Development of the new treatment strategy through the TRP channel to severe neurotrophic keratopathy and alkali burn in cornea

• Project/Area Number: 21592241
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Wakayama Medical University
• Principal Investigator: OKADA Yuka 和歌山県立医科大学, 医学部, 講師 (50264891)
• Co-Investigator(Kenkyū-buntansha): SAIKA Shizuya 和歌山県立医科大学, 医学部, 教授 (40254544)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 角膜 / 創傷治癒 / TRPチャンネル / TRP / 炎症 / 三叉神経

Research Abstract

Absence or blocking TRP channels suppressed inflammation and fibrosis/sacrring during healing of alkali-burnec cornea. Systemic TRP channel antagonists reproduced the KO-type of healing. In conclusion, absence or blocking of TRPV1 suppressed inflammation and fibrosis/scarring during healing of severe neurotrophic keratopaty and alkali burn in cornea.

Research Products

• [Journal Article] TRPV1 involvement in inflammatory tissue fibrosis in mice (2011)
  • Author(s): Okada Y, Reinach PS, Shirai K, Kitano A, Kao WW, Flanders KC, Miyajima M, Liu H, Zhang J, Saika S
  • Journal Title: Am J Pathol Volume: 178(6) Pages: 2654-64
• [Journal Article] 【ケアの違いはここにある!糖尿病VS非糖尿病】ケアする 症状と合併症 ケアの違いはここにある 視力障害(解説/特集) (2010)
  • Author(s): 岡田由香
  • Journal Title: 透析ケア Volume: 16(7) Pages: 742-745
• [Journal Article] Therapeutic potential of trichostatin A to control inflammatory and fibrogenic disorders of the ocular surface. (2010)
  • Author(s): Kitano A, Okada Y, Yamanka O, Shirai K, Mohan RR, Saika S
  • Journal Title: Mol Vis. Volume: 31;16 Pages: 2964-73
  • Peer Reviewed
• [Journal Article] Impaired angiogenic response in the cornea of mice lacking tenascin C. (2010)
  • Author(s): Sumioka T, Fujita N, Kitano A, Okada Y, Saika S.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 18(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Suppression of injury-induced epithelial-mesenchymal transition in a mouse lens epithelium lacking tenascin-C. (2010)
  • Author(s): Tanaka S, Sumioka T, Fujita N, Kitano A, Okada Y, Yamanaka O, Flanders KC, Miyajima M, Saika S.
  • Journal Title: Mol Vis Volume: 1;16 Pages: 1194-205
  • Peer Reviewed
• [Journal Article] Two Cases of Congenital Retrobulbar Cyst or Peripapillary Staphyloma with an Alcoholic Mother. (2010)
  • Author(s): Kitano A, Shirai K, Okada Y, Saika S.
  • Journal Title: J Pediatr Ophthalmol Strabismus. Volume: 26 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Immunohistochemical observation of anterior subcapsular cataract in eye with spontaneously regressed retinoblastoma (2010)
  • Author(s): Shirai K, Okada Y, Saika S.
  • Journal Title: J Cataract Refract Surg. Volume: 36(3) Pages: 503-7
  • Peer Reviewed
• [Journal Article] Impaired angiogenic response in the corneas of mice lacking osteopontin. (2010)
  • Author(s): Fujito N, Fujito S, Okada Y, Fujita K, Kitano A, Yamanaka O, Miyamoto T, Kon S, Uede T
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 51(2) Pages: 790-4
  • Peer Reviewed
• [Journal Article] 免疫再構築症候群の一例 (2010)
  • Author(s): 洪成勲, 岡田由香, 雑賀司珠也
  • Journal Title: 眼科臨床紀要 Volume: 3(8) Pages: 768-770
  • NAID: 10030250889
  • Peer Reviewed
• [Journal Article] Neurotrophic keratopathy ; its pathophysiology and treatment. (2010)
  • Author(s): Okada Y, Reinach PS, Kitano A, Shirai K, Kao WW, Saika S.
  • Journal Title: Histol Histopathol. Volume: 25(6) Pages: 771-80
  • Peer Reviewed
• [Journal Article] Transforming growth factor beta signal transduction : a potential target for maintenance/restoration of transparency of the cornea. (2010)
  • Author(s): Saika S, Yamanaka O, Sumioka T, Okada Y, Miyamoto T, Shirai K, Kitano A, Tanaka S.
  • Journal Title: Eye Contact Lens. Volume: 36(5) Pages: 286-9
  • Peer Reviewed
• [Presentation] マウス三叉神経障害モデルの角膜創傷治癒 (2012)
  • Author(s): 岡田由香、白井久美、藤田識人, 泉谷愛、仙波恵美子、雑賀司珠也
  • Organizer: 角膜カンファランス2012
  • Place of Presentation: 東京
  • Year and Date: 2012-02-23
• [Presentation] TRP receptors and corneal inflammation (2012)
  • Author(s): Okada Y, Reinach P, Shirai K, Kitano A. Saika S
  • Organizer: Gordon Reserch Conferences
