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Melatonin protects oocyte and granulosa cellsfrom reactive oxygen species during ovulation process within the follicle

Research Project

Project/Area Number 21592099
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionYamaguchi University

Principal Investigator

TAMURA Hiroshi  山口大学, 医学部附属病院, 准教授 (50379947)

Co-Investigator(Kenkyū-buntansha) TAKETANI Toshiaki  山口大学, 医学部附属病院, 助教 (70464328)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsメラトニン / 活性酸素 / 卵子 / 顆粒膜細胞 / 酸化ストレス / 卵 / 卵胞
Research Abstract

Reactive oxygen species(ROS) are produced within the fbllicle especially during the ovulatory process. We examined whether melatonin acts as an anti-oxidant within follicles and protects oocytes and granulosa cells from oxidative stress. Melatonin, secreted by the pineal gland, is taken up into thefollicular fluid from the blood. ROS produced within the follicles, especially during the ovulation process, were scavenged by melatonin, and reduced oxidative stress may be involved in oocyte maturation, embryo development and luteinaization of granulosa cells. Our clinical study demonstrated that melatonin treatment for infertile women increases intra-fbllicular melatonin concentrations, reduces intra-follicular oxidative damage and elevates fertilization and pregnancy rates. Melatonin treatment also improves progesterone production by corpus luteum in infertile women with luteal phase defect.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (23 results)

All 2012 2011 2010 2009

All Journal Article (13 results) (of which Peer Reviewed: 11 results) Presentation (9 results) Book (1 results)

  • [Journal Article] The role of melatonin as an antioxidant in the fbllicle2012

    • Author(s)
      Tamura H, Takasaki A, Taketani T, Tanabe M, Kizuka F, Lee L, Tamura I, Maekawa R, Asada H, Yamagata Y, Sugino
    • Journal Title

      N. J Ov Res

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of melatonin as an antioxidant in the follicle2012

    • Author(s)
      Hiroshi Tamura
    • Journal Title

      Journal of Ovarian Research

      Volume: (In press) Issue: 1 Pages: 5-5

    • DOI

      10.1186/1757-2215-5-5

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protective role ofmelatonin in progesterone production by human luteal cells2011

    • Author(s)
      Taketani T, Tamura H, Takasaki A, Lee L, Kizuka F, Tamura I, Taniguchi K, Maekawa R, Asada H, Shimamura K, Reiter RJ
    • Journal Title

      Sugino N. J Pineal Res

      Volume: 51 Pages: 207-13

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential effects of progesterone on COX-2 and Mn-SOD expressions are associated with histone acetylation status ofthe promoter region in human endometrial stromal cells2011

    • Author(s)
      Tamura I, Taketani T, Lee L, Kizuka F, Taniguchi K, Maekawa R, Asada H, Tamura H
    • Journal Title

      Sugino N. J Clin Endocrinol Metab

      Volume: 96 Pages: 1073-82

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Luteal blood flow in patients undergoing GnRH agonist long protocol2011

    • Author(s)
      Takasaki A, Tamura I, Kizuka F, Lee L, Maekawa R, Asada H, Taketani T, Tamura H, Shimamura K, Morioka H, Sugino N
    • Journal Title

      J Ovarian Res

      Volume: 4 Pages: 2-2

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protective role of melatonin in progesterone production by human luteal cells2011

    • Author(s)
      Toshiaki Taketani
    • Journal Title

      Journal of Pineal Research

      Volume: 51 Pages: 207-213

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生殖機能調節の新しい視点10.メラトニンの卵巣機能調節2010

    • Author(s)
      田村博史、杉野法広
    • Journal Title

      産科と婦人科

      Volume: 第77巻3号 Pages: 310-316

    • Related Report
      2011 Final Research Report
  • [Journal Article] Prostaglandin F2α(PGF2α) stimulates PTGS2 expression and PGF2αsynthesis through NFKB activation via reactive oxygen species in the corpus luteum of pseudopregnant rats2010

    • Author(s)
      Sugino N. Reproduction.
    • Journal Title

      Sugino N. Reproduction

      Volume: 140(6) Pages: 885-892

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Progesterone increases manganese superoxide dismutase expression via a cAMP-dependent signaling mediated by noncanonical Wnt5a pathway in human endometrial stromal cells2010

    • Author(s)
      Matsuoka A, Kizuka F, Lee L, Tamura I, Taniguchi K, Asada H, Taketani T, Tamura H
    • Journal Title

