• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Uroplakin III-delta 4 as a new molecular marker for interstitial cystitis

Research Project

Project/Area Number 21592074
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKagawa University

Principal Investigator

KAKEHI Yoshiyuki (2010-2011)  香川大学, 医学部, 教授 (20214273)

植月 祐次 (2009)  Kagawa University, 医学部, 助教 (50524063)

Co-Investigator(Kenkyū-buntansha) TYOU Ka  香川大学, 医学部, 助教 (30524061)
WU Xiu-xian  香川大学, 医学部, 助教 (10346645)
INUI Masashi  香川大学, 医学部附属病院, 講師 (40314918)
SUGIMOTO Mikio  香川大学, 医学部附属病院, 准教授 (10243768)
HONMA Yukio  東京大学, 医学部, 教授 (40165626)
YOSHIKI Tatsuhiro  京都薬科大学, 薬学部, 教授 (80230704)
常森 寛行  香川大学, 医学部附属病院, 助教 (20380173)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords間質性膀胱炎 / ウロプラキン / 非潰瘍型 / ウロプラキンデルタ4 / 分子診断 / ウロプラキンIIIデルタ4
Research Abstract

Interstitial cystitis(IC) is a chronic bladder disorder affecting approximately one million people in the United States, of whom some 90% are women. IC is characterized by urinary frequency, urinary urgency, bladder discomfort or bladder pain in the absence of any identifiable cause, such as bacterial infection. Due to lacking in a reliably objective diagnostic test, IC remains a diagnosis based on symptoms and exclusion criteria.
The apical surface of mammalian urothelium is covered by numerous rigid-appearing plaques that contribute to the permeability barrier. Uroplakins(UPs) Ia, Ib, II, and III consist of these plaques by forming heterodimer pairs(UPIa/ UPII and UPIb/ UPIII). UPIII-delta 4(UP3d4) is a splicing variant of UPIII that can be distinguished from UPIII by lacking exon 4. The whole cDNA fragment for human UP3d4 was molecularly cloned by us from a cDNA library constructed from bladder mucosa samples of a patient with vesicoureteral reflux. We investigated gene expression pr … More ofile of UPs including UP3d4 in bladder mucosa of patients with IC. One of the major findings of the study was that UP3d4 gene was significantly up-regulated in IC samples. What was more striking was that up-regulation of UP3d4 was specifically observed in non-ulcerative type IC bladder samples.
The next step of this study project is to investigate protein expression of UP3d4 in bladder urothelial cells of IC patients. For this purpose, we have raised murine monoclonal antibodies against purified UP3d4 protein which was produced by the recombinant DNA technique. Protein expression of UP3d4 was evaluable in 46 IC patients while 27 were not due to epithelial exfoliation. UP3d4 was immunohistochemically positive in 29 of 46 samples(63%). The positive rate was 80%(20/ 25) in non-ulcer type and 43%(9/ 21) in ulcer type IC(p=0. 014). UP3d4 was not detected in any of non-IC bladder mucosa. Patients with positive UP3d4 staining was younger than those with negative staining(mean age : 51. 5 vs 62. 0, p=0. 029). UP3d4 mRNA expression was identified in urine sediment cells from 24%(8/ 33) IC patients ; 18%(3/ 17) in non-ulcer type and 31%(5/ 16) ulcer type.
In conclusion, a high UP3d4 protein expression was demonstrated in urothelium of IC, especially non-ulcer type IC. Detection of UP3d4 mRNA was feasible for urine sediment cells. Further exploration is warranted to investigate the potential role of UP3d4 in pathogenesis and diagnosis of IC. Less

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (9 results)

All 2011 2010 2009 2007 Other

All Journal Article (3 results) Presentation (4 results) Remarks (2 results)

  • [Journal Article] 間質性膀胱炎とウロプラキン2010

    • Author(s)
      張霞
    • Journal Title

      Urology View Vol.8No.1

      Pages: 19-22

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Uroplakin III-delta4 messenger RNA as a promising marker to identify nonulcerative interstitial cystitis2007

    • Author(s)
      Zeng Y, Kakehi Y, et al.
    • Journal Title

      J Urol

      Volume: 178 Pages: 1322-7

    • Related Report
      2011 Final Research Report
  • [Journal Article] 特集間質性膀胱炎の最前線;病態の解明(3)-分子マーカー・遺伝子マーカー2007

    • Author(s)
      筧善行
    • Journal Title

      排尿障害プラクティス

      Volume: 15 Pages: 233-237

    • Related Report
      2011 Final Research Report
  • [Presentation] 間質性膀胱炎患者膀胱上皮および尿中剥離細胞におけるウロプラキンIII-delta4の発現2011

    • Author(s)
      筧善行, 張霞, 平間裕美, 加藤琢磨, 本間之夫, 大橋洋三, 影山進, 吉貴達寛
    • Organizer
      第18回日本排尿機能学会
    • Place of Presentation
      福井市
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ウロプラキンと間質性膀胱炎2010

    • Author(s)
      筧善行
    • Organizer
      第12回UTPシンポジウム
    • Place of Presentation
      京都市
    • Year and Date
      2010-01-16
    • Related Report
      2011 Final Research Report
  • [Presentation] ウロプラキンと間質性膀胱炎2010

    • Author(s)
      筧善行
    • Organizer
      第12回UTPシンポジウム
    • Place of Presentation
      東京都(フォーシーズンズホテル)
    • Year and Date
      2010-01-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] 間質性膀胱炎-潰瘍型と非潰瘍型での治療戦略の違い-2009

    • Author(s)
      武田繁雄, 平間裕美, 筧善行
    • Place of Presentation
      岡山市
    • Year and Date
      2009-02-20
    • Related Report
      2011 Final Research Report
  • [Remarks] 本研究内容が科学技術振興機構A-STEP(研究成果最適展開支援事業)に採択される

    • Related Report
      2011 Annual Research Report
  • [Remarks] 本研究内容が科学技術振興機構 A-STEP(研究成果最適展開支援事業)に採択される

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi