Analysis of carbohydrate structures related to metastasis of gynecologic cancers and establishment of human monoclonal antibodies towards these carbohydrate structures

• Project/Area Number: 21592145
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: SUZUKI Atsushi 慶應義塾大学, 医学部, 助教 (00255514)
• Co-Investigator(Kenkyū-buntansha): TOMINAGA Eiichiro 慶應義塾大学, 医学部, 助教 (80276328) ; SUZUKI Masami 慶應義塾大学, 医学部, 共同研究員 (10276321)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 婦人科癌 / 抗体医療 / 糖鎖抗原 / モノクローナル抗体 / リンパ節転移 / ヒトモノクローナル抗体

Research Abstract

We have developed a gynecologic cancer-specific human monoclonal antibody HMOCC-1 that demonstrates anti-tumor activity towards ovarian cancer, among gynecologic cancers. The antibody has a specific carbohydrate antigen epitope that is a sulfated carbohydrate moiety. The carbohydrate antigen recognized by this antibody is a newly characterized epitope present on gynecologic cancers and its glycan structure was elucidated to be SO3→3Galβ1→4GlcNAcβ1→3(±SO3→6) Galβ1→4GlcNAcβ1→.

Research Products

• [Journal Article] Identification of mono-and di-sulfated N-acetyl-lactosaminyl oligosaccharide structures as epitopes specifically recognized by humanized monoclonal antibody HMOCC-1 raised against ovarian cancer (2012)
  • Author(s): Shibata T, Matsumura F, Wang P, Yu SY, Khoo KH, Kitayama K, Akama T, Sugihara K, Kojima-Aikawa K, Seeberger P, Fukuda M, Suzuki A, Aoki D, Fukuda MN
  • Journal Title: J. Biol Chem Volume: 287(9) Pages: 6592-6602
  • Peer Reviewed
• [Journal Article] Identification of ANXA4, PSAT1, CRABP2 and SPB5 as histology-specific biomarker candidates for ovarian cancer by proteomic characterization (2012)
  • Author(s): Toyama A, Suzuki A, Shimada T, Aoki C, Aoki Y, Umino Y, Nakamura Y, Aoki D, Sato T
  • Journal Title: Cancer Sci Volume: 103(4) Pages: 747-55
  • Peer Reviewed
• [Journal Article] Proteomic characterization of ovarian cancers identifying annexin-A4, phosphoserine aminotransferase, cellular retinoic acid-binding protein 2, and serpin B5 as histology-specific biomarkers (2012)
  • Author(s): Toyama A, Suzuki A, et al
  • Journal Title: Cancer Sci. Volume: 103(4) Pages: 747-755
  • Peer Reviewed
• [Journal Article] Identification of mono- and disulfated N-acetyl-lactosaminyl oligosaccharide structures as epitopes specifically recognized by humanized monoclonal antibody HMOCC-1 raised against ovarian cancer (2012)
  • Author(s): Shibata TK, Matsumura F, Suzuki A, et al
  • Journal Title: J Biol.Chem. Volume: 287(9) Issue: 9 Pages: 6592-6602
  • DOI: 10.1074/jbc.m111.305334
  • Peer Reviewed
• [Journal Article] HNK-1 glycan functions as a tumor suppressor for astrocytic tumor (2011)
  • Author(s): Suzuki-Anekoji M, Suzuki M, Kobayashi T, Sato Y, Nakayama J, Suzuki A, Bao X, Angata K, Fukuda M
  • Journal Title: J. Biol Chem Volume: 286(37) Pages: 32824-33
  • Peer Reviewed
• [Journal Article] The chemosensitivity of nodal metastases in recurrent epithelial ovarian cancer (2011)
  • Author(s): Kataoka F, Tsuda H, Nomura H, Chiyoda T, Tominaga E, Suzuki A, Susumu N, Aoki D
  • Journal Title: Eur J Gynaecl Oncol Volume: 32(2) Pages: 160-3
  • Peer Reviewed
• [Journal Article] The chemosensitivity of nodal metastases in recurrent epithelial ovarian cancer (2011)
  • Author(s): Kataoka F, Tsuda H, Suzuki A, et al
  • Journal Title: Eur J.Gynaecol.Oncol. Volume: 32(2) Pages: 160-163
  • Peer Reviewed
• [Journal Article] HNK-1 glycan functions as a tumor suppressor for astrocytic tumor (2011)
  • Author(s): Suzuki-Anekoji M, Suzuki M, Suzuki A, et al
  • Journal Title: J Biol.Chem. Volume: 286(37) Pages: 32824-32833
  • Peer Reviewed
