Investigation of microRNA expression by nasal and paranasal fibroblasts

Research Project

Project/Area Number	21592182
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Otorhinolaryngology
Research Institution	Tokyo Women's Medical University (2010-2011) Nippon Medical School (2009)
Principal Investigator	NONAKA Manabu 東京女子医科大学, 医学部, 准教授 (70271351)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	鼻副鼻腔 / microRNA / 線維芽細胞 / 呼吸器
Research Abstract	Primary fibroblast lines were established from human nasal and lung tissues. We looked at the expression of microRNA induced by cutokines. miR-146a was induced by TGF-βin lung fibroblast lines, but not in nasal fibroblast lines. IL-4/IL-13 and TNF-α induced the production of TSLP as well as miR-375 in nasal fibroblasts.