Exploration of a new molecular target of hormone refractory prostate cancer based on nuclear matrix proteins

• Project/Area Number: 21592043
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: INOUE Takahiro 京都大学, 医学研究科, 助教 (80511881)
• Co-Investigator(Kenkyū-buntansha): OGAWA Osamu 京都大学, 医学研究科, 教授 (90260611) ; 兼松 明弘 京都大学, 医学研究科, 講師 (90437202) ; 渡部 淳 京都大学, 医学研究科, 助教 (10452335)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: PURA LASP-1 / 前立腺癌 / 去勢抵抗性 / PURA / LASP-1 / LASP1 / ATF3 / GADD45A / S100P / HERPUD1

Research Abstract

The aim of this research is to analyze whether a nuclear matrix protein, Purine-element binding protein alpha(PURA), is useful for exploration for a new molecular target of hormone refractory prostate cancer.
Overexpression of PURA obviously lowered the growth speed of PC3 cells in vivo, and by immunohistochemistry, Ki67 expression was weaker in PC3 cells with PURA overexpression than in control PC3 cells. And we have established tissue microarray, including 22 cases of castration resistant prostate cancer, which were obtained by TUR. Using this microarray, we are planning to ask how those expression changes with progression of prostate cancer.

Research Products

• [Journal Article] Roleof Signaling Transduction Pathways inDevelopment of Castration-ResistantProstate Cancer (2011)
  • Author(s): Takahiro Inoue, Osamu Ogawa
  • Journal Title: Prostate Cancer Volume: 7 Pages: 647987-647987
  • DOI: 10.1155/2011/647987
  • Peer Reviewed
• [Journal Article] Long-term outcomes of three-dimensional conformal radiation therapy combined with neoadjuvant hormonal therapy for Japanese patients with T1C-T2NOMO prostate cancer (2011)
  • Author(s): Mizowaki T, Takayama K, Norihisa Y, Ogura M, Kamba T, Inoue T, Shimizu Y, Kamoto T, Ogawa O, Hiraoka M
  • Journal Title: Int J Clin Oncol. Volume: (印刷中) Issue: 6 Pages: 562-568
  • DOI: 10.1007/s10147-011-0326-z
  • Peer Reviewed
• [Journal Article] A potent chemotherapeutic strategy inprostate cancer : S-(methoxytrityl)-l-cysteine, a novel Eg5 inhibitor (2011)
  • Author(s): Xing ND, Ding ST, Saito R, Nishizawa K, Kobayashi T, Inoue T, Oishi S, Fujii N, Lv JJ, Ogawa O, Nishiyama H
  • Journal Title: Asian J Androl Volume: 13 Issue: 2 Pages: 236-41
  • DOI: 10.1038/aja.2010.171
• [Journal Article] Spa-1 (Sipa1) controlsthe invasion and metastasis of human prostatecancers via Rap signal (2011)
  • Author(s): Shimizu Y, Hamazaki Y, Hattori M, Doi K,Terada Y, Kobayashi T, Toda Y, Yamasak T,Inoue T, Kajita Y, Atsushi M, Kamba T, MikamiY, Kamoto T, Yamada T, Ogawa O, Minato Nand Nakamura E
  • Journal Title: Cancer Science Volume: 102 Issue: 4 Pages: 828-836
  • DOI: 10.1111/j.1349-7006.2011.01876.x
  • Peer Reviewed
• [Journal Article] アンドロゲン非依存性進展に伴うシグナル伝達異常 (2011)
  • Author(s): 井上貴博
  • Journal Title: 日本臨床増刊号前立腺癌(第二版) Volume: 69,Suppl5 Pages: 121-125
• [Journal Article] Activationof Rac1 Is Closely Related toAndrogen-Independent Cell Proliferationof Prostate Cancer Cells Both in Vitro andin Vivo (2010)
  • Author(s): Kobayashi T, Inoue T, Shimizu Y, Terada N, Maeno A, Kajita Y, Yamasaki T, Kamba T, Toda Y, Mikami Y, Yamada T, Kamoto T, Ogawa O, Nakamura E
  • Journal Title: Mol Endocrinol Volume: 24 Issue: 4 Pages: 722-34
  • DOI: 10.1210/me.2009-0326
• [Journal Article] Identification of EP4as a Potential Target for the Treatment ofCastration-Resistant Prostate CancerUsing a Novel Xenograft Model (2010)
