Functional analyses of mammalian round spermatid for fertilization

• Project/Area Number: 21592086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: HAYASAKA Shinichi 東北大学, 大学院・医学系研究科, 非常勤講師 (00535099)
• Co-Investigator(Kenkyū-buntansha): TERADA Yukihiro 秋田大学, 大学院・医学系研究科, 教授 (10260431) ; TAKANO Tadao 東北大学, 未来医工学治療開発センター, 准教授 (40282058) ; OTSUKI Takeo 東北大学, 病院, 助教 (40531330) ; NABESHIMA Hiroshi 東北大学, 病院, 助教 (90547415) ; HASEGAWA Hisataka 東北大学, 病院, 技能補佐員 (20455835) ; IMAI Noriaki 東北大学, 大学院・医学系研究科, 非常勤講師 (60531332)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 円形精子細胞 / 精子中心体 / 補助活性化法 / 受精障害 / 生殖補助技術 / 受精能 / 発生能 / 不妊症

Research Abstract

The goal of the fertilization assumes he fusion of the sex genome in the egg cytoplasm. We examined fertilization establishment ability of the round spermatid of the mammal that was assumed to be extremely low, using human and a laboratory animal (a mouse, rabbit). Sperm function to accomplish a major event of the fertilization after the sperm entry, oocyte activation ability and sperm centrosomal function were found to be decreased. Furthermore, as one of the methods to supplement ability for oocyte activation that decreased with round spermatid, we developed the co-culture system with hormone addition and Sertoli cells of the mouse round spermatid.

