| Project/Area Number |
21592204
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
|
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
TAMURA Shinji 和歌山県立医科大学, 医学部, 講師 (10244724)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMANAKA Noboru 和歌山県立医科大学, 医学部, 教授 (10136963)
HOTOMI Muneki 和歌山県立医科大学, 医学部, 講師 (90336892)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | MALファミリー / 頭頸部癌 / 転移 / メチル化 / 転移関連遺伝子 / MAL遺伝子 / 早期診断 |
|---|
| Research Abstract |
MAL(Myelin and Lymphocyte protein) family of preoteolipids including MAL, MAL2 and BENE are implicated to be potent regulator of apical protein transport and cell polarization in epithelial cells. In this study, we evaluated expression of MAL2 and BENE genes in matched primary and metastatic tumors of head and neck squamous cell carcinoma(HNSCC). We also analyzed loss of heterogeneity(LOH) status of MAL2 and BENE gene. As a result, down-regulation of MAL mRNA expression was remarkable in metastatic HNSCC tumors and it was significantly related with LOH in metastatic HNSCC. Furthermore, we evaluated role of circulating hypermethylated MAL gene as a diagnostic marker in HNSCC. We found hypermethylation of MAL promoter in five out of eight(62%) plasma samples from HNSCC patients. Therefore, detection of hypermethylation of MAL promoter in plasma may be a promising diagnostic marker for HNSCC.
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