Identification of differentially expressed microRNAs in head and neck squamous cell carcinoma based on microRNA expression signature

• Project/Area Number: 21592187
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Chiba University
• Principal Investigator: SEKI Naohiko 千葉大学, 大学院・医学研究院, 准教授 (50345013)
• Co-Investigator(Kenkyū-buntansha): HANAZAWA Toyoyuki 千葉大学, 大学院・医学研究院, 准教授 (90272327)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 癌 / マイクロRNA / 機能性核酸 / 癌抑制遺伝子 / 頭頸部扁平上皮癌 / 機能性RNA / 頭頚部扁平上皮癌

Research Abstract

MicroRNAs (miRNAs) are endogenous short non-coding RNA molecules that regulate gene expression by repressing translation or cleaving RNA transcripts in a sequence-specific manner. A growing body of evidence suggests that miRNAs are aberrantly expressed in many human cancers and that they play significant roles in the initiation, development and metastasis of human cancers. Recently, our expression profile revealed that microRNA-1, microRNA-133a and microRNA-375 are frequently downregulated in head and neck squamous cell carcinoma (HNSCC). Ectopic expression of three miRNAs (microRNA-1, microRNA-133a and microRNA-375) inhibited cell growth, migration and invasion of HNSCC cells, suggesting these microRNAs function as tumor suppressors in HNSCC.

