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Functional analyses of mammalian round spermatid for fertilization

Research Project

Project/Area Number 21592086
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTohoku University

Principal Investigator

HAYASAKA Shinichi  東北大学, 大学院・医学系研究科, 非常勤講師 (00535099)

Co-Investigator(Kenkyū-buntansha) TERADA Yukihiro  秋田大学, 大学院・医学系研究科, 教授 (10260431)
TAKANO Tadao  東北大学, 未来医工学治療開発センター, 准教授 (40282058)
OTSUKI Takeo  東北大学, 病院, 助教 (40531330)
NABESHIMA Hiroshi  東北大学, 病院, 助教 (90547415)
HASEGAWA Hisataka  東北大学, 病院, 技能補佐員 (20455835)
IMAI Noriaki  東北大学, 大学院・医学系研究科, 非常勤講師 (60531332)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords円形精子細胞 / 精子中心体 / 補助活性化法 / 受精障害 / 生殖補助技術 / 受精能 / 発生能 / 不妊症
Research Abstract

The goal of the fertilization assumes he fusion of the sex genome in the egg cytoplasm. We examined fertilization establishment ability of the round spermatid of the mammal that was assumed to be extremely low, using human and a laboratory animal (a mouse, rabbit). Sperm function to accomplish a major event of the fertilization after the sperm entry, oocyte activation ability and sperm centrosomal function were found to be decreased. Furthermore, as one of the methods to supplement ability for oocyte activation that decreased with round spermatid, we developed the co-culture system with hormone addition and Sertoli cells of the mouse round spermatid.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (25 results)

All 2011 2010 2009

All Journal Article (23 results) (of which Peer Reviewed: 23 results) Presentation (2 results)

  • [Journal Article] Risk factors for recurrence and re-recurrence of ovarian endometriomas after laparoscopic excision2011

    • Author(s)
      Hayasaka S, Ugajin T, Fujii O, Nabeshima H, Utsunomiya H, Yokomizo R, Yuki H, Terada Y, Murakami T, Yaegashi N
    • Journal Title

      J Obstet Gynaecol Res

      Volume: 37(6) Pages: 581-585

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Risk factors for recurrence and re-recurrence of ovarian endometriomas after laparoscopic excision2011

    • Author(s)
      Hayasaka, S., Ugajin, T., Fujii, O., Nabeshima, H., Utsunomiya, H., Yokomizo, R., Yuki, H., Terada, Y., Murakami, T., Yaegashi, N.
    • Journal Title

      J Obstet Gynaecol Res

      Volume: 37 Pages: 581-5

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Total laparoscopic conservative surgery for an intramural ectopic pregnancy.2011

    • Author(s)
      Nabeshima H, Nishimoto M, Utsunomiya H, Arai M, Ugajin T, Terada Y, Yaegashi N.
    • Journal Title

      Diagn Ther Endosc.

      Volume: 未定(印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Risk factors for recurrence and re-recurrence of ovarian endometriomas after laparoscopic excision.2011

    • Author(s)
      Hayasaka S, Ugajin T, Fujii O, Nabesbima H, Utsunomiya H, Yokomizo R, Yuki H, Terada Y, Murakami T, Yaegashi N.
    • Journal Title

      J Obstet Gynaecol Res.

      Volume: 未定(印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Total laparoscopic conservative surgery for an intramural ectopic pregnancy2010

    • Author(s)
      Nabeshima H, Nishimoto M, Utsunomiya H, Arai M, Ugajin T, Terada Y, Yaegashi N
    • Journal Title

      Diagn Ther Endosc

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Laparoscopic management of recurrent rupture of an adnexal mass in the second trimester of pregnancy2010

    • Author(s)
      Hayasaka S, Murakami T, Nabeshima H, Terada Y, Yaegashi N
    • Journal Title

      A case report Asian Journal of Endoscopic Surgery

      Volume: 3 Pages: 201-203

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function2010

    • Author(s)
      UgajinT, Terada Y, Hasegawa H, Nabeshima H, Suzuki K, Yaegashi N
    • Journal Title

      Assist Reprod Genetics

      Volume: 27(2-3) Pages: 75-81

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant behavior of mouse embryo development after blastomere biopsy as observed through time-lapse cinematography2010

    • Author(s)
      UgajinT, Terada Y, Hasegawa H, Velayo CL, Nabeshima H, Yaegashi N
    • Journal Title

      Fertil Steril

      Volume: 93 Pages: 2723-2728

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Laparoscopic removal of the rudimental uterus followed by colpopoiesis in a Mayer-Rokitansky-Kuster-Hauser syndrome patient with a functional endometrium and peritoneal endometriosis2010

    • Author(s)
      Terada Y, Ugajin T, Arai-Kikuchi M, Hayasaka S, Nabeshima H, Yaegashi N
    • Journal Title

      J Gynecol Surg

      Volume: 26 Pages: 201-203

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Essential roles of the sperm centrosome in human fertilization : developing the therapy for fertilization failure due to sperm centrosomal dysfunction2010

    • Author(s)
      Terada Y, Schatten G, Hasegawa H, Yaegashi N
    • Journal Title

