Regulation of Wnt/Snail signal and MMP onhead and neck cancer invasion and metastasis

• Project/Area Number: 21592203
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kinki University
• Principal Investigator: YANE Katsunari 近畿大学, 医学部, 教授 (40220199)
• Co-Investigator(Kenkyū-buntansha): OHNISHI Takeo 奈良医科大学, 医学部, 教授 (60094554) ; OTA Ichiro 奈良医科大学, 医学部, 助教 (00326323)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 頭頸部がん / 浸潤 / 転移 / Wnt / Snail / MMP / 浸潤・転移

Research Abstract

We demonstrate that Wnt signaling and its downstream factor, Snail, induce cancer invasion and metastasis in vivo via a matrix metalloproteinase-dependent process, using a chick chorioallantoic membrane invasion assay(CAM invasion assay). Furthermore, siRNA-specific silencing of Snail ablates the ability of cancer invasion and metastasis. Taken together, these data suggest that Snail1-targeted therapy could be effective to cancer invasion and metastasis.

Research Products

• [Journal Article] Therapeutic strategies for head and neck cancer based on p53status (2012)
  • Author(s): Ota I, Okamoto N, YaneK, Takahashi A, Masui T, Hosoi H, Onishi T
  • Journal Title: Experimental and Therapeutic Medicine Volume: 3 Pages: 585-591
• [Journal Article] Therapeutic strategies for head and neck cancer based on p53 status (2012)
  • Author(s): Ota, I.
  • Journal Title: Exp.Therap.Med. Volume: 3 Issue: 4 Pages: 585-591
  • DOI: 10.3892/etm.2012.474
  • Peer Reviewed
• [Journal Article] siRNA targeted for NBS1 enhances heat sensitivity in human anaplastic thyroid carcinoma cells (2011)
  • Author(s): Okamoto N, Takahashi A, Ota I, Ohnishi K, Mori E, Kondo N, Noda T, Nakagawa Y, Uemura H, Yane K, Hosoi H, Ohnishi T
  • Journal Title: Int J Hyperthermia Volume: 27 Pages: 297-304
  • Peer Reviewed
• [Journal Article] 頭頸部癌に対する分子標的治療の展望について (2011)
  • Author(s): 家根旦有
  • Journal Title: 耳鼻と臨床 Volume: 57 Pages: 171-176
  • NAID: 130004862286
  • Peer Reviewed
• [Journal Article] 頭頸部がんとヒトパピローマウイルス (2011)
  • Author(s): 家根旦有
  • Journal Title: 奈医報 Volume: 24 Pages: 21-25
• [Journal Article] 慢性炎症の概念と病態-発癌との関係- (2011)
  • Author(s): 家根旦有
  • Journal Title: JOHNS Volume: 27 Pages: 1769-1771
• [Journal Article] SnailによるMT1-MMPおよびMT2-MMPの癌浸潤・転移の制御 (2010)
  • Author(s): 太田一郎、家根旦有、細井裕司
  • Journal Title: 耳鼻免疫アレルギー Volume: 28(3) Pages: 235-238
• [Journal Article] Snai1によるMT1-MMPおよびMT2-MMPの癌浸潤・転移の制御 (2010)
  • Author(s): 太田一郎、家根旦有, 他
  • Journal Title: 耳鼻免疫アレルギー Volume: 28 Pages: 235-238
  • Peer Reviewed
• [Journal Article] 明口の診療に役立つ頭頸部癌の基礎研究:分子標的治療:癌幹細胞と浸潤・転移 (2010)
  • Author(s): 太田一郎、家根旦有, 他
  • Journal Title: 頭頸部癌 Volume: 36 Pages: 442-446
  • Peer Reviewed
• [Journal Article] Induction of a MT1-MMP and MT2-MMP-dependent basement membrane transmigration program in cancer cells by Snail1 (2009)
  • Author(s): Ota I, Xiao-Yan Li, Yuexian Hu, Stephen J Weiss
  • Journal Title: PNAS Volume: 106 Pages: 20318-20323
  • Peer Reviewed
• [Journal Article] Induction of a MT1-MMP and MT2-MMP-dependent basement membrane transmigration program in cancer cells by Snail1 (2009)
  • Author(s): Ichiro Ota, et al
  • Journal Title: PNAS 106 Pages: 20318-20323
  • Peer Reviewed
• [Presentation] 頭頸部癌の手術療法と化学療法 (2012)
  • Author(s): 家根旦有
  • Organizer: 第14回癌治療増感研究シンポジウム
  • Place of Presentation: 猿沢荘(奈良)
  • Year and Date: 2012-02-11
• [Presentation] siRNA targeted for NBS1 enhances heat sensitivity in human anaplastic thyroid carcinoma cells (2011)
  • Author(s): Ota I
  • Organizer: The 11th Japan-Taiwan Conference on Otolaryngology-Head and Neck Surgery
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2011-12-08
• [Presentation] siRNA targeted for NBS1 enhances heat sensitivity in human anaplastic thyroid carcinoma cells (2011)
  • Author(s): Okamoto N, et al
  • Organizer: The 11th Japan-Taiwan Conference on Otolaryngology-Head and Neck Surgery
