| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
In vitro reconstitution of spermatogenesis is a very difficult task and remained unsuccessful for a long time. We challenged that task using classical organ culture method, called air-liquid interphase. Through testing variety of culture conditions, we found that a serum replacement, KSR, is effective and induced complete spermatogenesis of mouse. Offspring were obtained with the sperm produced in vitro. We also showed that cryopreservation of testis tissue fragment is possible to preserve spermatogenic ability of the tissues.
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