The research on the usefulness of stem cells derrived adipose tissue for reconering renal funcion after partail nephrectomy

• Project/Area Number: 21592071
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Nagoya University
• Principal Investigator: SASSA Naoto 名古屋大学, 医学部附属病院, 助教 (50437026)
• Co-Investigator(Kenkyū-buntansha): HATTORI Ryohei 名古屋大学, 医学系研究科, 准教授 (20324410) ; YAMAMOTO Tokunori 名古屋大学, 医学部附属病院, 講師 (20182636) ; 舟橋 康人 名古屋大学, 医学部附属病院, 病院助教 (70534824)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
• Keywords: 脂肪由来幹細胞 / 腎部分切除術 / 尿細管周囲毛細血管血流 / 時空間画像処理 / 腎部分切除後の腎機能回復 / 尿路管周囲毛細血管血流 / 脂肪脂肪由来幹細胞 / 腹腔鏡手術 / 気腹圧 / 腎微小循環

Research Abstract

Background.
Acute renal ischemic injury(AKI) represents a major clinical problem with renal arterial clamp at parial nephrectony. The use of therapy using adipose tissue-derived stem cells(ASCs) has been suggested as a potential modality to attenutate the ischemic renal damage. Methods. We investigated the possible renoprotection of pretreatment of ASCs before and after in a rat ischemia. reperfusion(I. R) model of AKI. Twenty-four hours postischemia, blood flow in peritubular capillaries(PTC) was measured using intravital videomicroscopy.
Results.
We demonstrated that ADRC therapy significantly reduced serum creatinine and BUN. Histological analysis further validated a significantly attenuated tubular damage. Intravital videomicroscopy and measurement of red blood cell velocity in peritubular capillaries showed ASCs-injected kidneys displayed significant hemodynamic improvement.
Conclusions.
The subscapular administration of ASCs to the kidney attenuates I/R renal injury though antiinflammation, antiapotoyic effect and peritubular capillary microcirculation. The present study suggests that ASCs would be a useful tool in preventing ishemic kidney damage in the clinical setting.

