The Relationship between Aging and Heat Shock Response in the Inner Ear

• Project/Area Number: 21592157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Yamaguchi University
• Principal Investigator: YAMASHITA Hiroshi 山口大学, 大学院・医学系研究科, 教授 (00210419)
• Co-Investigator(Kenkyū-buntansha): SHIMOGORI Hiroaki 山口大学, 大学院・医学系研究科, 准教授 (70226273) ; SUGAHARA Kazuma 山口大学, 医学部附属病院, 講師 (20346555) ; MIKURIYA Takefumi 山口大学, 医学部附属病院, 助教 (00467797)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 内耳 / 老化 / 熱ショック蛋白質 / 加齢 / 熱ショック応答

Research Abstract

Mechanisms of age-related hearing loss have not been elucidated as aging processes are extremely complex. We examined age-related expression of HSPs in the cochlea of animal model of age-related hearing loss. The results showed that the mice showed weakening expression of Hsp70 and Hsp110 with age. In addition, we showed that hearing loss deteriorated in HSF1 null mice. These results demonstrate that the heat shock responses have a grate role in the age-related hearing loss

Research Products

• [Journal Article] Geranylgeranylacetone suppresses noise-induced expression of proinflammatory cytokines in the cochlea (2011)
  • Author(s): Nakamoto T, Mikuriya T, Sugahara K, Hirose Y, Hashimoto T, Shimogori H, Takii R, Nakai A, Yamashita H
  • Journal Title: Auris Nasus Larynx : Epub Volume: (Epub) Issue: 3 Pages: 270-274
  • DOI: 10.1016/j.anl.2011.06.001
  • Peer Reviewed
• [Journal Article] 難聴に対する鼓室注入による局所治療 (2011)
  • Author(s): 橋本誠, 山下裕司
  • Journal Title: ENTONI Volume: 132 Pages: 50-54
• [Journal Article] SSSR、FGLMNH2による有毛細胞保護効果について (2011)
  • Author(s): 吉田周平, 菅原一真, 豊田英樹, 金川英寿, 下郡博明, 山下裕司
  • Journal Title: 頭頸部自律神経 Volume: 25 Pages: 22-23
• [Journal Article] AMPAによる末梢前庭障害に対するFGLM+ SSSRの効果 (2011)
  • Author(s): 豊田英樹, 下郡博明, 菅原一真, 吉田周平, 山下裕司
  • Journal Title: 頭頸部自律神経 Volume: 25 Pages: 20-21
• [Journal Article] SSSR、FGLM-NH2による有毛細胞保護効果について (2011)
  • Author(s): 吉田周平, 他
  • Journal Title: 頭頸部自律神経 Volume: 25 Pages: 22-23
• [Journal Article] AMPAによる末梢前庭障害に対するFGLM+SSSRの効果 (2011)
  • Author(s): 豊田英樹, 他
  • Journal Title: 頭頸部自律神経 Volume: 25 Pages: 20-21
• [Journal Article] めまい急性期に対するカクテル療法 (2010)
  • Author(s): 橋本誠, 山下裕司
  • Journal Title: ENTONI Volume: 120 Pages: 8-13
• [Journal Article] 前庭神経炎 (2010)
  • Author(s): 山下裕司
  • Journal Title: CLINICAN Volume: 587 Pages: 33-37
  • NAID: 40021209070
• [Journal Article] 蝸牛における音響障害後の炎症性サイトカインの動向 (2010)
  • Author(s): 中本哲也, 他
  • Journal Title: 頭頸部自律神経 Volume: 24 Pages: 15-17
• [Journal Article] 老人性難聴における熱ショック応答の役割 (2010)
  • Author(s): 菅原一真
  • Journal Title: Otology Japan Volume: 20 Pages: 208-212
  • NAID: 10029699104
• [Journal Article] AMPAによる末梢前庭障害におけるサブスタンスPの影響 (2010)
  • Author(s): 豊田英樹, 他
  • Journal Title: 頭頸部自律神経 Volume: 24 Pages: 18-19
• [Journal Article] 熱ショック応答と内耳保護機能 (2009)
  • Author(s): 山下裕司, 御厨剛史
  • Journal Title: 耳鼻咽喉科展望 Volume: 52 Pages: 10-16
• [Journal Article] Unilateral intra-perilymphatic infusion of substance P enhances ipsilateral vestibulo-ocular reflex gains in the sinusoidal rotation test (2009)
