Pathophysiological analysis and development of therapeutic strategy for refractory corneal and external eye diseases

• Project/Area Number: 21592258
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Tottori University
• Principal Investigator: MIYAZAKI Dai 鳥取大学, 医学部附属病院, 講師 (30346358)
• Co-Investigator(Kenkyū-buntansha): INOUE Yoshitsugu 鳥取大学, 医学部, 教授 (10213183) ; BABA Takashi 鳥取大学, 医学部附属病院, 講師 (40304216) ; IKEDA Yoshifumi 鳥取大学, 医学部, 助教 (10444639)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 眼免疫学 / アレルギー性結膜炎 / 加齢黄斑変性症 / 角膜内皮 / 眼炎症 / 角膜 / ヘルペス

Research Abstract

To understand molecular pathology of ocular surface or corneal diseases, contribution of inflammatory or regenerative signals were integrally analyzed. Application of network model-based approach utilizing transcriptome or proteome of diseased tissues or models revealed intricate interactions of innate and acquired immune system in disease processes. The insight on integral regulatory pathways or contributing factors was further utilized to understand age related ocular diseases. Present findings may become basis to develop novel or more efficacious therapeutic strategy

Research Products

• [Journal Article] Associations of IL-23 with Polypoidal Choroidal Vasculopathy (2012)
  • Author(s): Sasaki S, Miyazaki D, et al
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 53 Issue: 7 Pages: 3424-30
  • DOI: 10.1167/iovs.11-7913
  • Peer Reviewed
• [Journal Article] Assessment of Real-Time Polymerase Chain Reaction Detection of Acanthamoeba and Prognosis Determinants of Acanthamoeba Keratitis (2012)
  • Author(s): Ikeda Y, Miyazaki D, et al
  • Journal Title: Ophthalmology
  • Peer Reviewed
• [Journal Article] Blood Level of Tacrolimus in Patients with Severe Allergic Conjunctivitis Treated by 0.1% Tacrolimus Ophthalmic Suspension (2012)
  • Author(s): Ebihara Y, Ohashi Y, Fujishima Y, Fukushima A, Nakagawa Y, Namba K, Okamoto S, Shoji J, Takamura E, Uchio, E, Miyazaki D
  • Journal Title: Allergology International Volume: 61 Issue: 2 Pages: 275-282
  • DOI: 10.2332/allergolint.11-OA-0349
  • NAID: 130004477125
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed
• [Journal Article] Associations of IL-23 with Polypoidal Choroidal Vasculopathy (2012)
  • Author(s): Sasaki S, Miyazaki D, Miyake K, Terasaka Y, Kaneda S, Ikeda Y, Funakoshi T, Baba T, Yamasaki A, Inoue Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: (In press)
  • Peer Reviewed
• [Journal Article] Herpes simplex virus type 1-induced transcriptional networks of corneal endothelial cells indicate antigen presentation function (2011)
  • Author(s): Miyazaki D, Haruki T, Takeda S, Sasaki S, Yakura K, Terasaka Y, Komatsu N, Yamagami S, Touge H, Touge C & Inoue Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52 Issue: 7 Pages: 4282-4293
  • DOI: 10.1167/iovs.10-6911
  • Peer Reviewed
• [Journal Article] Roles played by toll-like recetor-9 in corneal endothelial cells after herpes simplex virus type 1 infection (2011)
  • Author(s): Takeda S, Miyazaki D, Sasaki S, Yamamoto Y, Terasaka Y, Yakura K, Yamagami S, Ebihara N, & Inoue Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52 Issue: 9 Pages: 6729-6736
