Pathogenesis of abnormal fundus autofluorescence and its relevance to age-related macular degeneration

• Project/Area Number: 21592236
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nagoya City University
• Principal Investigator: YASUKAWA Tsutomu 名古屋市立大学, 大学院・医学研究科, 准教授 (00324632)
• Co-Investigator(Kenkyū-buntansha): OGURA Yuichiro 名古屋市立大学, 大学院・医学研究科, 教授 (70191963) ; NOZAKI Miho 名古屋市立大学, 大学院・医学研究科, 講師 (00295601)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 眼細胞生物学 / 加齢黄斑変性 / 家兎 / 眼底異常自発蛍光 / 網膜色素上皮 / リポフスチン / 家兔

Research Abstract

Age-related macular degeneration(AMD) is a major cause of legal blindness in the adult. Understanding of aging changes in eyes may be required to elucidate the pathogenesis of AMD and develop new prophylactic modalities. The first senescent change in the eye is the accumulation of lipofuscin, granules refractory to lysosomal degradation into retinal pigment epithelial cells. In this study, the rabbit AMD model was developed by injecting imitation lipofuscin into the subretinal space. This model revealed abnormal fundus autofluorescence similar to the findings observed in human aging eyes with or without AMD. This model mimicked the pathology of AMD and might be useful to elucidate the biogenesis of abnormal fundus autofluorescence and the pathogenesis of AMD.

Research Products

• [Journal Article] Recent advances in intraocular drug delivery systems (2011)
  • Author(s): Yasukawa T, Tabata Y, Kimura H, Ogura Y
  • Journal Title: Recent Pat Drug Deliv Formul Volume: 5 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Ocular drug delivery for bioactive protein (2011)
  • Author(s): Yasukawa T, Tabata Y, Kimura H, Ogura Y
  • Journal Title: Expert Rev Ophthalmol Volume: 6 Pages: 657-667
  • Peer Reviewed
• [Journal Article] Pneumatic displacement of submacular hemorrhage with or without tissue plasminogen activator (2011)
  • Author(s): Mizutani T, Yasukawa T, Ito Y, Takase A, Hirano Y, Yoshida M, Ogura Y
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 249 Pages: 1153-1157
  • Peer Reviewed
• [Journal Article] Anti-angiogenic effects of the receptor tyrosine kinase inhibitor, pazopanib, on choroidal neovascularization in rats (2011)
  • Author(s): Yafai Y, Yang XM, Niemeyer M, Nishiwaki A, Lange J, Wiedemann P, King AG, Yasukawa T, Eichler W
  • Journal Title: Eur J Pharmacol Volume: 666 Pages: 12-18
  • Peer Reviewed
• [Journal Article] Recent advances in intraocular drug delivery systems. (2011)
  • Author(s): Yasukawa T, et al.
  • Journal Title: Recent Pat Drug Deliv Formul. Volume: 5 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Medical devices for the treatment of eye diseases (2010)
  • Author(s): Yasukawa T, Ogura Y
  • Journal Title: Handb Exp Pharmacol Volume: 197 Pages: 469-489
  • Peer Reviewed
• [Journal Article] Indocyanine green angiography-guided laser photocoagulation combined with sub-tenon's capsule injection of triamcinolone acetonide for idiopathic macular telangiectasia (2010)
  • Author(s): Hirano Y, Yasukawa T, Usui Y, Nozaki M, Ogura Y
  • Journal Title: Br J Ophthalmol Volume: 94 Pages: 600-605
  • Peer Reviewed
• [Journal Article] Inflammation in age-related macular degeneration : pathological or physiological? Editorial (2009)
  • Author(s): Yasukawa T
  • Journal Title: Expert Rev Ophthalmol Volume: 4 Pages: 107-112
  • Peer Reviewed
• [Journal Article] Fundus autofluorescence and fate of glycoxidized particles injected into subretinal space in rabbit age-related macular degeneration model (2009)
  • Author(s): Hirata M, Yasukawa T, Wiedemann P, Kimura E, Kunou N, Eichler W, Takase A, Sato R, Ogura Y
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 247 Pages: 929-937
  • Peer Reviewed
• [Journal Article] Intraocular pressure elevation following triamcinolone acetonide administration as related to administration routes (2009)
  • Author(s): Hirano Y, Ito T, Nozaki M, Yasukawa T, Sakurai E, Yoshida M, Ogura Y
  • Journal Title: Jpn J Ophthalmol Volume: 53 Pages: 519-522
  • NAID: 10025865218
  • Peer Reviewed
• [Journal Article] Fundus autofluorescence and fate of glycoxidized particles injected into subretinal space in rabbit age-related macular degeneration model (2009)
  • Author(s): Hirata M, Yasukawa T, et al.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol 247 Pages: 929-937
  • Peer Reviewed
• [Presentation] 加齢黄斑変性初期病態に関する臨床研究報告(2)-眼底異常所見と網膜感度の関連- (2010)
  • Author(s): 安川力
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 神旨ポートピアホテル(兵庫)
  • Year and Date: 2010-11-11
• [Presentation] JFAMスタディグループ:加齢黄斑変性初期病態に関する臨床研究報告(2)-眼底異常所見と網膜感度の関連-(加齢黄斑変性初期病態調査(2)) (2010)
  • Author(s): 安川力
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 神戸
• [Presentation] In vitro drusen model : 3D-spheroid culture of retinal pigment epithelial cells (2010)
  • Author(s): Yasukawa T, Wiedemann P, Ogura Y
  • Organizer: 27^<th> meeting of the Club Jules Gonin
  • Place of Presentation: Kyoto, Japan
• [Presentation] Drug delivery systems for treatment of macular diseases. Symposium. Advances in molecular biology of macular diseases (2010)
  • Author(s): Yasukawa T
  • Organizer: The 25^<th> Congress of APAO
  • Place of Presentation: Beijing, China
• [Presentation] 異常眼底自発蛍光の発生機序と加齢黄斑変性との関連 (2009)
  • Author(s): 安川力
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2009-04-18
• [Presentation] Multicellular spheroid culture of retinal pigment epithelial cells enhancing differentiation as an epithelium and formation of Bruch's membrane (2009)
  • Author(s): Yasukawa T, Wiedemann P, Wolfram E, Yafai Y, Nishiwaki A, Bringmann A, Ogura Y
  • Organizer: 107th DOG Congress
  • Place of Presentation: Leipzig
• [Presentation] Three-dimensional spheroid culture visualizing membranogenesis of Bruch's membrane and basal functions of retinal pigment epithelium (2009)
  • Author(s): Yasukawa T, Wiedemann P, Nishiwaki A, Takase A, Sato R, Ogura Y
  • Organizer: The 24^<th> Congress of APAO
  • Place of Presentation: Bali, Indonesia