  • Author(s): Orita H, Shimogori H, Yamashita H
  • Journal Title: Neuroscience Letters Volume: 449 Pages: 207-210
  • Peer Reviewed
• [Journal Article] 加齢における内耳熱ショック蛋白質発現の変化 (2009)
  • Author(s): 広瀬敬信, 菅原一真, 御厨剛史, 中本哲也, 橋本誠, 下郡博明, 山下裕司
  • Journal Title: 頭頸部自律神経第26回頭頸部自律神経研究会記録集 Volume: 23 Pages: 33-35
• [Journal Article] 前庭感覚細胞死におけるBcl-xLの役割 (2009)
  • Author(s): 菅原一真, 宮内裕爾, 広瀬敬信, 御厨剛史, 橋本誠, 下郡博明, 山下裕司
  • Journal Title: 頭頸部自律神経第26回頭頸部自律神経研究会記録集 Volume: 23 Pages: 30-32
• [Journal Article] ImageJを用いた眼振解析とその表示-平衡機能検査法基準化のための資料に基づいて- (2009)
  • Author(s): 池田卓生, 橋本誠, 山下裕司
  • Journal Title: Equilibrium Res Volume: 68 Pages: 92-96
  • NAID: 10026088078
• [Journal Article] 内耳疾患の治療をめざして-基礎研究の最前線-末梢前庭器保護を目的とした治療戦略- (2009)
  • Author(s): 山下裕司
  • Journal Title: 日本耳鼻咽喉科学会会報 Volume: 112 Pages: 12-17
• [Journal Article] 内耳疾患の治療をめざして-基礎研究の最前線-末梢前庭器保護を目的とした治療戦略- (2009)
  • Author(s): 山下裕司
  • Journal Title: 日本耳鼻咽喉科学会会報 112 Pages: 12-17
• [Journal Article] 熱ショック応答と内耳保護機能 (2009)
  • Author(s): 山下裕司, 御厨剛史
  • Journal Title: 耳鼻咽喉科展望 52 Pages: 10-16
• [Journal Article] (2009)
  • Author(s): 山下裕司, 菅原一真
  • Journal Title: 炎症・再生医学事典再生医学-体性幹細胞と組織修復-耳鼻咽喉(朝倉書店) Pages: 450-452
• [Presentation] Age related hearing loss in the animal model of diabetes with obesity (2012)
  • Author(s): Sugahara K, et al
  • Organizer: The 34th Annual Midwinter Meeting, ARO
  • Place of Presentation: San Diego, USA
  • Year and Date: 2012-02-26
• [Presentation] Topical ApPlication of FGLM-NH2 and SSSR Facilitates Vestibular (2012)
  • Author(s): Hideki Toyota, Hiroaki Shimogori, Kazuma Sugahara, Hiroshi Yamashita
  • Organizer: Functional Recovery Induced by AMPA 35th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2012-02-25
• [Presentation] Vestibular Research and AMPA (2012)
  • Author(s): Syuhei Yoshida, Hiroaki Shimogori, Hideki Toyota, Kazuma Sugahara, Hiroshi Yamashita
  • Organizer: 35th Annual MidWinter ResearchMeeting of the Association for Research in Otolaryngology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2012-02-25
• [Presentation] Age Related Hearing Loss in the Animal Model of Diabetes with Obesity (2012)
  • Author(s): Kazuma Sugahara, Junko Tsuda, Eiju Kanagawa, Takefumi Mikuriya, Makoto Hashimoto, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 35th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2012-02-25
• [Presentation] Relation Between Formation of Aggregations and Upregulation of Heart Shock Protein in Animal Models (2012)
  • Author(s): Kazuma Sugahara, Junko Tsuda, Eiju Kanagawa, Takefumi Mikuriya, Makoto Hashimoto, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 35th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2012-02-25
• [Presentation] Makoto Hashimoto, Takuo Ikeda, Kazuma Sugahara, Hironori Fujii, Hiroaki Shimogori, Hiroshi Yamashita : Analysis of the Eye Movement by Original Video-Oculography, HI-VOG (2012)
  • Author(s): Makoto Hashimoto, Takuo Ikeda, Kazuma Sugahara, Hironori Fujii, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 35th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2012-02-25