  • DOI: 10.1167/iovs.11-7805
  • Peer Reviewed
• [Journal Article] Multivariate analysis of inflammatory cytokines in eyes with branch retinal vein occlusion : Relationships to bevacizumab treatment (2011)
  • Author(s): Kaneda S, Miyazaki D, Sasaki S, Yakura K, Terasaka Y, Miyake K, Ikeda Y, Funakoshi T, Baba T, Yamasaki A, & Inoue Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52 Issue: 6 Pages: 2982-2988
  • DOI: 10.1167/iovs.10-6299
  • Peer Reviewed
• [Journal Article] Induction of IL-6 in transcriptional networks in corneal epithelial cells after herpes simplex virus type 1 infection (2010)
  • Author(s): Terasaka Y, Miyazaki D, Yakura K, Haruki T, & Inoue Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51 Issue: 5 Pages: 2441-2449
  • DOI: 10.1167/iovs.09-4624
  • Peer Reviewed
• [Journal Article] Role of periostin and interleukin-4 in recurrence of pterygia (2010)
  • Author(s): Kuo C-H, Miyazaki D, Yakura K, Araki-Sasaki K, & Inoue Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51 Issue: 1 Pages: 139-143
  • DOI: 10.1167/iovs.09-4022
  • Peer Reviewed
• [Journal Article] Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production (2010)
  • Author(s): Miyazaki D, Ishida W, Tominaga T, Sumi T, Fukushima A
  • Journal Title: Jpn J Ophthall Volume: 54(5) Issue: 5 Pages: 476-80
  • DOI: 10.1007/s10384-010-0837-6
  • NAID: 10027665935
  • Peer Reviewed
• [Journal Article] ゲノムからみたアレルギー疾患とアレルギー性結膜炎 (2010)
  • Author(s): 宮崎大
  • Journal Title: 日本眼科学会雑誌 Volume: 114巻9号 Pages: 783-790
  • NAID: 10029366767
  • Peer Reviewed
• [Journal Article] Induction of IL-6 in transcriptional networks in corneal epithelial cells after herpes simplex virus type 1 infection. (2010)
  • Author(s): Terasaka Y
  • Journal Title: Invest Ophthalmol Via Sci Volume: 51 Pages: 2441-9
  • Peer Reviewed
• [Journal Article] Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. (2010)
  • Author(s): Miyazaki D
  • Journal Title: Jpn J Ophthalmol Volume: 54 Pages: 476-80
  • NAID: 10027665935
  • Peer Reviewed
• [Journal Article] Blocking mast cell-mediated type 1 hypersensitivity in experimental allergic conjunctivitis by monocyte chemoattractant protein-1/CCR2 (2009)
  • Author(s): Tominaga T, Miyazaki D, Sasaki S, Mihara S, Komatsu N, Yakura K & Inoue Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 50 Issue: 11 Pages: 5181-5188
  • DOI: 10.1167/iovs.09-3637
  • Peer Reviewed
• [Journal Article] Regulatory function of CpG-activated B cells in late phase experimental allergic conjunctivitis (2009)
  • Author(s): Miyazaki D, Kuo C-H, Tominaga T, Inoue Y & Ono SJ
  • Journal Title: Invest Ophthalmol Volume: 50 Issue: 4 Pages: 1626-1635
  • DOI: 10.1167/iovs.08-2701
  • Peer Reviewed
• [Journal Article] Role of periostin and interleukin-4 in recurrence of pterygia (2009)
  • Author(s): Kuo CH
  • Journal Title: Invest Ophthalmol Vis Sci 51 Pages: 139-143
  • Peer Reviewed
• [Journal Article] Blocking mast cell-mediated type I hypersensitivity in experimental allergic conjunctivitis by monocyte chemoattractant protein-1/CCR2. (2009)
  • Author(s): Tominaga T
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 5181-5188
  • Peer Reviewed
