Study on thickened cell wall in clinical macrolide-resistant Staphylococcus aureus isolates.

• Project/Area Number: 21592183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kawasaki Medical School
• Principal Investigator: YAMADA Sakuo 川崎医科大学, 医学部, 准教授 (00122458)
• Co-Investigator(Kenkyū-buntansha): HARADA Tamotsu 川崎医科大学, 医学部, 教授 (30165021)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 黄色ブドウ球菌 / 細胞壁 / 肥厚 / マクロライド / ゲンタマイシン / 耐性菌 / 細胞壁肥厚 / 臨床由来耐性菌

Research Abstract

The clinical macrolide(ML)-resistant Staphylococcus aureus strains showed a thickened cell wall when compared morphologically with the clinical ML-sensitive S. aureus strains. We also demonstrated that gentamycin-resistant clinical isolates of S. aureus exhibited a thickened cell wall. These results strongly suggest that a thickened cell wall is a common ultrastractural characteristic of protein synthesis inhibitor antibiotics-resistant S. aureus clinical strains.

Research Products

• [Journal Article] Ultrastructural cell wall characteristics of clinical gentamycin-resistant Staphylococcus aureus isolates (2012)
  • Author(s): Fukutsuji Kenji, Yamada Sakuo, Harada Tamotsu
  • Journal Title: Medical Molecular Morphology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Antimicrobial effects of Burrow's solution on Staphylococcus aureus and Pseudomonas aeruginosa (2012)
  • Author(s): Hyo Yoshiyuki, Yamada Sakuo, Ishimatsu Masaki, Fukutsuji Kenji, Harada Tamotsu
  • Journal Title: Medical Molecular Morphology Volume: (in press)
  • Peer Reviewed
• [Journal Article] GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence (2012)
  • Author(s): Kawada-Matsuo M, Mazda Y, Oogai Y, Kajiya M, Kawai T, Yamada S, Miyawaki S, Oho T, Komatsuzawa H
  • Journal Title: PLos ONE Volume: (in press) Issue: 3 Pages: e33382-e33382
  • DOI: 10.1371/journal.pone.0033382
  • Peer Reviewed
• [Journal Article] 臨床由来マクロライド耐性ブドウ球菌の顕微科学的検索 (2010)
  • Author(s): 兵行義、山田作夫、原田保
  • Journal Title: 顕微鏡 Volume: 45(4) Pages: 218-222
  • Peer Reviewed
• [Journal Article] 臨床由来マクロライド耐性ブドウ球菌の顕微科学的検索 (2010)
  • Author(s): 兵行義
  • Journal Title: 顕微鏡 Volume: 45巻 Pages: 218-222
  • Peer Reviewed
• [Journal Article] Antimicrobial effects of Burrow's solution on Staphylococcus aureus and Pseudomonas aeruginosa
  • Author(s): Hyo Yukiyoshi
  • Journal Title: Medical Molecular Morphology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Ultrastructural cell wall characteristics of clinical gentamycin-resistant Staphylococcus aureus isolates
  • Author(s): Fukutsuji Kenji
  • Journal Title: Medical Molecular Morphology Volume: (in press)
  • Peer Reviewed
• [Presentation] The role in Staphylococcus aureus skin-infection, a novel cell wall protein Skip (2012)
  • Author(s): Hisatsune Junzo
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎市)
  • Year and Date: 2012-03-27
• [Presentation] GlmS and NagB modulate VicRK affecting Staphylococcus mutans virulence (2012)
  • Author(s): Kawada-Matsuo Miki
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎市)
  • Year and Date: 2012-03-27
• [Presentation] 二酸化塩素の殺芽胞作用に関する顕微科学的検索 (2012)
  • Author(s): 山田作夫
  • Organizer: 第27回日本環境感染学会総会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-02-04
• [Presentation] 次亜塩素酸ナトリウムの殺芽胞作用に関する顕微科学的解析 (2011)
  • Author(s): 山田作夫
  • Organizer: 第59回日本化学療法学会西日本支部総会
  • Place of Presentation: 奈良県新公会堂(奈良市)
  • Year and Date: 2011-11-26
• [Presentation] 次亜塩素酸ナトリウムおよび二酸化塩素の殺芽胞作用に関する顕微科学的検索 (2011)
  • Author(s): 山田作夫
  • Organizer: 日本顕微鏡学会第55回シンポジウム
  • Place of Presentation: かがわ国際会議場(高松市)
  • Year and Date: 2011-10-01
• [Presentation] Characteristic cell wall ultrastructure of a macrolide-resistant Staphylococcus capitis strain isolated from a patient with chronic sinusitis (2011)
  • Author(s): Hyo Yukiyoshi
  • Organizer: 14th International Rhinologic Society & 30th International Symposium on Infection and Allergy of the Nose