Research Products

• [Journal Article] Risk factors for recurrence and re-recurrence of ovarian endometriomas after laparoscopic excision (2011)
  • Author(s): Hayasaka S, Ugajin T, Fujii O, Nabeshima H, Utsunomiya H, Yokomizo R, Yuki H, Terada Y, Murakami T, Yaegashi N
  • Journal Title: J Obstet Gynaecol Res Volume: 37(6) Pages: 581-585
  • Peer Reviewed
• [Journal Article] Risk factors for recurrence and re-recurrence of ovarian endometriomas after laparoscopic excision (2011)
  • Author(s): Hayasaka, S., Ugajin, T., Fujii, O., Nabeshima, H., Utsunomiya, H., Yokomizo, R., Yuki, H., Terada, Y., Murakami, T., Yaegashi, N.
  • Journal Title: J Obstet Gynaecol Res Volume: 37 Pages: 581-5
  • Peer Reviewed
• [Journal Article] Total laparoscopic conservative surgery for an intramural ectopic pregnancy. (2011)
  • Author(s): Nabeshima H, Nishimoto M, Utsunomiya H, Arai M, Ugajin T, Terada Y, Yaegashi N.
  • Journal Title: Diagn Ther Endosc. Volume: 未定(印刷中)
  • Peer Reviewed
• [Journal Article] Risk factors for recurrence and re-recurrence of ovarian endometriomas after laparoscopic excision. (2011)
  • Author(s): Hayasaka S, Ugajin T, Fujii O, Nabesbima H, Utsunomiya H, Yokomizo R, Yuki H, Terada Y, Murakami T, Yaegashi N.
  • Journal Title: J Obstet Gynaecol Res. Volume: 未定(印刷中)
  • Peer Reviewed
• [Journal Article] Total laparoscopic conservative surgery for an intramural ectopic pregnancy (2010)
  • Author(s): Nabeshima H, Nishimoto M, Utsunomiya H, Arai M, Ugajin T, Terada Y, Yaegashi N
  • Journal Title: Diagn Ther Endosc
  • Peer Reviewed
• [Journal Article] Laparoscopic management of recurrent rupture of an adnexal mass in the second trimester of pregnancy (2010)
  • Author(s): Hayasaka S, Murakami T, Nabeshima H, Terada Y, Yaegashi N
  • Journal Title: A case report Asian Journal of Endoscopic Surgery Volume: 3 Pages: 201-203
  • Peer Reviewed
• [Journal Article] The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function (2010)
  • Author(s): UgajinT, Terada Y, Hasegawa H, Nabeshima H, Suzuki K, Yaegashi N
  • Journal Title: Assist Reprod Genetics Volume: 27(2-3) Pages: 75-81
  • Peer Reviewed
• [Journal Article] Aberrant behavior of mouse embryo development after blastomere biopsy as observed through time-lapse cinematography (2010)
  • Author(s): UgajinT, Terada Y, Hasegawa H, Velayo CL, Nabeshima H, Yaegashi N
  • Journal Title: Fertil Steril Volume: 93 Pages: 2723-2728
  • Peer Reviewed
• [Journal Article] Laparoscopic removal of the rudimental uterus followed by colpopoiesis in a Mayer-Rokitansky-Kuster-Hauser syndrome patient with a functional endometrium and peritoneal endometriosis (2010)
  • Author(s): Terada Y, Ugajin T, Arai-Kikuchi M, Hayasaka S, Nabeshima H, Yaegashi N
  • Journal Title: J Gynecol Surg Volume: 26 Pages: 201-203
  • Peer Reviewed
• [Journal Article] Essential roles of the sperm centrosome in human fertilization : developing the therapy for fertilization failure due to sperm centrosomal dysfunction (2010)
  • Author(s): Terada Y, Schatten G, Hasegawa H, Yaegashi N
  • Journal Title: Tohoku J Exp Med Volume: 220(4) Pages: 247-258
  • NAID: 10027083694
  • Peer Reviewed
• [Journal Article] Establishment of Long-Term Model throughout Regular Menstrual Cycles in Immunodeficient Mice (2010)
  • Author(s): Kikuchi-Arai M, Murakami T, Utsunomiya H, Akahira JI, Suzuki-Kakisaka H, Terada Y, Tachibana M, Hayasaka S, Ugajin T, Yaegashi N
  • Journal Title: Am J Reprod Immunol Volume: 64(5) Pages: 324-332
  • Peer Reviewed
• [Journal Article] A novel culture system for mouse spermatid maturation which produces elongating spermatids capable of inducing calcium oscillation during fertilization and embryonic development (2010)
  • Author(s): Hasegawa H, Terada Y, Ugajin T, Yaegashi N, Sato K
  • Journal Title: J Assist Reprod Genet Volume: 27(9-10) Pages: 565-570
  • Peer Reviewed
• [Journal Article] Laparoscopic management of recurrent rupture of an adnexal mass in the second trimester of pregnancy : A case report (2010)
  • Author(s): Hayasaka S, Murakami T, Nabeshima H, Terada Y, Yaegashi N
  • Journal Title: Asian Journal of Endoscopic Surgery Volume: 3 Pages: 201-203
  • Peer Reviewed
• [Journal Article] The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function. (2010)
  • Author(s): Ugajin T, Terada Y, Hasegawa H, Nabeshima H, Suzuki K, Yaegashi N
  • Journal Title: Assist Reprod Genetics Volume: 27(2-3) Pages: 75-81
  • Peer Reviewed
• [Journal Article] Aberrant behavior of mouse embryo development after blastomerebiopsy as observed through time-lapse cinematography. (2010)
  • Author(s): Ugajin T, Terada Y, Hasegawa H, Velayo CL, Nabeshima H, Yaegashi N
  • Journal Title: Fertil Steril Volume: 93 Pages: 2723-2728
  • Peer Reviewed
• [Journal Article] Laparoscopic removal of the rudimental uterus followed by colpopoiesis in a Mayer-Rokitansky-Kuster-Hauser syndrome patiellt with a functional endometrium and peritoneal endometriosis. (2010)
  • Author(s): Terada Y, Ugajin T, Arai-Kikuchi M, Hayasaka S, Nabeshima H, Yaegashi N
  • Journal Title: J Gynecol Surg Volume: 26 Pages: 201-203
  • Peer Reviewed
• [Journal Article] Essential roles of the sperm centrosome in human fertilization : developing the therapy for fertilization failure due to sperm centrosomal dysfunction. (2010)
  • Author(s): Terada Y, Schatten G, Hasegawa H, Yaegashi N
  • Journal Title: Tohoku J Exp Med. Volume: 220(4) Pages: 247-258
  • NAID: 10027083694
  • Peer Reviewed
• [Journal Article] Establishment of Long-Term Model throughout Regular Menstrual Cycles in Immunodeficient Mice. (2010)
  • Author(s): Kikuchi-Arai M, Murakami T, Utsunomiya H, Akahira JI, Suzuki-Kakisaka H, Terada Y, Tachibana M, Hayasaka S, Ugajin T, Yaegashi N
  • Journal Title: Am J Reprod Immunol. Volume: 64(5) Pages: 324-332
  • Peer Reviewed
• [Journal Article] A novel culture system for mouse spermatid maturation which produces elongating spermatids capable of inducing calcium oscillation during fertilization and embryonic development. (2010)
  • Author(s): Hasegawa H, Terada Y, Ugajin T, Yaegashi N, Sato K
  • Journal Title: J Assist Reprod Genet. Volume: 27(9-10) Pages: 565-570
  • Peer Reviewed
• [Journal Article] Laparoscopic removal of the rudimental uterus followed by colpopoiesis in a Mayer-Rokitansky-Kuster-Hauser syndrome patient with a functional endometrium and peritoneal endometriosis. (2010)
  • Author(s): Terada Y, Ugajin T, Arai-Kikuchi M, Hayasaka S, Nabeshima H, Yaegashi N
  • Journal Title: J Gynecol Surg (印刷中)
  • Peer Reviewed
• [Journal Article] Laparoscopic management of recurrent rupture of an adnexal mass in the second trimester of pregnancy : A case report (2010)
  • Author(s): Hayasaka S, Murakami T, Nabeshima H, Terada Y, Yaegashi N
  • Journal Title: Asian Journal of Endoscopic (印刷中)
  • Peer Reviewed
• [Journal Article] A Method for Safe Hysteroscopic Synechiolysis in Patients with Asherman Syndrome. (2010)
  • Author(s): Hayasaka S, Murakami T, Arai M, Ugajin T, Nabeshima H, Yuki H, Terada Y, Yaegashi N
  • Journal Title: J Gyncol Surg (印刷中)
  • Peer Reviewed
• [Journal Article] A Method for Safe Hysteroscopic Synechiolysis in Patients with Asherman Syndrome (2009)
  • Author(s): Hayasaka S, Murakami T, Arai M, Ugajin T, Nabeshima H, Yuki H, Terada Y, Yaegashi N
  • Journal Title: J Gyncol Surg Volume: 25 Pages: 147-52
  • Peer Reviewed
• [Presentation] Post ICSI events in fertilizationに関わる配偶子の質について (2010)
  • Author(s): 寺田幸弘
  • Organizer: 日本生殖医学会RMBシンポジウム
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-20
• [Presentation] Post ICSI events in fertilizationに関わる配偶子の質について (2010)
  • Author(s): 寺田幸弘
  • Organizer: 日本生殖医学会 RMBシンポジウム
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-20