• [Presentation] Action of Substance P on the Recovery from the Acoustic Trauma (2012)
  • Author(s): Eiju Kanagawa, Kazuma Sugahara, Hideki Toyota, Takefumi Mikuriya, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 35th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2012-02-25
• [Presentation] Screening for Protective Effect in SupPlement Drugs Using the Zebrafish Lateral Line (2012)
  • Author(s): Yoshinobu Hirose, Kazuma Sugahara, Hiroshi Yamashita
  • Organizer: 35th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2012-02-25
• [Presentation] Age related hearing loss in animal model of diabetes with obesity (2011)
  • Author(s): Kazuma Sugahara, Junko Tsuda, Hiroshi Yamashita
  • Organizer: 11th Japan-Taiwan Conference on Otolaryngology-Head and Neck Surgery
  • Place of Presentation: ANAクラウンプラザ神戸神戸(講演)
  • Year and Date: 2011-12-08
• [Presentation] Intratympanic Free Radical Scavenger(Edaravone) as Rescue Therapy in Sudden Sensorineural Hearing Loss (2011)
  • Author(s): Kazuma Sugahara, Makoto Hashimoto, Takefumi Mikuriya, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 34th ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Baltimore(USA)
  • Year and Date: 2011-02-19
• [Presentation] Incase of Aggregations After Noise Injury in Guinea Pig (2011)
  • Author(s): Takefumi Mikuriya, Kazuma Sugahara, Yoshinobu Hirose, Eiju Kanagawa, Syuhei Yoshida, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 34th ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Baltimore(USA)
  • Year and Date: 2011-02-19
• [Presentation] Effect of Substance P on the Recovery from the Acoustic Trauma (2011)
  • Author(s): Eiju Kanagawa, Kazuma Sugahara, Hideki Toyota, Takefumi Mikuriya, KenjiTakeno, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 34th ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Baltimore(USA)
  • Year and Date: 2011-02-19
• [Presentation] Insulin-Like Growth Factor-1 Peptide Can Protect Vestibular Hair Cells Against the Neomycin Ototoxicity (2011)
  • Author(s): Syuhei Yoshida, Kazuma Sugahara, Eiju Kanagawa, Hideki Toyota, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 34th ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Baltimore(USA)
  • Year and Date: 2011-02-19
• [Presentation] Unilateral Intra Perilymphatic Infusion of FGLM-Amide and SSSR Facilitates Vestibular FunctionalRecovery Against AMPA-lnduced Vestibulotoxicity (2011)
  • Author(s): Hideki Toyota, Hiroaki Shimogori, Syuhei Yoshida, Kazuma Sugahara, Hirotaka Hara, Hiroshi Yamashita
  • Organizer: 34th ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Baltimore(USA)
  • Year and Date: 2011-02-19
• [Presentation] Can Antidepressant Facilitate the Recovery of Peripheral Vestibular Function from Inner Ear Damage? (2011)
  • Author(s): Hiroaki Shimogori, Hideki Toyota, Kazuma Sugahara, Makoto Hashimoto, Takefumi Mikuriya, Hiroshi Yamashita
  • Organizer: 34th ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Baltimore(USA)
  • Year and Date: 2011-02-19
