the study of internalization of Haemophilus influenzae and Streptococcus pyogenes into epithelial cells in upper respiratory infectious disease.

• Project/Area Number: 21592205
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Wakayama Medical University
• Principal Investigator: TOGAWA Akihisa 和歌山県立医科大学, 医学部, 講師 (70305762)
• Co-Investigator(Kenkyū-buntansha): 藤原 啓次 和歌山県立医科大学, 医学部, 准教授 (20244725) ; 島田 純 和歌山県立医科大学, 医学部, 講師 (90326372)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 咽頭科学 / 溶連菌 / 急性扁桃炎 / 急性鼻副鼻腔炎 / インフルエンザ菌 / 細胞内侵入 / 急性副鼻腔炎 / 急性中耳炎

Research Abstract

Streptoococcus pyogenes and nontypeable Haemphyrus influenzae are important causative pathogens of upper respiratory infectious disease. To examine the internalization of these pathogens into human epithelial cells, we investigate the gene that may cause the internalization. We detected 12. 0% of sil gene in non-invasive infection and 17. 7% in invasive infection.

Research Products

• [Journal Article] Evaluation of a Rapid Immunochromatographic ODK-0901 Test for Detection of Pneumococcal Antigen in Middle Ear Fluids and Nasopharyngeal Secretions (2012)
  • Author(s): Hotomi M, Togawa A, 他13名
  • Journal Title: Plos One Volume: (7) 3
  • Peer Reviewed
• [Journal Article] Evaluation of a Rapid Immunochromatographic ODK-0901 Test for Detection of Pneumococcal Antigen in Middle Ear Fluid and Nasopharyngeal Secretons (2012)
  • Author(s): Muneki Hotomi
  • Journal Title: PLoS One Volume: 7(3) Pages: 1-7
  • Peer Reviewed
• [Journal Article] Clinical scoring system of Acute Pharyngotonsillitis (2011)
  • Author(s): Togawa A, Hotomi M, Tamura S, Yamanaka N
  • Journal Title: Adv Otolaryngol Volume: 72 Pages: 139-41
• [Journal Article] 成人急性鼻副鼻腔炎のスコアリングシステムによる抗菌薬の有効性評価に関する検討 (2011)
  • Author(s): 山中昇
  • Journal Title: 耳鼻咽喉科臨床 Volume: 104.10 Pages: 741-754
  • Peer Reviewed
• [Journal Article] A comparison of conventional and molecular microbiology in detectiong differences in pneumococcal colonization in healthy children and children with upper respiratory illness (2010)
  • Author(s): Ogami M, Hotomi M, Togawa A, Yamanaka N
  • Journal Title: Eur J Pediatr Volume: 169(10) Pages: 1221-5
  • Peer Reviewed
• [Journal Article] 成人急性咽頭・扁桃炎に対するガレノキサシン(GRNX)の有用性に関する検討-スコアリングシステムを用いた重症度評価とその臨床効果- (2010)
  • Author(s): 山中昇
  • Journal Title: 耳展 Volume: 53 Pages: 142-152
  • Peer Reviewed
• [Journal Article] Haemophilus influenzae and Haemophilus haemolyticus in tonsillar cultures of adults with acute pharyngotonsillitis (2010)
  • Author(s): Hotomi M, Kono M, Togawa A, 他6名
  • Journal Title: Auris Nasus Larynx Volume: 37 Pages: 594-600
  • Peer Reviewed
• [Journal Article] 急性中耳炎に対するTebipenem pivoxilの有効性スコアリング・システムを用いた重症度評価とその臨床効果 (2010)
  • Author(s): 山中昇、保富宗城、戸川彰久、竹井慎、河野正充
  • Journal Title: 耳鼻咽喉科臨床 Volume: 103(1) Pages: 77-84
  • Peer Reviewed
• [Journal Article] 成人急性咽頭・扁桃炎に対するガレノキサシン(GRNX)の有用性に関する検討-スコアリングシステムを用いた重症度評価とその臨床効果-山中昇 (2010)
  • Author(s): 保富宗城、戸川彰久、池田頼彦、田村真司
  • Journal Title: 耳展 Volume: 53 Pages: 142-152
  • Peer Reviewed
• [Journal Article] Nontypeable Haemophilus influenzae isolated from intractable acute otitis media internalized into cultured human epithelial cells (2009)
  • Author(s): Hotomi M
  • Journal Title: Auris Nasus Larynx. Jun 7. Pages: 137-44
  • Peer Reviewed
• [Journal Article] Distribution of fibronectin-binding protein genes(prtF1 and prtF2)and streptococcal pyrogenic exotoxin genes(spe)among Streptococcus pyogenes in Japan (2009)
  • Author(s): Hotomi M
  • Journal Title: J Infect Chemother. Dec;15(6) Pages: 367-73
  • Peer Reviewed
• [Presentation] 小児急性中耳炎に対する新規抗菌薬TBPM-PIとTFLXの有効性の検討 (2012)
  • Author(s): 戸川彰久, 兼定啓子, 末武光, 他7名
  • Organizer: 第113回日本耳鼻咽喉科学会総会
  • Place of Presentation: 新潟
• [Presentation] 成人急性鼻副鼻腔炎に対するLVFX 500mg 1日1回投与の有用性に関する検討 (2011)
  • Author(s): 戸川彰久、山中昇、杉田麟也, 他7名
  • Organizer: 第73回耳鼻咽喉科臨床学会
  • Place of Presentation: 松本
• [Presentation] Delayed Antimicrobial Treatment Does Not Change the Clinical Course of Non-Severe Acute Otitis Media (2011)
  • Author(s): Togawa A, Hotomi M, Hiraoka M, 他6名
  • Organizer: 10th International Symposium of Recent Advances in Otitis Media
  • Place of Presentation: New Orleans, USA
• [Presentation] Clinical Efficacy of Middle Ear Ventilation Tube Insertion Against Intractable Acute Otitis Media (2011)
  • Author(s): Togawa A, Hotomi M, Takei S, 他5名
  • Organizer: 10th International Symposium of Recent Advances in Otitis Media
  • Place of Presentation: New Orleans, USA
• [Presentation] Clinical Efficacy of Middle Ear Ventilation Tube Insertion Against Intractable Acute Otitis Media (2011)
  • Author(s): Akihisa Togawa
  • Organizer: 10^<th> International Symposium of Recent Advances in Otitis Media
  • Place of Presentation: New Orleans, USA
• [Presentation] Treatment of pharyngo-tonsillitis : clinical scoring system of acute pharyngitis and acute tonsillitis Togawa (2010)
  • Author(s): A, Hotomi M, Takei S, Yamanaka N
  • Organizer: 7th International Symposium on Tonsils and Mucosal Barriers of the Upper Airways
  • Place of Presentation: Asahikawa, Japan
• [Presentation] Efficacy of ventilation tube insertion against pediatric intractable otitis media (2009)
  • Author(s): Akihisa Togawa
  • Organizer: 6^<th> extraordinary International Symposium on Recent Advances in Otitis Media
  • Place of Presentation: Seoul, Korea