anti-Nociceptive Effect of Decoy ODN Inhibiting BDNF in Rat Inflammatory (2009)
  • Author(s): 小幡典彦, 横山正尚, et al
  • Organizer: American Society of Anesthesiologists
  • Place of Presentation: New Orleans(USA)
  • Year and Date: 2009-10-17
• [Presentation] Rho-Kinase Inhibitors Augment Anesthetics-Induced Relaxation in Rat Airway Smooth Muscle (2009)
  • Author(s): Hanazaki M, Yokoyama M, et al
  • Organizer: American Society of Anesthesiologists
  • Place of Presentation: New Orleans(USA)
  • Year and Date: 2009-10-17
• [Presentation] 侵襲とストレス・疼痛反応.臨床研究と基礎研究より. (2009)
  • Author(s): 横山正尚
  • Organizer: 第46回日本麻酔科学会中国四国地方会
  • Place of Presentation: 川崎医科大学(倉敷市)
  • Year and Date: 2009-09-26
• [Presentation] 侵襲とストレス・疼痛反応-臨床研究と基礎研究より- (2009)
  • Author(s): 横山正尚
  • Organizer: 第46回日本麻酔科学会中国四国地方会
  • Place of Presentation: 川崎医科大学(倉敷市)
  • Year and Date: 2009-09-26
• [Presentation] 将来を見据えた麻酔科学 (2009)
  • Author(s): 横山正尚
  • Organizer: 第56回日本麻酔科学会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2009-08-16
• [Presentation] ラット炎症性疼痛モデルにおけるBDNFノックダウンデコイの疼痛抑制効果 (2009)
  • Author(s): 小幡典彦、横山正尚,他
  • Organizer: 第31回日本疼痛学会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2009-07-17
• [Presentation] 脳脊髄液減少症患者における脳脊髄液中シスタチンC濃度 (2009)
  • Author(s): 小幡典彦, 横山正尚, 他
  • Organizer: 第31回日本疼痛学会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2009-07-17
• [Presentation] Multicenter prospective study for hemodynamic changes during anesthetic induction : Remifentanil vs fentanyl (2009)
  • Author(s): Morimatsu H, Yokoyama M, et al
  • Organizer: Euroanaesthesia
  • Place of Presentation: Milan(Italy)
  • Year and Date: 2009-06-06
• [Book] 周術期管理チームテキスト2010:血液疾患患者の術前評価 (2010)
  • Author(s): 山下幸一, 横山正尚
  • Total Pages: 536
  • Publisher: 日本麻酔科学会
• [Book] 周術期管理チームテキスト2010:膠原病患者の術前評価 (2010)
  • Author(s): 矢田部智昭, 横山正尚
  • Total Pages: 536
  • Publisher: 日本麻酔科学会
• [Remarks]
  • URL: http://www.kochi-ms.ac.jp/~fm_ansth/index.htm