Research Products

• [Journal Article] Low serum cultured adipose tissue-derived stromal cells ameliorate acute kidney injury in rats (2012)
  • Author(s): Katsuno T, Ozaki T, Furuhashi K, Kim H, Yasuda K, Yamamoto T, Sato W, Tsuboi N, Mizuno M, Ito Y, Imai E, Matsuo S, Matuyama S
  • Journal Title: Cell Transplantation
• [Journal Article] Periurethral injection of autologous adipose-derived regenerative cells for the treatment of male stress uri nary incontinence (2012)
  • Author(s): Yamamoto T, Gotoh M, Kato M, Majima T, Toriyama T, Kamei Y, Iwaguro H, Mastukawa Y, Funahashi Y
  • Journal Title: J Urol
• [Journal Article] Effect of warm ischemia on renal function during partial nephrectomy : assessment with new 99mTc-mercaptoacet yltriglycine scintigra phyparameter (2012)
  • Author(s): Funahashi Y, Hattori R, Yamamoto T, Sassa N, Fujita T, Gotoh M
  • Journal Title: Urology Volume: 79(1) Pages: 160-4
• [Journal Article] Subclassification of pT3 Urothelial Carcinoma of the Renal Pelvicalyceal System is Associated With Recurrence-Free and Cancer-Specific Survival : Proposal for a Revision of the Current TNM Classification (2012)
  • Author(s): Tsuzuki T, Sassa N, Gotoh M Re : Yann Neuzillet, Xavier Tillou, Re : Shahrokh F. Shariat, Richard Zigeuner, Michael Rink
  • Journal Title: Eur Urol Volume: (In press) Pages: 23-23
• [Journal Article] Periurethral injection of autologous adipose-derived regenerative cells for the treatment of male stress urinary incontinence : report of 3 initial cases (2012)
  • Author(s): Yamamoto T, Gotoh M, Kato M, Majima T, Toriyama T, Kamei Y, Iwaguro H, Mastukawa Y, Funahashi Y
  • Journal Title: Int J Urol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Renal cell carcinoma(RCC) in patients with end-stage renal disease ex hibits many favourable clinical, patholo gic, and outcome features compared with RCC in the general population (2011)
  • Author(s): Tsuzuki T, Sassa N, Gotoh M. Re : Yann N euzillet, Xavier Tillou, Romain Mathieu, et al.
  • Journal Title: Eur Uro Volume: 60(4) Pages: 35-36
• [Journal Article] The post-operative pathological prognostic parameters of clear cell renal cell carcinoma in pT1a cases (2011)
  • Author(s): Nishikimi T, Tsuzuki T, Fujita T, Sassa N, Fukatsu A, Katsuno S, Yoshino Y, Hattori R, Gotoh M
  • Journal Title: Pathol Int Volume: 61(3) Pages: 116-21
  • NAID: 10031185059
• [Journal Article] Renal cell carcinomas in haemodialysis patients : does haemodialysis duration influence pathological cell types and prognosis (2011)
  • Author(s): Sassa N, Hattori R, Tsuzuki T, Watarai Y, Fukatsu A, Katsuno S, Nishikimi T, Fujita T, Ohmae K, Gotoh M
  • Journal Title: Nephrol Dial Transplant Volume: 26(5) Pages: 1677-82
• [Journal Article] Re : Yann Neuzillet, Xavier Tillou, Romain Mathieu, et al.Renal cell carcinoma (RCC) in patients with end-stage renal disease exhibits many favourable clinical, pathologic, and outcome features compared with RCC in the general population (2011)
  • Author(s): Tsuzuki T, Sassa N, Gotoh M.
  • Journal Title: Eur Urol. Volume: 60(4)
  • Peer Reviewed
• [Journal Article] The post-operative pathological prognostic parameters of clear cell renal cell carcinoma in pT1a cases (2011)
  • Author(s): Nishikimi T, Tsuzuki T, Fujita T, Sassa N, Fukatsu A, Katsuno S, Yoshino Y, Hattori R, Gotoh M
  • Journal Title: Pathol Int. Volume: 61(3) Pages: 116-21
  • NAID: 10031185059
  • Peer Reviewed
• [Journal Article] Relationship between renal parenchymal volume and single kidney glomerular filtration rate before and after unilateral nephrectomy (2011)
  • Author(s): Funahashi Y, Hattori R, Yamamoto T, Kamihira O, Sassa N, Gotoh M
  • Journal Title: Urology Volume: 77(6) Pages: 1404-8
  • Peer Reviewed