  • Place of Presentation: Ventura. USA
• [Presentation] マウス三叉神経障害モデルの作製 (2011)
  • Author(s): 岡田由香、仙波恵美子、白井久美、藤田識人、北野愛、雑賀司珠也
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-12
• [Presentation] Effects of loss of TRPV4 on the inflammation and scarring of analkali-burned cornea in mice (2011)
  • Author(s): Yuka Okada, Peter S, Reinach, Kumi Shirai, Ai Kitano, Masayasu Miyajima, Shizuya Saika
  • Organizer: ARVO
  • Place of Presentation: フロリダ
  • Year and Date: 2011-05-02
• [Presentation] Effects of loss of TRPV4 on the inflammation and scarring of an alkali-burned cornea in mice (2011)
  • Author(s): Okada Y, Reinach P, Shirai S, Kitano A, Miyajima M, Saika S
  • Organizer: ARVO
  • Place of Presentation: Ft. lauderdale USA
• [Presentation] Thermosensitive ion channel receptors and corneal inflammation (2010)
  • Author(s): Yuka Okada, Peter Reinach, Kumi Shirai, Ai Kitano, Masayasu Miyajima, Winston W.-Y.Kao, Shizuya Saika
  • Organizer: 日韓ジョイント角膜カンファランス
  • Place of Presentation: 京都
  • Year and Date: 2010-12-04
• [Presentation] Modulation of inflammation in corneal etroma by ion channel signaling (2010)
  • Author(s): Yuka Okada, Peter Reinach, Kumi Shirai, Ai Kitano, Masayasu Miyajima, Winston W.-Y.Kao, Shizuya Saika
  • Organizer: International Congress of Eye Research
  • Place of Presentation: モントリオール
  • Year and Date: 2010-07-19
• [Presentation] TRPA1 gene ablation in mice improves corneal wound healing response to injury (2010)
  • Author(s): Yuka Okada, Peter Reinach, Kumi Sirai, Ai Kitano, Masayasu Miyajima, Shizuya saika
  • Organizer: The Association for Research in Vision and Ophthalmology
  • Place of Presentation: フォートローダーデール
  • Year and Date: 2010-05-04
• [Presentation] TRPV4欠損の角膜アルカリ外傷に及ぼす影響 (2010)
  • Author(s): 岡田由香, ピーター・ライナック, 白井久美, 北野愛, 宮嶋正康, 雑賀司珠也
  • Organizer: 日本眼科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-15
• [Presentation] TRPA1欠損の角膜アルカリ外傷に及ぼす影響 (2010)
  • Author(s): 岡田由香, ピーターライナック, 白井久美, 北野愛, 宮嶋正康, 雑賀真珠也
  • Organizer: 角膜カンファランス
  • Place of Presentation: 仙台
  • Year and Date: 2010-02-11
• [Presentation] Thermosensitive ion channel receptors and corneal inflammation (2010)
  • Author(s): Okada Y, Reinach P, Shirai K, Kitani A, Miyajima M, Kao WW, Saika S
  • Organizer: 2010 Korea-Japan Joint Cornea Conference
  • Place of Presentation: Kyoto
• [Presentation] TRPV4 gene ablation in mice improves corneal wound healing response to injury (2010)
  • Author(s): Okada Y, Reinach P, Shirai K, Kitani A, Miyajima M, Kao WW, Saika S
  • Organizer: The 2^<nd> Asia Cornea Society Biennial Scientific Meeting
  • Place of Presentation: Kyoto
• [Presentation] Modulation of inflammation in corneal stroma by ion channel signaling (2010)
  • Author(s): Okada Y, Reonach P, Shirai K, Kitano A, Miyajima M, Kao WW, Saika S
  • Organizer: ICER
  • Place of Presentation: Montreal Canada
• [Presentation] TRPA1 gene ablation in mice improves corneal wound healing response to injury (2010)
  • Author(s): Okada Y, Reinach P, Shirai K, Kitano A, Miyajima M, Saika S
  • Organizer: ARVO
  • Place of Presentation: Ft. lauderdale USA
• [Presentation] Loss of mouse TRPV1 function improves corneal wound healing response to alkali-burn injury (2010)
  • Author(s): Okada Y, Saika S, Shirai, K, Kitano A, Miyajima M, Kao WW, Reinach P
  • Organizer: Gordon Reserch Conferences
  • Place of Presentation: Ventura. USA
• [Presentation] Effects of loss of TRPV1 on the inflammation after an alkali-burned cornea in mice (2009)
  • Author(s): Yuka Okada, Shizuya Saika, Kumi Shirai, Ai Kitano, Masayasu Miyajima, Peter Reinach
  • Organizer: ARVO
  • Place of Presentation: フロリダ
  • Year and Date: 2009-05-03
• [Presentation] TRPV1受容体阻害薬のマウス角膜アルカリ外傷後の治癒への効果 (2009)
  • Author(s): 岡田由香, ピーターライナック北野愛, 白井久美, 宮嶋正康, 雑賀真珠也
  • Organizer: 日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-16
• [Presentation] Effects of loss of TRPV1 on the inflammation after an alkali-burned cornea in mice (2009)
  • Author(s): Okada Y, Saika S, Shirai K, KJitano A, Miyajima M, Reinach P
  • Organizer: ARVO
  • Place of Presentation: Ft. lauderdale USA