Research Products

• [Journal Article] Tumor suppressive microRNA-133a regulates novel targets : moesin contributes to cancer cell proliferation and invasion in head and neck squamous cell carcinoma (2012)
  • Author(s): Takashi Kinoshita
  • Journal Title: Biochemical and biophysical research communications Volume: 418 Issue: 2 Pages: 378-83
  • DOI: 10.1016/j.bbrc.2012.01.030
  • Peer Reviewed
• [Journal Article] Tumour suppressive microRNA-874 regulates novel cancer networks in maxillary sinus squamous cell carcinoma (2011)
  • Author(s): Nohata N, Hanazawa T, Kikkawa N, Sakurai D, Fujimura L, Chiyomaru T, Kawakami K, Yoshino H, Enokida H, Nakagawa M, Katayama A, Harabuchi Y, Okamoto Y, Seki N
  • Journal Title: Br J Cancer Volume: 105 Pages: 833-41
  • Peer Reviewed
• [Journal Article] Tumor suppressive microRNA-375 regulates oncogene AEG-1/MTDH in head and neck squamous cell carcinoma (HNSCC) (2011)
  • Author(s): Nohata N, Hanazawa T, Kikkawa N, Mutallip M, Sakurai D, Fujimura L, Kawakami K, Chiyomaru T, Yoshino H, Enokida H, Nakagawa M, Okamoto Y, Seki N
  • Journal Title: J Hum Genet Volume: 56 Pages: 595-601
  • NAID: 40018947302
  • Peer Reviewed
• [Journal Article] Identification of novel molecular targets regulated by tumor suppressive miR-1/miR-133a in maxillary sinus squamous cell carcinoma (2011)
  • Author(s): Nohata N, Hanazawa T, Kikkawa N, Sakurai D, Sasaki K, Chiyomaru T, Kawakami K, Yoshino H, Enokida H, Nakagawa M, Okamoto Y, Seki N
  • Journal Title: Int J Oncol Volume: 39 Pages: 1099-107
  • Peer Reviewed
• [Journal Article] miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma (2011)
  • Author(s): Nohata N, Sone Y, Hanazawa T, Fuse M, Kikkawa N, Yoshino H, Chiyomaru T, Kawakami K, Enokida H, Nakagawa M, Shozu M, Okamoto Y, Seki N
  • Journal Title: Oncotarget Volume: 2 Pages: 29-42
  • Peer Reviewed
• [Journal Article] Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma (2011)
  • Author(s): Nohata N, Hanazawa T, Kikkawa N, Mutallip M, Fujimura L, Yoshino H, Kawakami K, Chiyomaru T, Enokida H, Nakagawa M, Okamoto Y, Seki N
  • Journal Title: Int J Oncol Volume: 38 Pages: 209-17
  • Peer Reviewed
• [Journal Article] Tumour suppressive microRNA-874 regulates novel cancer networks in maxillary sinus squamous cell carcinoma (2011)
  • Author(s): Nijiro Nohata
  • Journal Title: British journal of cancer Volume: 105 Issue: 6 Pages: 833-41
  • DOI: 10.1038/bjc.2011.311
  • Peer Reviewed
• [Journal Article] Identification of novel molecular targets regulated by tumor suppressive miR-1/miR-133a in maxillary sinus squamous cell carcinoma (2011)
  • Author(s): Nijiro Nohata
  • Journal Title: International journal of oncology Volume: 39 Pages: 1099-107
  • DOI: 10.3892/ijo.2011.1096
  • Peer Reviewed
• [Journal Article] Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma (2011)
  • Author(s): 野畑二次郎, 他
  • Journal Title: International Journal of Oncology Volume: 38 Pages: 209-217
  • Peer Reviewed
• [Journal Article] miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC) (2010)
  • Author(s): Kikkawa N, Hanazawa T, Fujimura L, Nohata N, Suzuki H, Chazono H, Sakurai D, Horiguchi S, Okamoto Y, Seki N
  • Journal Title: Br J Cancer Volume: 103 Pages: 877-84
  • Peer Reviewed
• [Journal Article] miR-489 is a tumor-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (2010)
  • Author(s): 吉川直子, 他
  • Journal Title: British Journal of Cancer Volume: 103 Pages: 877-884
  • Peer Reviewed
• [Journal Article] miR-145, miR-133a and miR-133b : Tumor suppressive miRNAs target FSCNI in esophageal sgamous cell carcinoma (2010)
  • Author(s): 加野将之, 他
  • Journal Title: International Journal of Cancer Volume: 127 Pages: 2804-2814
  • Peer Reviewed
• [Journal Article] Identification of novel microRNA targets based on microRNA signatrues (2009)
  • Author(s): 一美貴弘
  • Journal Title: International Journal of Cancer 125 Pages: 345-352
  • Peer Reviewed
• [Presentation] 上顎癌において腫瘍抑制機能を有するmicroRNA-1・microRNA-133aの新規分子ネットワークの解明 (2011)
  • Author(s): 野畑二次郎
  • Organizer: 第35回日本頭頸部癌学会
  • Place of Presentation: ウインクあいち(名古屋)
  • Year and Date: 2011-06-09
• [Presentation] Tumor suppressive microRNA-1/-133a regulates multiple oncogenes in maxillary sinus squamous cell carcinoma (2011)
  • Author(s): Nijiro Nohata, Toyoyuki Hanazawa, Takashi Kinoshita, Naoko Kikkawa, Muradil Mutallip, Takeshi Chiyomaru, Kazumori Kawakami, Hirofumi Yoshino, Hideki Enokida, Masayuki Nakagawa, Yoshitaka Okamoto, Naohiko Seki
  • Organizer: 16th World Congress on Advances in Oncology and 14th International Symposium on Molecular Medicine
  • Place of Presentation: Rhodes Island, Greece
• [Presentation] Tumor suppressive microRNA-375 targeting multiple oncogenes in maxillary sinus squamous cell carcinoma (MSSCC) (2011)
  • Author(s): Takashi Kinoshita, Nijiro Nohata, Toyoyuki Hanazawa, Naoko Kikkawa, Mutallip Muradil, Hirofumi Yoshino, Kazumori Kawakami, Takeshi Chiyomaru, Hideki Enokida, Masayuki Nakagawa, Yoshitaka Okamoto, Naohiko Seki
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場名古屋
• [Presentation] miR-133a as a tumor suppressive microRNA targeting multiple oncogenes in head neck squamous cell carcinoma (2011)
  • Author(s): Nijiro Nohata, Toyoyuki Hanazawa, Naoko Kikkawa, Miki Fuse, Takeshi Chiyomaru, Kazumori Kawakami, Hideki Enokida, Masayuki Nakagawa, Yoshitaka Okamoto, Naohiko Seki
  • Organizer: AACR 102nd Annual Meeting
  • Place of Presentation: Orlando, Florida
• [Presentation] Identification of tumor suppressive micro RNAs in maxillary sinus squamous cell carcinoma based on micro RNA expression signature (2011)
  • Author(s): Toyoyuki Hanazawa, Nijiro Nohata, Naoko Kikkawa, Akihiro Katayama, Yasuaki Harabuchi, Yoshitaka Okamoto, Naohiko Seki
  • Organizer: AACR 102nd Annual Meeting
  • Place of Presentation: Orlando, Florida
• [Remarks]
  • URL: http://genomejet.jp
• [Remarks]
  • URL: http://genomejet.jp