Cadherin-mediated reconstitution mechanism of human endometrial epithelial cells during implantation研究代表者:

• Project/Area Number: 21592108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: UCHIDA Hiroshi 慶應義塾大学, 医学部, 助教 (90286534)
• Co-Investigator(Kenkyū-buntansha): MARUYAMA Tetsuo 慶應義塾大学, 医学部, 講師 (10209702)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 生殖医学 / 着床 / カドヘリン / 上皮間充織転換 / 組織再構築

Research Abstract

Human endometrial epithelial cell(EEC) layer, which is structural and immunological barrier, is temporally disrupted by penetration of fertilized egg in the process of pregnancy, however the reconstruction mechanism of disrupted EEC layer is largely unknown. This study demonstrated that epithelial mesenchymal transition is involved in the mechanism in association with redistribution of N-cadherin. These results are useful for the new strategy of assisted reproductive technology.

Research Products

• [Journal Article] Involvement of epithelial mesenchymal transition of human endometrial epithelial cells in human embryo implantation : evidence from studies using in vitro model (2012)
  • Author(s): Uchida H, Maruyama T, Nishikawa-Uchida S, Oda H, Miyazaki K, Yamasaki A, Yoshimura Y
  • Journal Title: J Biol Chem Volume: 287 Pages: 4441-4450
• [Journal Article] Psychological adjustment and psychosocial stress among Japanese couples with a history of recurrent pregnancy loss (2012)
  • Author(s): Kagami M, Maruyama T, Koizumi T, Miyazaki K, Nishikawa-Uchida S, Oda H, Uchida H, Fujisawa D, Ozawa N, Schmidt L, Yoshimura Y
  • Journal Title: Hum Reprod Volume: 27 Pages: 787-794
  • Peer Reviewed
• [Journal Article] Involvement of epithelial mesenchymal transition of human endometrial epithelial cells in human embryo implantation : evidence from studies using in vitro model (2012)
  • Author(s): H.Uchida, T.Maruyama, S.Nishikawa-Uchida, H.Oda, K.Miyazaki, A.Yamasaki, Y.Yoshimura
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 7 Pages: 4441-4450
  • DOI: 10.1074/jbc.m111.286138
  • Peer Reviewed
• [Journal Article] Psychological adjustment and psychosocial stress among Japanese couples with a history of recurrent pregnancy loss (2012)
  • Author(s): M.Kagami, T.Maruyama, T.Koizumi, K.Miyazaki, S.Nishikawa-Uchida, H.Oda, H.Uchida, D.Fujisawa, N.Ozawa, L.Schmidt, Y.Yoshimura
  • Journal Title: Human Reproduction Volume: 27 Issue: 3 Pages: 787-794
  • DOI: 10.1093/humrep/der441
  • Peer Reviewed
• [Journal Article] OCT4 expression in human uterine myometrial stem/progenitor cells (2010)
  • Author(s): Ono M, Kajitani T, Uchida H, Arase T, Oda H, Nishikawa-Uchida S, Masuda H, Nagashima T, Yoshimura Y, Maruyama T
  • Journal Title: Hum Reprod Volume: 25 Pages: 2059-2067
  • Peer Reviewed
• [Journal Article] Stem cell-like properties of the endometrial side population : implication in endometrial regeneration (2010)