• [Presentation] The Strategies against the Hair Cell Degeneration with Otoprotective Molecules (2010)
  • Author(s): Hiroshi Yamashita
  • Organizer: Sixth International Symposium on Meniere' s Disease and Inner Ear Disorders
  • Place of Presentation: 国立京都国際会館京都(シンポジスト)
  • Year and Date: 2010-11-14
• [Presentation] Effect of Unilateral Intra-perilymphatic Infusion of Substance P or P-derived Peptide(FGLM-NH2) on AMPA-induced Vestibulotoxicity in Guinea Pigs (2010)
  • Author(s): Hiroaki Shimogori, Hideki Toyota, Kenji Takeno, Hiroshi Orita, Kazuma Sugahara, Hiroshi Yamashita
  • Organizer: Sixth International Symposium on Meniere' s Disease and Inner Ear Disorders
  • Place of Presentation: 国立京都国際会館京都(講演)
  • Year and Date: 2010-11-14
• [Presentation] The Protective Effect of Resveratrol against the Hair Cell Degeneration Induced by Neomycin (2010)
  • Author(s): Kazuma Sugahara, Takefumi Mikuriya, Makoto Hashimoto, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: Sixth International Symposium onMeniere' s Disease and Inner Ear Disorders
  • Place of Presentation: 国立京都国際会館京都(講演)
  • Year and Date: 2010-11-14
• [Presentation] The Strateies against the Hair Cell Degeneration with Otoprotective Molecules (2010)
  • Author(s): Hiroshi Yamashita
  • Organizer: Sixth International Symposium on Meniere's Disease and Inner Ear Disorders
  • Place of Presentation: 京都, 京都国際会館
  • Year and Date: 2010-11-14
• [Presentation] The Role of HSFI in the Maintenance of the Inner Ear Function (2010)
  • Author(s): Hiroshi Yamashita, Kazuma Sugahara, Takefumi Mikuriya, Yoshinobu Hirose, Hiroaki Shimogori
  • Organizer: 13th Korea-Japan Joint Meeting of Otorhinolaryngology-Head and Neck Surgery 2010
  • Place of Presentation: Seoul(Korea)(講演)
  • Year and Date: 2010-09-09
• [Presentation] Unilateral intra perilymphatic infusion of sub-stance P facilitates vestibular functional recovery against AMPA-induced vestibulotoxicity (2010)
  • Author(s): Hiroshi Yamashita, Hiroaki Shimogori, Hideki Toyota, Kenji Takeno, Kazuma Sugahara, Makoto Hashimoto
  • Organizer: 26th Barany Society Meeting
  • Place of Presentation: Reykjavik(lceland)(講演)
  • Year and Date: 2010-08-18
• [Presentation] The protective effect of resveratrol on vestibular hair cells death (2010)
  • Author(s): Kazuma Sugahara, Takefumi Mikuriya, Yoshinobu Hirose, Yujirou Fukuda, Hideki Toyota, Kenji Takeno, Makoto Hashimoto, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 26th Barany Society Meeting
  • Place of Presentation: Reykjavik(lceland)(海外招待講演)
  • Year and Date: 2010-08-18
• [Presentation] Quantiative Analysis of Nystagmus by Image Analysis Technique (2010)
  • Author(s): Makoto Hashimoto, Kazuma Sugahara, Ikeda Takuo, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 33RD ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Anaheim(USA)
  • Year and Date: 2010-02-06
• [Presentation] The Expression of Proinflammatory Cytokines After Acoustic Overexposure (2010)
  • Author(s): Tetsuya Nakamoto, Takefumi Mikuriya, Kazuma Sugahara, Hiroshi Yamashita