  • Place of Presentation: ANAインターコンチネンタルホテル東京(東京)
  • Year and Date: 2011-09-20
• [Presentation] ゲンタマイシンの抗黄色ブドウ球菌作用に関する超微形態的解析 (2011)
  • Author(s): 福辻賢治
  • Organizer: 第43回日本臨床分子形態学会総会学術集会
  • Place of Presentation: 大阪医科大学(高槻市)
  • Year and Date: 2011-09-09
• [Presentation] Antiseptic susceptibility and morphological changes for metallo-beta-lactamase NDM-1 producing multidrug-resistant Escherichia coli (2011)
  • Author(s): Kawai Mako
  • Organizer: IUMS2011 : International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-09-06
• [Presentation] NDM-1産生多剤耐性大腸菌の消毒薬感受性 (2011)
  • Author(s): 川井眞好
  • Organizer: 第59回日本化学療法学会総会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-06-25
• [Presentation] Possible mechanisms of cell wall thickness induced in Staphylococcus aureus by quaternary ammonium compounds of antiseptic agents (2011)
  • Author(s): Kawai Mako
  • Organizer: 111th General Meeting American Society for Microbiology
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2011-05-22
• [Presentation] Ultrastractural cell wall characteristics of clinical gentamycin-resistant Staphylococcus aureus isolates (2011)
  • Author(s): Fukutsuji Kenji, Yamada Sakuo, Harada Tamotsu
  • Organizer: 21st ECCMID : 21st European Congress of Clinical Microbiology and Infectious Diseases/27th ICC : International Congress of Chemotherapy
  • Place of Presentation: ITALIA
  • Year and Date: 2011-05-08
• [Presentation] Ultrastractural cell wall characteristics of clinical gentamycin-resistant Staphylococcus aureus isolates (2011)
  • Author(s): Fukutsuji Kenji
  • Organizer: 21st ECCMID : 21st European Congress of Clinical Microbiology and Infectious Diseases/27th ICC : International Congress of Chemotherapy
  • Place of Presentation: Milano Convention Center (Italy)
  • Year and Date: 2011-05-08
• [Presentation] 臨床由来ゲンタマイシン耐性黄色ブドウ球菌における超微形態的特徴 (2010)
  • Author(s): 福辻賢治
  • Organizer: 第42回日本臨床分子形態学会総会・学術総会
  • Place of Presentation: 東レ総合研修センター(静岡県)
  • Year and Date: 2010-09-25
• [Presentation] ゲンタマイシン処理による黄色ブドウ球菌の超微形態変化 (2010)
  • Author(s): 福辻賢治
  • Organizer: 日本耳鼻咽喉科学会第36回中国四国地方部会連合学会
  • Place of Presentation: 岡山国際交流センター(岡山県)
  • Year and Date: 2010-06-05
• [Presentation] ゲンタマイシン耐性黄色ブドウ球菌臨床分離株における細菌学的特徴 (2010)
  • Author(s): 福辻賢治、山田作夫、兵行義、原田保
  • Organizer: 第58回日本化学療法学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2010-06-03
• [Presentation] ゲンタマイシン耐性黄色ブドウ球菌臨床分離株における細菌学的特徴 (2010)
  • Author(s): 福辻賢治
  • Organizer: 第58回日本化学療法学会総会
  • Place of Presentation: 長崎ブリックホール(長崎県)
  • Year and Date: 2010-06-03
• [Presentation] ゲンタマイシン耐性黄色ブドウ球菌臨床分離株における超微形態的特徴 (2010)
  • Author(s): 福辻賢治、山田作夫
  • Organizer: 日本顕微鏡学会第66回学術講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-05-25
• [Presentation] ゲンタマイシン耐性黄色ブドウ球菌臨床分離株における超微形態的特徴 (2010)
  • Author(s): 福辻賢治
  • Organizer: 日本顕微鏡学会第66回学術講演会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2010-05-25
• [Presentation] マクロライド耐性ブドウ球菌の顕微科学的解析 (2010)
  • Author(s): 山田作夫
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-03-29
• [Presentation] マクロライド耐性ブドウ球菌の顕微科学的解析 (2010)
  • Author(s): 山田作夫
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2010-03-29
• [Presentation] ゲンタマイシン耐性黄色ブドウ球菌臨床分離株における超微形態的特徴 (2009)
  • Author(s): 山田作夫
  • Organizer: 第57回日本化学療法学会西日本支部総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2009-11-27
• [Presentation] 臨床由来ゲンタマイシン耐性黄色ブドウ球菌における特徴ある超微形態像 (2009)
  • Author(s): 山田作夫
  • Organizer: 第54回日本ブドウ球菌研究会
  • Place of Presentation: 現代医学教育博物館(倉敷市)
  • Year and Date: 2009-09-18
• [Presentation] マクロライド耐性黄色ブドウ球菌における超微形態的特徴ならびにβラクタム薬感受性 (2009)
  • Author(s): 兵行義、山田作夫、原田保
  • Organizer: 第41回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2009-09-05
• [Presentation] マクロライド耐性黄色ブドウ球菌における超微形態的特徴ならびにβラクタム感受性 (2009)
  • Author(s): 山田作夫
  • Organizer: 第41回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2009-09-05