| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Retinal neuroprotective effect of PACAP was evaluated in transient ischemia model induced by high intraocular pressure. PACAP suppressed retinal ganglion cells(RGCs) with bimodal fashion with cAMP and MAPK pathways. Retinal protective effect of endogenous PACAP was examined using heterozygous PACAP KO mice with NMDA model. heterozygous PACAP KO mice showed high number of RGCs and less number of TUNEL positive cells in NMDA-treated retina. These data suggest that exogenous and endogenous PACAP have a neuroprotective potential in retinal RGCs. Furthermore, number of BrdU positive cells peaked on day 3 after NMDA treatment, and about 90% of BrdU positive cells were overlapped with microglia/macrophage marker. PACAP administration increased the number of BrdU positive cells after NMDA-treatment. Increment of the number of BrdU positive cells may be involved in neuroprotective effect of PACAP.
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