      Sugino N. J Clin Endocrinol Metab

      Volume: 95 Pages: 291-299

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 卵過程におけるメラトニンの防御的役割-メラトニンは卵胞内で卵と顆粒膜細胞を酸化ストレスから護る-2009

    • Author(s)
      田村博史
    • Journal Title

      日本産科婦人科学会誌

      Volume: 第61巻第10号 Pages: 1925-1934

    • Related Report
      2011 Final Research Report
  • [Journal Article] Melatonin and the ovary ; physiological and pathophysiological implications.[ Review article]2009

    • Author(s)
      Tamura H, Nakamura Y, Korkmaz A, Manchester LC, Tan DX, Norihiro S
    • Journal Title

      Reiter RJ. Fertil Steril

      Volume: 92 Pages: 328-343

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Combination of melatonin and a peroxisome proliferator-activated receptor-gamma agonist induces apoptosis in a breast cancer cell line2009

    • Author(s)
      Korkmaz A, Tamura H, Manchester LC, Ogden GB, Tan DX
    • Journal Title

      Reiter RJ. J Pineal Res

      Volume: 46 Pages: 115-116

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Melatonin and the ovary ; physiological and pathophysiological implications.2009

    • Author(s)
      HIROSHI TAMURA
    • Journal Title

      Fertil Steril 92

      Pages: 328-343

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] -メラトニン研究の最近の進歩と医療への応用-パネルディスカッション『私のメラトニン使用経験から』2011

    • Author(s)
      田村博史
    • Organizer
      第3回抗加齢内分泌研究会学術集会
    • Place of Presentation
      横浜市鶴見大学会館
    • Year and Date
      2011-09-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 松果体ホルモンであるメラトニンは卵胞内で卵や穎粒膜細胞を護るシンポジウム9「生殖機能の中枢性調節:最近の知見」2011

    • Author(s)
      田村博史
    • Organizer
      第84回日本内分泌学会学術総会
    • Place of Presentation
      神戸市神戸国際会議場
    • Year and Date
      2011-04-23
    • Related Report
      2011 Final Research Report
  • [Presentation] 松果体ホルモンであるメラトニンは卵胞内で卵や顆粒膜細胞を護る2011

    • Author(s)
      田村博史
    • Organizer
      第84回日本内分泌学会学術総会シンポジウム9
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2011-04-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Influence of oxidative stress on oocyte quality and protective role of melatonin as an antioxidant2010

    • Author(s)
      Hiroshi Tamura, Toshiaki Taketani, Akihisa Takasaki, Norihiro Sugino
    • Organizer
      IFFS 2010 20^<th> World Congress on Fertility and Sterility
    • Place of Presentation
      Munich, Germany
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 松果体ホルモンメラトニンと生殖2009

    • Author(s)
      田村博史
    • Organizer
      第27回内分泌代謝学サマーセミナー
    • Place of Presentation
      福井県三国観光ホテル
    • Year and Date
      2009-07-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Melatonin protects granulosa cells for progesterone production as an antioxidant in human ovarian follicles2009

    • Author(s)
      TOSHIAKI TAKETANI
    • Organizer
      25^<th> European Society of Human Reproduction and Embryology
    • Place of Presentation
      Amsterdam, The Netherlands
    • Year and Date
      2009-06-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 松果体ホルモンメラトニンと生殖2009

    • Author(s)
      田村博史
    • Organizer
      第82回日本内分泌学会研究奨励賞受賞講演
    • Place of Presentation
      前橋市群馬県民会館
    • Year and Date
      2009-04-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 排卵過程におけるメラトニンの防御的役割-メラトニンは卵胞内で卵と顆粒膜細胞を酸化ストレスから護る-2009

    • Author(s)
      田村博史
    • Organizer
      第61回日本産科婦人科学会学術講演会シンポジウム2『中枢神経関連生理活性物質の生殖機能へのかかわり』
    • Place of Presentation
      京都市国立京都国際会館
    • Year and Date
      2009-04-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Melatonin protects oocyte and granulosa cells from reactive oxygen species(ROS) during the ovulatory process2009

    • Author(s)
      Hiroshi Tamura, Toshiaki Taketani, Norihiro Sugino
    • Organizer
      XI Congress of the European Biological Rhythms Society
    • Place of Presentation
      Strasbourg, France
    • Related Report
      2011 Final Research Report
  • [Book] 産科と婦人科 生殖機能調節の新しい視点 メラトニンの卵巣機能調節2010

    • Author(s)
      田村博史
    • Total Pages
      7
    • Publisher
      診断と治療社
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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