      Tohoku J Exp Med

      Volume: 220(4) Pages: 247-258

    • NAID

      10027083694

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of Long-Term Model throughout Regular Menstrual Cycles in Immunodeficient Mice2010

    • Author(s)
      Kikuchi-Arai M, Murakami T, Utsunomiya H, Akahira JI, Suzuki-Kakisaka H, Terada Y, Tachibana M, Hayasaka S, Ugajin T, Yaegashi N
    • Journal Title

      Am J Reprod Immunol

      Volume: 64(5) Pages: 324-332

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel culture system for mouse spermatid maturation which produces elongating spermatids capable of inducing calcium oscillation during fertilization and embryonic development2010

    • Author(s)
      Hasegawa H, Terada Y, Ugajin T, Yaegashi N, Sato K
    • Journal Title

      J Assist Reprod Genet

      Volume: 27(9-10) Pages: 565-570

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Laparoscopic management of recurrent rupture of an adnexal mass in the second trimester of pregnancy : A case report2010

    • Author(s)
      Hayasaka S, Murakami T, Nabeshima H, Terada Y, Yaegashi N
    • Journal Title

      Asian Journal of Endoscopic Surgery

      Volume: 3 Pages: 201-203

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function.2010

    • Author(s)
      Ugajin T, Terada Y, Hasegawa H, Nabeshima H, Suzuki K, Yaegashi N
    • Journal Title

      Assist Reprod Genetics

      Volume: 27(2-3) Pages: 75-81

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant behavior of mouse embryo development after blastomerebiopsy as observed through time-lapse cinematography.2010

    • Author(s)
      Ugajin T, Terada Y, Hasegawa H, Velayo CL, Nabeshima H, Yaegashi N
    • Journal Title

      Fertil Steril

      Volume: 93 Pages: 2723-2728

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Laparoscopic removal of the rudimental uterus followed by colpopoiesis in a Mayer-Rokitansky-Kuster-Hauser syndrome patiellt with a functional endometrium and peritoneal endometriosis.2010

    • Author(s)
      Terada Y, Ugajin T, Arai-Kikuchi M, Hayasaka S, Nabeshima H, Yaegashi N
    • Journal Title

      J Gynecol Surg

      Volume: 26 Pages: 201-203

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Essential roles of the sperm centrosome in human fertilization : developing the therapy for fertilization failure due to sperm centrosomal dysfunction.2010

    • Author(s)
      Terada Y, Schatten G, Hasegawa H, Yaegashi N
    • Journal Title

      Tohoku J Exp Med.

      Volume: 220(4) Pages: 247-258

    • NAID

      10027083694

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of Long-Term Model throughout Regular Menstrual Cycles in Immunodeficient Mice.2010

    • Author(s)
      Kikuchi-Arai M, Murakami T, Utsunomiya H, Akahira JI, Suzuki-Kakisaka H, Terada Y, Tachibana M, Hayasaka S, Ugajin T, Yaegashi N
    • Journal Title

      Am J Reprod Immunol.

      Volume: 64(5) Pages: 324-332

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel culture system for mouse spermatid maturation which produces elongating spermatids capable of inducing calcium oscillation during fertilization and embryonic development.2010

    • Author(s)
      Hasegawa H, Terada Y, Ugajin T, Yaegashi N, Sato K
    • Journal Title

      J Assist Reprod Genet.

      Volume: 27(9-10) Pages: 565-570

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Laparoscopic removal of the rudimental uterus followed by colpopoiesis in a Mayer-Rokitansky-Kuster-Hauser syndrome patient with a functional endometrium and peritoneal endometriosis.2010

    • Author(s)
      Terada Y, Ugajin T, Arai-Kikuchi M, Hayasaka S, Nabeshima H, Yaegashi N
    • Journal Title

      J Gynecol Surg (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Laparoscopic management of recurrent rupture of an adnexal mass in the second trimester of pregnancy : A case report2010

    • Author(s)
      Hayasaka S, Murakami T, Nabeshima H, Terada Y, Yaegashi N
    • Journal Title

      Asian Journal of Endoscopic (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Method for Safe Hysteroscopic Synechiolysis in Patients with Asherman Syndrome.2010

    • Author(s)
      Hayasaka S, Murakami T, Arai M, Ugajin T, Nabeshima H, Yuki H, Terada Y, Yaegashi N
    • Journal Title

      J Gyncol Surg (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Method for Safe Hysteroscopic Synechiolysis in Patients with Asherman Syndrome2009

    • Author(s)
      Hayasaka S, Murakami T, Arai M, Ugajin T, Nabeshima H, Yuki H, Terada Y, Yaegashi N
    • Journal Title

      J Gyncol Surg

      Volume: 25 Pages: 147-52

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] Post ICSI events in fertilizationに関わる配偶子の質について2010

    • Author(s)
      寺田幸弘
    • Organizer
      日本生殖医学会RMBシンポジウム
    • Place of Presentation
      徳島
    • Year and Date
      2010-11-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Post ICSI events in fertilizationに関わる配偶子の質について2010

    • Author(s)
      寺田幸弘
    • Organizer
      日本生殖医学会 RMBシンポジウム
    • Place of Presentation
      徳島
    • Year and Date
      2010-11-20
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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