Promoter and inhibitor factors of bone destruction in human middle ear cholesteatoma
| Project/Area Number |
21592168
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Juntendo University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IKEDA K Atuhisa 順天堂大学, 医学部, 教授 (70159614)
HURUKAWA Masayuki 順天堂大学, 医学部, 准教授 (20359524)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | ヒト中耳真珠腫 / カテプシン / SOD / RANKL / IL-17 / 中耳真珠腫 / 骨破壊 / メタロプロテアーゼ |
|---|
| Research Abstract |
Introduction : It has been reported that cathepsin L(as one of cystein proteinases) powerfully degrade type I collagen leading to the bone destruction and receptor activator of NF-Kapper B ligand (RANKL) is an essential factor for osteoclast differentiation, furthermore, osteoclasts secrete alarge mount of cathepsin L. IL-17A can induce RANKL. Therefore, we studied cathepsin L and RANKL in human middle ear cholesteatoma. Moreover, Cu, Zn-containing superoxide dismutase(Cu, Zn-SOD) as inhibitors of cathepsin L, IL17A or RANKL were observed. From above data, It was discussed between epithelia and subepithelial granulations.
Subject and Method : The human middle ear tissues evaluated in this study were surgically obtained frompatients with cholesteatoma. Expression of specimens was Cathepsin L, RANKL and Cu, Zn-SOD were examined immunohistochemically.
Results : The degree of bone destruction was dependent on the number of IL-17 positive cells. It was a significant correlation between Il-17 and RANKL positive cells. RANKL and Il-17 immunoreactivity was detected in subepithelial granulation tissues than in epithelia. Reversely, Cu, Zn-SOD with inhibitors of RANKL and IL-17 was more strongly positive in epithelia than in subepithelial granulation tissues.
These results suggest that a field related to the bone destruction in human cholesteatoma is subepithelial granulations rather than epithelia.
|
Report
(4 results)
Research Products
(5 results)