• [Journal Article] Renal cell carcinomas in haemodialysis patients : does haemodialysis duration influence pathological cell types and prognosis? (2011)
  • Author(s): Sassa N, Hattori R, Tsuzuki T, Watarai Y, Fukatsu A, Katsuno S, Nishikimi T, Fujita T, Ohmae K, Gotoh M
  • Journal Title: Nephrol Dial Transplant Volume: 26(5) Pages: 1677-82
  • Peer Reviewed
• [Journal Article] Clinical efficacy of a loading dose of naftopidil for patients with benign prostate hyperplasia (2011)
  • Author(s): Funahashi Y, Hattori R, Matsukawa Y, Komatsu T, Sassa N, Gotoh M
  • Journal Title: World J Urol. Volume: 29(2) Pages: 225-3
  • Peer Reviewed
• [Journal Article] De novo detrusor under activity after laparoscopic radical prostatectomy (2010)
  • Author(s): Matsukawa Y, Hattori R, Komatsu T, Funahashi Y, Sassa N, Gotoh M
  • Journal Title: Int J Urol Volume: 17(7) Pages: 643-8
• [Journal Article] Effect of warm ischemia on renal function during partial nephrectomy : asse ssment with new 99mTc-mercaptoace tyltriglycine scintigraphy parameter (2010)
  • Author(s): Funahashi Y, Hattori R, Yamamoto T, Sassa N, Fujita T
  • Journal Title: Urology Volume: 79(1) Pages: 60-64
• [Journal Article] Renal cell carcinomas in haemodialysis patients : does haemodialysis duration influence pathological cell types and prognosis? (2010)
  • Author(s): N.Sasa, R.Hattori, T.Tsuzuki, Y.Watarai, A.Fukatsu, S.Katsuno, T.Nishikimi, T.Fujita, K.Ohmae, M.Gotoh.
  • Journal Title: Nephrol Dial Transplant Volume: 73(2) Pages: 1677-1682
  • Peer Reviewed
• [Journal Article] Direct visualization of renal hemodynamics affected by carbon dioxide-induced pneumoperitoneum (2009)
  • Author(s): Sassa N, Hattori R, Yamamoto T, Kato M, Komatsu T, Matsukawa Y, Funahashi Y, Gotoh M
  • Journal Title: Urology Volume: 73(2) Pages: 311-5
• [Journal Article] Direct visualization of renal hemodynamics affected by carbon-dioxide in duced pneumoperitoneum (2009)
  • Author(s): N.Sassa, R.Hattori, T.Yamamoto, M.Kato, T.Komatsu, Y.Matsukawa, Y.Funahashi, M.Gotoh
  • Journal Title: Urology 73(2) Pages: 311-315
  • Peer Reviewed
• [Presentation] 名古屋大学関連施設における尿管腫瘍の予後因子の検討 (2011)
  • Author(s): 佐々直人
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋
• [Presentation] 名古屋大学におけるmRCCに対する分子標的剤治療成績 (2011)
  • Author(s): 佐々直人
  • Organizer: 第49回日本癌治療学会
  • Place of Presentation: 名古屋
• [Presentation] 尿路上皮癌に対する11C-choline PET-CTの有用性の検討 (2010)
  • Author(s): 佐々直人、山本徳則、服部良平、後藤百万、加藤克彦
  • Organizer: 第48回日本癌治療学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-30
• [Presentation] 全国のクリニカルパス研究における、経尿道的前立腺手術の患者満足度調査 (2010)
  • Author(s): 佐々直人、荒井陽一、川喜田睦司、津島知靖、内藤誠二、野尻佳克、長谷川友紀、服部良平、松田公志、岡村菊夫
  • Organizer: 第24回日本Endourology・ESWL学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-23
• [Presentation] 腎孟癌におけるstagingを中心とした病理組織学的再検討～pT3は本当に臨床予後を反映しているか?～ (2010)
  • Author(s): 佐々直人、都築豊徳、小林弘明、深津顕俊、木村亨、馬嶋剛、服部良平、後藤百万
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-04-28
• [Presentation] 腎盂癌におけるstagingを中心とした病理組織学的再検討 (2010)
  • Author(s): 佐々直人
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 岩手
• [Presentation] 尿路上皮癌に対する111C-choline-PETの有用性の検討 (2010)
  • Author(s): 佐々直人
  • Organizer: 第48回日本癌治療学会
  • Place of Presentation: 京都
• [Presentation] 全国のクリニカルパス研究における経尿道的前立腺手術の患者満足度調査 (2010)
  • Author(s): 佐々直人
  • Organizer: 第24回日本Endurology/ESWL学会総会
  • Place of Presentation: 京都
• [Presentation] 透析腎癌の臨床病理学的検討 (2009)
  • Author(s): 佐々直人、服部良平、吉野能、都築豊徳、後藤百万
  • Organizer: 第97回日本泌尿器科学会総会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-04-17
• [Presentation] 透析腎癌の臨床病理学的検討 (2009)
  • Author(s): 佐々直人
  • Organizer: 第97回日本泌尿器科学会総会
  • Place of Presentation: 岡山
• [Presentation] 名古屋大学におけるGemcitabine化学療法の検討 (2009)
  • Author(s): 佐々直人
  • Organizer: 第47回日本癌治療学会
  • Place of Presentation: 横浜