Pharmaokinetics of intravitreally injected vascular endothelial growth factor

• Project/Area Number: 21592255
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: OHJI Masahito 滋賀医科大学, 医学部, 教授 (90252650)
• Co-Investigator(Kenkyū-buntansha): KAWAMURA Hajime 滋賀医科大学, 医学部, 講師 (50294814) ; SAWADA Osamu 滋賀医科大学, 医学部, 助教 (00378465)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: VEGF / VEGF阻害薬 / 薬物動態 / 硝子体注射 / サル / bevacizumab / VEGF阻害剤 / 約粒動態

Research Abstract

We evaluated the pharmacokinetics of intravitreally injected bevacizumab which is widely used as an off-label drug. Intravitreal injection of regular dose of bevacizumab(1. 25mg) inhibited VEGF for 4-6 weeks. Minimum effective dose of bevacizumab to suppress VEGF was 6. 25μg which is 1/200 of the regular dose. The half-life of intravitreally injected bevacizumab was 1. 5 days in a-vitreous eyes while it was 2. 8 days in normal eyes. These findings would be useful information for the safe and effective use of bevacizumab.

Research Products

• [Journal Article] Negative Correlation between Aqueous Vascular Endothelial Growth Factor Levels and Axial Length (2011)
  • Author(s): Sawada O, Miyake T, Kakinoki M, Sawada T, Kawamura H, Ohji M
  • Journal Title: Jpn J Ophthalmol Volume: 55 Pages: 401-404
  • NAID: 10029528239
  • Peer Reviewed
• [Journal Article] Vascular Endothelial Growth Factor in the aqueous humor in eyes with myopic choroidal neovascularization (2011)
  • Author(s): Sawada o, Kawamura H, Kakinoki M, Sawada T, Ohji M
  • Journal Title: Acta Ophthalmol Volume: 89 Issue: 5 Pages: 459-62
  • DOI: 10.1111/j.1755-3768.2009.01717.x
  • Peer Reviewed
• [Journal Article] Pharmacokinetics of bevacizumab and its effect on vascular endothelial growth factor after intravitreal injection of bevacizumab in Macaque eyes (2010)
  • Author(s): Miyake T, Sawada O, Kakinoki M, Sawada T, Kawamura H, Ogasawara K, Ohji M.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51巻 Pages: 1606-1608
  • Peer Reviewed
• [Journal Article] Vascular Endothelial Growth Factor in the aqueous humor in eyes with myopic choroidal neovascularization. (2010)
  • Author(s): Sawada O, Kawamura H, Kakinoki M, Sawada T, Ohji M
  • Journal Title: Acta Ophthalmol Volume: Jan 22 E-Pub ahead of print
  • Peer Reviewed
• [Journal Article] Aqueous Vascular Endothelial Growth Factor after Intravitreal Injection of Pegaptanib or Ranibizumab in Patients with Age-Related Macular Degeneration. (2010)
  • Author(s): Sawada O, Miyake T, Kakinoki M, Sawada T, Kawamura H. Ohji M
  • Journal Title: Retina Volume: 30 Pages: 1034-1038
  • Peer Reviewed
• [Journal Article] Pharmacokinetics of bevacizumab and its effect on vascular endothelial growth factor after intravitreal injection of bevacizumab in Macaque eyes (2010)
  • Author(s): Miyake T, Sawada O, Kakinoki M, Sawada T, Kawamura H, Ogasawara K, Ohji M
  • Journal Title: Investigative Ophthalmology and Visual Science 51 Pages: 1606-1608
  • Peer Reviewed
• [Presentation] Pharmacokinetics of ranibizumab and its effect on vascular endothelial growth factor after intravitreal injection of ranibizumab in macaque eyes (2011)
  • Author(s): Miyake T, Kakinoki M, Sawada O, Sawada T, Saishin Y, Kawamura H, Ohji M.
  • Organizer: 第50回日本網膜硝子体学会
  • Place of Presentation: 東京
  • Year and Date: 2011-12-03
• [Presentation] カニクイザルの水晶体嚢温存無硝子体眼へのべバシズマブ投与におけるVEGF濃度の解析 (2011)
  • Author(s): 柿木雅志、三宅太一郎、澤田智子、西信良嗣、川村肇、大路正人
  • Organizer: 第50回日本網膜硝子体学会
  • Place of Presentation: 東京
  • Year and Date: 2011-12-02
• [Presentation] カニクイザルを用いた高濃度ベバシズマブ硝子体内投与における薬物動態の検討 (2011)
  • Author(s): 柿木雅志, 三宅太一郎, 澤田修, 澤田智子, 川村肇, 大路正人
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-13
• [Presentation] カニクイザルを用いたベバシズマブ硝子体内投与における必要最小量の検討 (2011)
  • Author(s): 三宅太一郎, 柿木雅志, 澤田修, 澤田智子, 川村肇, 大路正人
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-13
• [Presentation] The clearance of intravitreal bevacizumab in vitrectomized macaque eyes (2011)
  • Author(s): Kakinoki M, Miyake T, Sawada O, Sawada T, Kawamura H, Ohji M
  • Organizer: ARVO
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2011-05-05
• [Presentation] The minimum dose of intraviteal injection of bevacizumab effective against vascular endothelial growth factor in macaque eyes (2011)
  • Author(s): Miyake T, Kakinoki M, Sawada O, Sawada T, Kawamura H, Ohji M
  • Organizer: ARVO
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2011-05-05
• [Presentation] The clearance of intravitreal bevacizumab in vitrectomized macaque eyes (2011)
  • Author(s): Kakinoki M, Miyake T, Sawada O, Sawada T, Kawamura H, Ohji M.
  • Organizer: ARVO
  • Place of Presentation: Fort Lauderdale,米国
  • Year and Date: 2011-05-02
• [Presentation] The minimum dose of intravitreal injection of bevacizumab effective against vascular endothelial growth factor in macaque eyes (2011)
  • Author(s): Miyake T, Kakinoki M, Sawada O, Sawada T, Kawamura H, Ohji M.
  • Organizer: ARVO
  • Place of Presentation: Fort Lauderdale,米国
  • Year and Date: 2011-05-02
• [Presentation] カニクイザルを用いた無硝子体眼へのベバシズマブ投与におけるVEGF濃度の解析 (2010)
  • Author(s): 柿木雅志, 三宅太一郎, 澤田修, 澤田智子, 川村肇, 大路正人
  • Organizer: 第49回日本網膜硝子体学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-27
• [Presentation] Pharmacokinetics of bevacizumab and its Effect on vascular endothelial growth factor after intravitreal injection of bevacizumab in Macaque eyes (2010)
  • Author(s): Miyake T, Sawada O, Kakinoki M, Sawada T, Kawamura H, Ohji M
  • Organizer: ARVO
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2010-05-05
• [Presentation] Pharmacokinetics of bevacizumab and its Effect on vascular endothelial growth factor after intravitreal injection of bevacizumab in Macaque eyes (2010)
  • Author(s): Miyake T, Sawada O, Kakinoki M, Sawada T, Kawamura H, Ohji M.
  • Organizer: ARVO
  • Place of Presentation: Fort Lauderdale,米国
  • Year and Date: 2010-05-03
• [Presentation] カニクイザルを用いたベバシズマブ硝子体内投与における薬物動態の解析 (2010)
  • Author(s): 三宅太一郎、澤田修、柿木雅志、澤田智子、川村肇、大路正人
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-15