  • Author(s): Masuda H, Matsuzaki Y, Hiratsu E, Ono M, Nagashima T, Kajitani T, Arase T, Oda H, Uchida H, Asada H, Ito M, Yoshimura Y, Maruyama T, Okano H
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] Stem cell-like properties of the endometrial side population : implication in endometrial regeneration. (2010)
  • Author(s): Masuda H, Matsuzaki Y, Hiratsu E, Ono M, Nagashima T, Kajitani T, Arase T, Oda H, Uchida H, Asada H, Ito M, Yoshimura Y, Maruyama T, Okano H
  • Journal Title: PLoS One. Volume: 5
  • Peer Reviewed
• [Journal Article] OCT4 expression in human uterine myometrial stem/progenitor cells. (2010)
  • Author(s): Ono M, Kajitani T, Uchida H, Arase T, Oda H, Nishikawa-Uchida S, Masuda H, Nagashima T, Yoshimura Y, Maruyama T
  • Journal Title: Hum Reprod. Volume: 25 Pages: 2059-2067
  • Peer Reviewed
• [Journal Article] Epigenetic Treatment in Assisted Human Embryo Implantation (2009)
  • Author(s): Uchida H, Maruyama T, Kagami M, Oda H, Nishikawa S, Kajitani T, Asada H, Yoshimura Y
  • Journal Title: J Mam Ova Res Volume: 26 Pages: 116-121
  • NAID: 130004436751
  • Peer Reviewed
• [Journal Article] The UDP-glucose receptor P2RY14 triggers innate mucosal immunity in the female reproductive tract by inducing IL-8 (2009)
  • Author(s): Arase T, Uchida H, Kajitani T, Ono M, Tamaki K, Oda H, Nishikawa S, Kagami M, Nagashima T, Masuda H, Asada H, Yoshimura Y, Maruyama T
  • Journal Title: J Immunol Volume: 182 Pages: 7074-7084
  • Peer Reviewed
• [Journal Article] Premature ovarian failure and androgen receptor gene CAG repeat lengths weighted by X chromosome inactivation patterns (2009)
  • Author(s): Sugawa F, Wada Y, Maruyama T, Uchida H, Ishizuka B, Ogata T
  • Journal Title: Fertil Steril Volume: 91 Pages: 649-652
  • Peer Reviewed
• [Journal Article] Epigenetic Treatment in Assisted Human Embryo Implantation (2009)
  • Author(s): Uchida H, Maruyama T, Kagami M, Oda H, Nishikawa S, Kajitani T, Asada H, Yoshimura Y
  • Journal Title: J.Mamm.Ova Res 26 Pages: 116-121
  • NAID: 130004436751
  • Peer Reviewed
• [Journal Article] The UDP-glucose recepter P2RY14 triggers innate mucosal immunity in the female reproductive tract by inducing IL-8 (2009)
  • Author(s): Arase T, Uchida H, Kajitani T, Ono M, Tamaki K, Oda H, Nishikawa S, Kagami M, Nagashima T, Masuda H, Asada H, Yoshimura Y, Maruyama T
  • Journal Title: J Immunol 182 Pages: 7074-7084
  • Peer Reviewed
• [Journal Article] Premature ovarian failure and androgen receptor gnen CAG repeat lengths weighted by X chromosome inactivation patterns (2009)
  • Author(s): Sugawa F, Wada Y, Maruyama T, Uchida H, Ishizuka B, Ogata T
  • Journal Title: Fertil Steril 91 Pages: 649-652
  • Peer Reviewed
• [Presentation] ヒト着床における子宮内膜上皮の細胞死によらない新たな胚陥入促進機序-N-cadherin依存性細胞運動- (2011)
  • Author(s): 内田浩, 丸山哲夫, 内田明花, 山崎彰子, 宮崎薫, 吉村泰典
  • Organizer: 第16回日本生殖内分泌学会学術総会