  • Organizer: 33RD ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Anaheim(USA)
  • Year and Date: 2010-02-06
• [Presentation] The Protection of Vestibular Hair Cells with Resveratrol (2010)
  • Author(s): Kazuma Sugahara, Takefumi Mikuriya, Yoshinobu Hirose, Yujirou Fukuda, Hideki Toyota, Makoto Hashimoto, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 33RD ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Anaheim(USA)
  • Year and Date: 2010-02-06
• [Presentation] The Change of Aggregations with Heat Shock Protein 70 in the Cochlea During Aging (2010)
  • Author(s): Takefumi Mikuriya, Kazuma Sugahara, Yoshinobu Hirose, Makoto Hashimoto, Tetsuya Nakamoto, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 33RD ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Anaheim(USA)
  • Year and Date: 2010-02-06
• [Presentation] Can Inner Ear ApPlication of Rolipram, a P-CREB Up-Regurator, Induce Phosphorylation of CREB in Vestibular Ganglion Cells? (2010)
  • Author(s): Hiroaki Shimogori, Hideki Toyota, Kazuma Sugahara, Makoto Hashimoto, Takefumi Mikuriya, Yoshinobu Hirose, Hiroshi Yamashita
  • Organizer: 33RD ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
  • Place of Presentation: Anaheim(USA)
  • Year and Date: 2010-02-06
• [Presentation] The Role of HSFI in the Maintenance of the Inner Ear Function (2009)
  • Author(s): Hiroshi Yamashita, Kazuma Sugahara, Takefumi Mikuriya, Yoshinobu Hirose, Hiroaki Shimogori, Akira Nakai
  • Organizer: 10th Taiwan Japan Otolaryngology Head & Neck Conference
  • Place of Presentation: Yilan(Taiwan)(講演)
  • Year and Date: 2009-12-04
• [Presentation] HSFI is essential for the maintenance of inner ear function (2009)
  • Author(s): Hiroshi Yamashita
  • Organizer: 46th Workshop on Inner Ear Biology
  • Place of Presentation: Utrecht(Netherlands)(講演)
  • Year and Date: 2009-09-12
• [Presentation] The oral administration of tePrenone can protect the vestibular hair cells (2009)
  • Author(s): Kazuma Sugahara, Takefumi Mikuriya, Yoshinobu Hirose, Makoto Hashimoto, Hiroaki Shimogori, Hiroshi Yamashita
  • Organizer: 第19回IFOS 2009 BRAZIL
  • Place of Presentation: Sao Paulo(Brazil)
  • Year and Date: 2009-06-01
• [Presentation] Efficacy Using Animal Models (2009)
  • Author(s): Hiroshi Yamashita
  • Organizer: 第19回IFOS 2009 BRAZIL
  • Place of Presentation: Sao Paulo(Brazil)
  • Year and Date: 2009-06-01
• [Presentation] Efficacy Using Animal Models (2009)
  • Author(s): Hiroshi Yamashita
  • Organizer: 第19回IFOS 2009 BRAZIL
  • Place of Presentation: Sao Paulo, Anhembi Convention Center
  • Year and Date: 2009-06-01
• [Book] 感音難聴と酸化ストレス.よくわかる聴覚障害難聴と耳鳴のすべて (2010)
  • Author(s): 菅原一真, 山下裕司
  • Publisher: 永井書店
• [Book] 前庭神経炎の治療のEBMとは?. EBM耳鼻咽喉科・頭頸部外科の治療 (2010)
  • Author(s): 山下裕司
  • Publisher: 中外医学社
• [Book] よくわかる聴覚障害 難聴と耳鳴のすべて (2010)
  • Author(s): 菅原一真, 他
  • Publisher: 永井書店
• [Book] 再生医学-体性幹細胞と組織修復-耳鼻咽喉 (2009)
  • Author(s): 山下裕司, 菅原一真
  • Publisher: 炎症・再生医学事典
• [Book] 耳内ドラッグデリバリーと薬物療法めまいと平衡障害 (2009)
  • Author(s): 山下裕司, 下郡博明
  • Publisher: 金原出版