  • Author(s): Terada N, Shimizu Y, Kamba T, Inoue T, Maeno A, Kobayashi T, Nakamura E, Kamoto T, Kanaji T, Maruyama T, Mikami Y, Toda Y, Matsuoka T, Okuno Y, Tsujimoto G, Narumiya S, Ogawa O
  • Journal Title: Cancer Res Volume: 70 Issue: 4 Pages: 1606-15
  • DOI: 10.1158/0008-5472.can-09-2984
• [Journal Article] Regulation ofandrogen receptor transactivity andmTOR-S6 kinase pathway by Rheb in prostatecancer cell proliferation (2010)
  • Author(s): Takashi Kobayashi, Yosuke Shimizu, Naoki Terada, Toshinari Yamasaki, Eijiro Nakamura, Yoshinobu Toda, Hiroyuki Nishiyama, Toshiyuki Kamoto, Osamu Ogawa and, Takahiro Inoue
  • Journal Title: Prostate Volume: 70 Issue: 8 Pages: 866-874
  • DOI: 10.1002/pros.21120
• [Journal Article] Osamu OgawaAntiandrogen Withdrawal Syndrome andAlternative Antiandrogen TherapyAssociated with the W741C Mutant AndrogenReceptor in a Novel Prostate CancerXenograft (2009)
  • Author(s): Naoki Terada, Yosuke Shimizu, Toru Yoshida, Atsushi Maeno, Tomomi Kamba, Takahiro Inoue, Eijiro Nakamura, Toshiyuki Kamoto
  • Journal Title: Prostate Volume: 70 Issue: 3 Pages: 252-261
  • DOI: 10.1002/pros.21058
• [Journal Article] Restorationof cyclin D2 has an inhibitory potentialon the proliferation of LNCaP cells (2009)
  • Author(s): Kobayashi T, Nakamura E, Shimizu Y, Terada N, Maeno A, Kobori G, Kamba T, Kamoto T, Ogawa O, Inoue T
  • Journal Title: Biochem Biophys Res Commun Volume: 387(1) Issue: 1 Pages: 196-201
  • DOI: 10.1016/j.bbrc.2009.06.146
• [Journal Article] Down-regulating cold shockprotein genes impairs cancer cell survivaland enhances chemosensitivity (2009)
  • Author(s): Zeng Y, Kulkarni P, Inoue T, Getzenberg RH.
  • Journal Title: J CellBiochem Volume: 107 Issue: 1 Pages: 179-88
  • DOI: 10.1002/jcb.22114
• [Journal Article] Purine-rich elementbinding protein(PUR) alpha inducesendoplasmic reticulum stress response, and cell differentiation pathways inprostate cancer cells (2009)
  • Author(s): Inoue T, Maeno A, Talbot C Jr, Zeng Y, Yeater DB, Leman ES, Kulkarni P, Ogawa O, Getzenberg RH.
  • Journal Title: Prostate Volume: 69 Issue: 8 Pages: 861-73
  • DOI: 10.1002/pros.20936
• [Journal Article] Purine-rich element binding protein (PUR) alpha induces endoplasmic reticulum stress response, and cell differentiation pathways in prostate cancer cells. (2009)
  • Author(s): Inoue T.
  • Journal Title: Prostate 69 Pages: 861-873
  • Peer Reviewed
• [Presentation] 活性型FAK (Focal adhesion kinase)の発現は前立腺がん全摘後の生化学的再発と相関する (2011)
  • Author(s): 井上貴博、小林恭、前野淳、赤松秀輔、岡田能幸、吉川武志、清水洋祐、神波大己、小川修
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] 日本人における前立腺癌のゲノムワイド関連解析のフォローアップスタディーにより新たに4つの前立腺癌罹患関連領域が同定された (2011)
  • Author(s): 赤松秀輔、高田亮、井上貴博、藤岡知昭、穎川晋、羽渕友則、小川修、中村祐輔、中川英刀
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] 嚢胞状腫瘍を形成する新規前立腺癌異種移植片モデル (2011)
  • Author(s): 小堀豪、井上貴博、岡田能幸、前野淳、寺田直樹、清水洋祐、神波大己、小川修
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-04-24
• [Presentation] 去勢抵抗性前立腺癌の治療標的探索のための新規xenograftにおけるmicroRNA発現解析 (2011)
  • Author(s): 前野淳、岡田能幸、小堀豪、赤松秀輔、清水洋祐、井上貴博、神波大己、小川修
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-04-23
• [Presentation] 前立腺癌根治的治療後のPSA再発に対するbicalutamide単剤間歇療法の検討 (2011)
  • Author(s): 井上貴博、神波大己、清水洋祐、松井喜之、西山博之、賀本敏行、小川修
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-04-21
• [Presentation] 核マトリックス蛋白質Purine-rich element binding protein alpha (PURA)はアンドロゲン非依存性前立腺癌細胞の増殖を抑制する (2009)
  • Author(s): 前野淳
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-02
• [Remarks]
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/