  • Year and Date: 2011-11-19
• [Presentation] ヒト着床における子宮内膜上皮の細胞死によらない新たな胚陥入促進機序-N-cadher in依存性細胞運動- (2011)
  • Author(s): 内田浩
  • Organizer: 第16回日本生殖内分泌学会学術総会
  • Place of Presentation: 東京都千代田区・シェーンバッハ・サボー
  • Year and Date: 2011-11-19
• [Presentation] Epithelial-mesenchymal transition mediates the displacement of endometrial epithelial cells away from the site of embryo implantation. (2011)
  • Author(s): Uchida H, Maruyama T, Uchida-Nishikawa, S, Miyazaki K, Yoshimura Y
  • Organizer: World Congress of Reproductive Biology.
  • Place of Presentation: Cairns, Australia
• [Presentation] ヒト着床モデルにおける胚伸展のための子宮内膜上皮動態の分子機序解析-Rho family低分子量Gタンパク質の機能関与- (2011)
  • Author(s): 内田浩, 丸山哲夫, 内田明花, 小田英之, 宮崎薫, 各務真紀, 青木大輔, 吉村泰典
  • Organizer: 第63回日本産科婦人科学会総会・学術講演会
• [Presentation] ヒト着床における子宮内膜上皮細胞動態-卵巣ホルモンと低分子量Gタンパク質の関与- (2011)
  • Author(s): 内田浩, 丸山哲夫, 西川明花, 小田英之, 宮崎薫, 各務真紀, 吉村泰典
  • Organizer: 第84回日本内分泌学会学術総会
• [Presentation] Epithelial-mesenchymal transition mediates the displacement of endometrial epithelial cells away from the site of embryo implantation (2011)
  • Author(s): H.Uchida
  • Organizer: 2011 World Congress of Reproductive Biology
  • Place of Presentation: Cairns, Australia
• [Presentation] ヒト着床における子宮内膜上皮細胞動態-卵巣ホルモンと低分子量Gタンパク質の関与- (2011)
  • Author(s): 内田浩
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 兵庫県神戸市・神戸国際会議場
• [Presentation] ヒト着床モデルにおける胚伸展のための子宮内膜上皮動態の分子機序解析-Rho family低分子量Gタンパク質の機能関与- (2011)
  • Author(s): 内田浩
  • Organizer: 第63回日本産科婦人科学会総会・学術講演会
  • Place of Presentation: 大阪府大阪市・大阪国際会議場
• [Presentation] ヒト着床における子宮内膜腺上皮の複合的機能の分子制御 (2010)
  • Author(s): 内田浩
  • Organizer: 第62回日本産科婦人科学会学術講演会
  • Year and Date: 2010-04-24
• [Presentation] ヒト着床におけるN-cadherinによるホルモン依存的子宮内膜腺上皮細胞層の再構築 (2010)
  • Author(s): 内田浩, 丸山哲夫, 小田英之, 西川明花, 梶谷宇, 宮崎薫, 各務真紀, 吉村泰典
  • Organizer: 第83回日本内分泌学会学術総会
• [Presentation] N-Cadherin and RhoA Have Opposite Effects on the Regulation of Endometrial Epithelial Cell Migration during Implantation (2010)
  • Author(s): Uchida H, Maruyama T, Arase, T, Ono M, Kajitani, T, Kagami, M, Oda, H, Nishikawa, S, Yoshimura Y
  • Organizer: 57th Society for Gynecologic Investigation
  • Place of Presentation: Orland, Florida, USA
• [Presentation] 【シンポジウム 子宮内膜の機能調節とその病態】ヒト着床における子宮内膜腺上皮の複合的機能の分子制御 (2010)
  • Author(s): 内田浩
  • Organizer: 第62回日本産科婦人科学会
  • Place of Presentation: 東京(招請講演)
• [Presentation] ヒト着床における子宮内膜腺上皮細胞とグリコデリン (2009)
  • Author(s): 内田浩
  • Organizer: 日本ヒト細胞学会
  • Year and Date: 2009-08-22
• [Presentation] 着床時子宮内膜腺上皮のダイナミクスと分子制御 (2009)
  • Author(s): 内田浩
  • Organizer: 第27回日本受精着床学会総会・学術講演会
  • Year and Date: 2009-08-07
• [Presentation] 着床時子宮内膜腺上皮のダイナミクスと分子制御 (2009)
  • Author(s): 内田浩
  • Organizer: 第27回日本受精着床学会
  • Place of Presentation: 東京都港区・東京都慈恵医科大学
• [Presentation] 着床時子宮内膜腺上皮のダイナミクスと分子制御 (2009)
  • Author(s): 内田浩
  • Organizer: 第27回日本ヒト細胞学会
  • Place of Presentation: 京都府京都市・国立京都国際会館