• [Journal Article] Effects of third-line chemotherapy for women with recurrent ovarian cancer who received platinum/taxane regimens as first-line chemotherapy (2010)
  • Author(s): Chiyoda T, Tsuda H, Nomura H, Kataoka F, Tominaga E, Suzuki A, Susumu N, Aoki D
  • Journal Title: Eur J Gynaecol Oncol Volume: 31(4) Pages: 364-8
  • Peer Reviewed
• [Journal Article] Lymph node metastasis in grossly apparent stages I and II epithelial ovarian cancer (2010)
  • Author(s): Nomura H, Tsuda H, Susumu N, Fujii T, Banno K, Kataoka F, Tominaga E, Suzuki A, Chiyoda T, Aoki D
  • Journal Title: Int J Gynecol Cancer Volume: 3 Pages: 341-5
  • Peer Reviewed
• [Journal Article] The sensitivity and specificity of a new formula to distinguish endometrioid type endometrial carcinoma from ovarian endometrial carcinoma (2010)
  • Author(s): Nishimura S, Ito YM, Tsuda H, Ohnishi Y, Kataoka F, Nomura H, Chiyoda T, Suzuki A, Susumu N, Aoki D, Hatae M
  • Journal Title: Eur J Obstet Gynecol Reprod Biol Volume: 148(1) Pages: 67-72
  • Peer Reviewed
• [Journal Article] Amplification of GNAS may be an independent, qualitative and reproducible biomarker to predict progression-free survivial in epithelial ovarian cancer (2010)
  • Author(s): Tominaga E, Tsuda H, Arao T, Nishimura S, Takano M, Kataoka F, Nomura H, Hirasawa A, Aoki D, Nishio K
  • Journal Title: Gynecol. Oncol Volume: 118(2) Pages: 160-6
  • Peer Reviewed
• [Journal Article] Effects of third-line chemotherapy for women with recurrent ovarian cancer who received platinum/taxane regimens as first-line chemotherapy (2010)
  • Author(s): Chiyoda T, Tsuda H, Suzuki, A, et al.
  • Journal Title: Eur J.Gynaecol.Oncol. Volume: 31(4) Pages: 364-368
  • Peer Reviewed
• [Journal Article] Lymph node metastasis in grossly apparent stages I and II epithelial ovarian cancer (2010)
  • Author(s): Nomura H, Tsuda H, Suzuki, A, et al.
  • Journal Title: Int.J.Gynecol.Cancer Volume: 20(3) Pages: 341-345
  • Peer Reviewed
• [Journal Article] Amplification of GNAS may be an independent, qualitative and reproducible biomarker to predict progression-free survivial in epithelial ovarian cancer (2010)
  • Author(s): Tominaga E, Tsuda H, et al.
  • Journal Title: Gynecol.Oncol. Volume: 118 Pages: 160-166
  • Peer Reviewed
• [Journal Article] The sensitivity and specificity of a new formula to distinguish endometroid type endometrial carcinoma from ovarian endometrial carcinoma (2010)
  • Author(s): Nishimura, S., Suzuki, A, et al.
  • Journal Title: Eur J.Obstet Gynecol.Reprod Biol 148(1) Pages: 67-72
  • Peer Reviewed
• [Presentation] Novel function of HNK-1 sulfotransferase as a hormone regulator (2011)
  • Author(s): Suzuki-Anekoji M
  • Organizer: Annual Meeting for the Society for Glycobiology
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2011-11-10
• [Presentation] HNK-1 glycan functions as a tumor suppressor for astrocytic tumor (2011)
  • Author(s): Suzuki-Anekoji M
  • Organizer: Annual Meeting for the Society for Glycobiology
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2011-11-10
• [Presentation] Novel function of HNK-1 sulfotransferase as a hormone regulator (2011)
  • Author(s): Suzuki-Anekoji M(共同演者:鈴木淳)
  • Organizer: Annul Meeting for the Society for Glycobiology
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2011-11-10
• [Presentation] HNK-1 glycan functions as a tumor suppressor for astrocytic tumor (2011)
  • Author(s): Suzuki-Anekoji M(共同演者:鈴木淳)
  • Organizer: Annul Meeting for the Society for Glycobiology
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2011-11-10
• [Presentation] 卵巣癌の各組織型におけるANXA4, SPB5, CRABP2, PST1分子発現とバイオマーカーとしての有用性 (2011)