  • Place of Presentation: ANA CROWNE PLAZA HOTEL (Kobe)
  • Year and Date: 2011-12-08
• [Presentation] 頭頸部癌細胞におけるEGFRおよびmTORシグナル経路をターゲットにした分子標的治療 (2011)
  • Author(s): 太田一郎, 他
  • Organizer: 第35回日本頭頸部癌学会
  • Place of Presentation: ウインクあいち(名古屋)
  • Year and Date: 2011-06-09
• [Presentation] 頭頸部癌に対する分子標的治療の展望について (2011)
  • Author(s): 家根旦有
  • Organizer: 第16回頭頸部癌化学療法研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-02-03
• [Presentation] 頭頸部癌治療とHPV感染 (2010)
  • Author(s): 家根旦有
  • Organizer: 第3回喉頭機能温存治療研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-18
• [Presentation] Snail mobilizes the membrane-anchored collagenases, MT1-MMP and MT2-MMP, to drive cancer cell invasion programs (2010)
  • Author(s): Ota I
  • Organizer: The 13th Japan-Korea Joint Meeting of Otolaryngology-Head and Neck Surgery
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-09-11
• [Presentation] Snail mobilizes the membrane-anchored collagenases, MT1-MMP and MT2-MMP, to drive cancer cell invasion programs (2010)
  • Author(s): Ichiro Ota, et al
  • Organizer: The 13th Korea-Japan Joint Meeting of Otorhinolaryngology-Head and Neck Surgery
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-09-11
• [Presentation] SnailによるMT1-MMPおよびMT2-MMPの癌浸潤・転移能の制御 (2010)
  • Author(s): 家根旦有
  • Organizer: 第16回国際癌治療増感研究会
  • Place of Presentation: 岐阜
  • Year and Date: 2010-06-19
• [Presentation] SnallによるMT1-MMPおよびMT2-MMPの癌浸潤・転移能の制御 (2010)
  • Author(s): 太田一郎, 他
  • Organizer: 第16回国際癌治療増感研究会
  • Place of Presentation: 岐阜市
  • Year and Date: 2010-06-19
• [Presentation] SnailによるMT1-MMPおよびMT2-MMP依存性の癌浸潤能の誘導 (2010)
  • Author(s): 太田一郎
  • Organizer: 第111回日本耳鼻咽喉科総会
  • Place of Presentation: 仙台
  • Year and Date: 2010-05-19
• [Presentation] SnailによるMT1-MMPおよびMT2-MMP依存性の癌浸潤能の誘導 (2010)
  • Author(s): 太田一郎, 他
  • Organizer: 第111回日本耳鼻咽喉科総会学術集会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-05-19
• [Presentation] Snail mobilizes the membrane-anchored collagenases MT1-MMP and MT2-MMP to drive cancer cell invasion programs in vivo (2010)
  • Author(s): Ota I
  • Organizer: 100th Annual Meeting 2009
  • Place of Presentation: Denver, USA
  • Year and Date: 2010-04-11
• [Presentation] Snail1によるMT-MMPおよびMT2-MMPの癌浸潤・転移能の制御 (2010)
  • Author(s): 太田一郎
  • Organizer: 第28回耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 福井
  • Year and Date: 2010-02-19
• [Presentation] Snail1 によるMT-MMPおよびMT2-MMPの癌浸潤・転移能の制御 (2010)
  • Author(s): 太田一郎, 家根旦有, 他
  • Organizer: 第28回耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: AOSSA(福井)
  • Year and Date: 2010-02-19
• [Presentation] Snailを介したWntシグナル伝達によるがんの浸潤・転移の制御 (2010)
  • Author(s): 家根旦有
  • Organizer: 第12回癌治療増感研究シンポジウム
  • Place of Presentation: 奈良
  • Year and Date: 2010-02-14
• [Presentation] Snail を介した Wnt シグナル伝達によるがんの浸潤・転移の制御 (2010)
  • Author(s): 太田一郎, 家根旦有, 他
  • Organizer: 第12回癌治療増感研究シンポジウム
  • Place of Presentation: 猿沢荘(奈良)
  • Year and Date: 2010-02-14
• [Presentation] Snail1 induces the membrane-anchored collagenases, MT1-MMP and MT2-MMP, to drive cancer cell invasion programs in vivo (2009)
  • Author(s): Ota I
  • Organizer: 第68回日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] Snail1 induces the membrane-anchored collagenases, MT1-MMP and MT2-MMP, to drive cancer cell invasion programs in vivo (2009)
  • Author(s): Ichiro Ota, Katsunari Yane, et al
  • Organizer: 第68回日本癌学会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-10-02
• [Presentation] The membrane-anchored collagenase MT1-MMP and MT2-MMP mediate tumor cell traffic through the extracellular matrix cooperatively in vivo (2009)
  • Author(s): Ichiro Ota, Katsunari Yane, et al
  • Organizer: American Association of Cancer Research
  • Place of Presentation: Colorado Convention Center(Colorado)
  • Year and Date: 2009-04-22