| Project/Area Number |
21592086
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
HAYASAKA Shinichi 東北大学, 大学院・医学系研究科, 非常勤講師 (00535099)
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| Co-Investigator(Kenkyū-buntansha) |
TERADA Yukihiro 秋田大学, 大学院・医学系研究科, 教授 (10260431)
TAKANO Tadao 東北大学, 未来医工学治療開発センター, 准教授 (40282058)
OTSUKI Takeo 東北大学, 病院, 助教 (40531330)
NABESHIMA Hiroshi 東北大学, 病院, 助教 (90547415)
HASEGAWA Hisataka 東北大学, 病院, 技能補佐員 (20455835)
IMAI Noriaki 東北大学, 大学院・医学系研究科, 非常勤講師 (60531332)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 円形精子細胞 / 精子中心体 / 補助活性化法 / 受精障害 / 生殖補助技術 / 受精能 / 発生能 / 不妊症 |
|---|
| Research Abstract |
The goal of the fertilization assumes he fusion of the sex genome in the egg cytoplasm. We examined fertilization establishment ability of the round spermatid of the mammal that was assumed to be extremely low, using human and a laboratory animal (a mouse, rabbit). Sperm function to accomplish a major event of the fertilization after the sperm entry, oocyte activation ability and sperm centrosomal function were found to be decreased. Furthermore, as one of the methods to supplement ability for oocyte activation that decreased with round spermatid, we developed the co-culture system with hormone addition and Sertoli cells of the mouse round spermatid.
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