  • Author(s): 遠山敦彦
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 国際会議場、名古屋
  • Year and Date: 2011-10-03
• [Presentation] 卵巣癌の各組織型におけるANXA4,SPB5,CRABP2,PST1分子発現とバイオマーカーとしての有用性 (2011)
  • Author(s): 遠山敦彦(共同演者:鈴木淳)
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-03
• [Presentation] 卵巣癌の組織型特異的なバイオマーカーの開発と血清診断の可能性 (2011)
  • Author(s): 遠山敦彦
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ、横浜
  • Year and Date: 2011-10-01
• [Presentation] 卵巣癌間質の遺伝子解析を用いた新規バイオマーカーの検索ー癌間質のEGR-1発現は上皮性卵巣癌の予後と相関する (2011)
  • Author(s): 片岡史夫
  • Organizer: 第63回日本産婦人科学会総会学術講演会
  • Place of Presentation: 国際会議場、大阪
  • Year and Date: 2011-08-30
• [Presentation] シアリダーゼ発現低下は卵巣癌腹膜播種を抑制する (2011)
  • Author(s): 野村弘行
  • Organizer: 第63回日本産婦人科学会総会学術講演会
  • Place of Presentation: 国際会議場、大阪
  • Year and Date: 2011-08-30
• [Presentation] 卵巣癌間質の遺伝子解析を用いた新規バイオマーカーの検索-癌間質のEGR-1発現は上皮性卵巣癌の予後と相関する (2011)
  • Author(s): 片岡史夫(共同演者:鈴木淳)
  • Organizer: 第63回日本産婦人科学会総会学術講演会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2011-08-30
• [Presentation] シアリダーゼ発現低下は卵巣癌腹膜播種を抑制する (2011)
  • Author(s): 野村弘行(共同演者:鈴木淳)
  • Organizer: 第63回日本産婦人科学会総会学術講演会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2011-08-30
• [Presentation] 絨毛癌におけるSOX遺伝子群の発現検索と新規治療標的の可能性 (2011)
  • Author(s): 冨永英一郎
  • Organizer: 第50回日本婦人科腫瘍学会学術講演会
  • Place of Presentation: 札幌コンベンションセンター、(北海道)
  • Year and Date: 2011-07-23
• [Presentation] ヒトモノクローナル抗体HMMC-1は癌の増殖に対して抑制的に作用する (2010)
  • Author(s): 笈川文子
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学学会
  • Place of Presentation: 神戸ポートアイランド、神戸
  • Year and Date: 2010-12-07
• [Presentation] Fertility-preserving high-dose medroxy-progesterone acetate(MPA) therapy for 4 young patients with grade 2 endometrioid adenocarcinoma (2010)
  • Author(s): 進伸幸
  • Organizer: 13^<th> Bienniel Meeting of the International Gynecologic Cancer Society
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2010-10-24
• [Presentation] Fertility-preserving high dose medroxyprogesterone acetate (MPA) therapy for 4 young Patient with grade 2 endometrioid adencarcinoma (2010)
  • Author(s): 進伸幸(共同演者:鈴木淳)
  • Organizer: 13^<th> Bienniel Meeting of the International Gynecologic Cancer Society
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2010-10-24
• [Presentation] Expression of ANXA4, SPB5, CRABP2, and PSAT1 in ovarian cancer histological subtypes used for diagnostic purposes (2010)
  • Author(s): 遠山敦彦
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 国際会議場、大阪
  • Year and Date: 2010-09-23
• [Presentation] 標準治療を施行した上皮性卵巣癌を規定する遺伝子の探索 (2010)
  • Author(s): 冨永英一郎
  • Organizer: 第9回日本婦人科がん分子標的研究会
  • Place of Presentation: 大津プリンスホテル、滋賀
  • Year and Date: 2010-09-10
• [Presentation] GNAS遺伝子の増幅は標準治療を施行した上皮性卵巣癌の独立した予後因子である (2010)
  • Author(s): 冨永英一郎
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム、東京
  • Year and Date: 2010-04-23
• [Presentation] 子宮体癌細胞が発現している糖タンパク質と転移性の関連 (2009)
  • Author(s): 笈川文子
  • Organizer: Glyco Tokyo 2009シンポジウム
  • Place of Presentation: お茶の水大学、東京
  • Year and Date: 2009-11-14
• [Presentation] Lymph node metastasis in grossly stage I and II epithelial ovarian cancer (2009)
  • Author(s): 野村弘行
  • Organizer: 16^<th> International Meeting of the European Society of Gynecologic Oncology
  • Place of Presentation: Belgrade, Serbia
  • Year and Date: 2009-11-12
• [Presentation] 卵巣癌の組織型特異的なバイオマーカーの開発と血清診断の可能性 (2009)
  • Author(s): 遠山敦彦(共同演者:鈴木淳)
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ、横浜
  • Year and Date: 2009-10-01
• [Presentation] 標準治療を受けた表層上皮性卵巣癌の再発リスクを規定する遺伝子の検索 (2009)
  • Author(s): 冨永英一郎
  • Organizer: 第61回日本産科婦人科学術集会
  • Place of Presentation: 国際会議場、京都
  • Year and Date: 2009-04-04