| Project/Area Number |
21592160
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Nozomu 九州大学, 大学病院, 助教 (60419596)
OHASHI Mitsuru 九州大学, 大学病院, 医員 (70529883)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 内耳 / 蝸牛 / 内耳有毛細胞 / カリウム電流 / 体温 / 活性化 / 不活性 / 有毛細胞 / イオンチャネル / カリウムチャネル / 温度依存性 / カルウムチャネル |
|---|
| Research Abstract |
The temperature dependence of potassium currents in isolated inner hair cells(IHCs) from mature guinea-pig cochlea was investigated. IHCs showed outwardly rectifying currents in response to depolarizing voltage pulses, with only a slight inward current when hyperpolarized. The amplitude of both outward and inward currents demonstrated no temperature dependency, however, activation and inactivation rates were faster at 36 oC than at room temperature. Half-time for activation was shorter at 36 oC than at room temperature at membrane potentials of-10,+ 10,+ 20,+ 30, and+ 40 mV. Q10 for the activation rate was 1. 83. The inactivation time constant in outward TEA-sensitive potassium currents was much smaller at 36 oC than at room temperature between the membrane potentials of-20 and+ 60 mV. Q10 for the inactivation time constant was 3. 19.
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