• [Journal Article] Regulatory function of CpG-activated B cells in late-phase experimental allergic conjunctivitis (2009)
  • Author(s): Miyazaki D
  • Journal Title: Invest Ophthalmol Vis Sci 50 Pages: 1626-1635
  • Peer Reviewed
• [Journal Article] Comparison of efficacy of bromfenac sodium 0.1% ophthalmic solution and fluorometholone 0.02% ophthalmic suspension for the treatment of allergic conjunctivitis (2009)
  • Author(s): Fujishima H
  • Journal Title: J Ocul Pharmacol Ther 25 Pages: 265-270
  • Peer Reviewed
• [Journal Article] Multivariate analyses of inflammatory cytokines in eyes with branch retinal vein occlusion : relationships to bevacizumab treatment.
  • Author(s): Kaneda S
  • Journal Title: Invest Ophthalmol Vis Sci Volume: (In press)
  • Peer Reviewed
• [Journal Article] Induction of IL-6 in transcriptional networks in corneal epithelial cells after Herpes simplex virus type 1 infection
  • Author(s): Terasaka Y
  • Journal Title: Invest Ophthalmol Vis Sci (In press)
  • Peer Reviewed
• [Journal Article] Staphylococcus aureus enterotoxin B aggravates conjunctival early-phase reaction via augmentation of IgE production
  • Author(s): Miyazaki D
  • Journal Title: Japanese Journal of Ophthalmol (in press)
  • Peer Reviewed
• [Presentation] Indoleamine 2, 3-deoxygenaseによる角膜内皮の免疫制御作用 (2012)
  • Author(s): 春木智子、宮崎大, ほか
  • Organizer: 角膜カンファランス2012(第36回日本角膜学会総会・第28回日本角膜移植学会
  • Place of Presentation: ホテルニューオータニ(東京)
• [Presentation] マウスアレルギー性結膜炎モデル即時型過敏反応におけるIκB kinaseの役割 (2011)
  • Author(s): 佐々木慎一
  • Organizer: 角膜カンファランス
  • Place of Presentation: 品川プリンスホテル(東京)
  • Year and Date: 2011-02-18
• [Presentation] 炎症性側面からみた眼アレルギーと加齢性網膜疾患 (2011)
  • Author(s): 宮崎大
  • Organizer: 第61回日本アレルギー学会秋期学術大会シンポジウム
  • Place of Presentation: 東京
• [Presentation] Pharmacological inhibition of IκB kinase aggravates ocular allergic reactions (2010)
  • Author(s): Dai Miyazaki
  • Organizer: ARVO
  • Place of Presentation: Broward County Convention Center (Fort Lauderdale)
  • Year and Date: 2010-05-03
• [Presentation] Pharmacological inhibition of IκB kinase aggravates ocular allergic reactions Pharmacological inhibition of IκB kinase aggravates ocular allergic reactions (2010)
  • Author(s): Miyazaki D, Sasaki, S, Mihara S, Tominaga T, Yakura K, Inoue Y
• [Presentation] 結膜アレルギーにおけるNFkB経路の役割 (2009)
  • Author(s): 宮崎大
  • Organizer: 第63回臨床眼科学会オキュラーサーフェス研究会
  • Place of Presentation: 福岡
  • Year and Date: 2009-10-09
• [Presentation] 結膜アレルギーにおけるNFkB経路の役割 (2009)
  • Author(s): 宮崎大
  • Organizer: 第63回臨床眼科学会 オキュラーサーフェス研究会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-10-09
• [Presentation] ケモカインを標的にした肥満細胞制御とアレルギー性結膜炎の病態第63回臨床アレルギー研究会におけるNFkB経路の役割 (2009)
  • Author(s): 宮崎大
  • Organizer: 第63回臨床眼科学会オキュラーサーフェス研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-06-20
• [Presentation] アレルギー性結膜炎治療の新たな展開と免疫抑制剤 (2009)
  • Author(s): 宮崎大
  • Organizer: 第8回Symposium of Ocular Surface and Infection
  • Place of Presentation: 大阪
  • Year and Date: 2009-05-23
• [Presentation] The immunological mechanism on allergic ocular surface diseases (2009)
  • Author(s): Miyazaki D
  • Organizer: Asia Pacific Academy of Ophthalmology Meeting
  • Place of Presentation: Bali, Indonesia