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Effect of cholangitis after Kasai's portoenterostomy and prognostic fators on the molecular profiles of fecal bacterial flora in an infant with biliary atresia

Research Project

Project/Area Number 21592271
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatric surgery
Research InstitutionHokkaido University

Principal Investigator

OKADA Tadao  北海道大学, 北海道大学病院, 講師 (30344469)

Co-Investigator(Kenkyū-buntansha) SASAKI Fumiaki  北海道大学, 大学院・医学研究科, 教授 (40178661)
OZAKI Michitaka  北海道大学, 大学院・医学研究科, 特任教授 (80256510)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords迅速遺伝子解析 / 細菌プロファイル / T-RFLP法 / 16S-rRNA / 胆道閉鎖症 / 胆管炎 / 糞便 / バイオロジカル・マーカー / 細菌プロファイリング / バイオロジカルマーカー
Research Abstract

Molecular methods utilizing broad-range primers for 16S rDNA(ribosomal RNA gene) PCR and sequencing have been evaluated for their utility in diagnostic bacteriology. First, microbiota in fecal content from BA individual with recurrent cholangitis was compared using 16S rRNA gene libraries and terminal restriction fragment length polymorphism(T-RFLP). Second, microbiota in fecal content from choledochal cyst(CC) and biliary atresia(BA) individuals at the operation were compared using 16S rRNA gene libraries and T-RFLP As the results, any bile microbiota(bacterial DNA) was not detected using PCR in CC and BA patients. Bacterial DNA showed marked differences in the composition of fecal microbiota in a BA infant with recurrent cholangitis. Molecular analysis of colonic microbiota using 16S rRNA gene libraries and T-RFLP might be useful to detect BA cholangitis more earlier. Bacterial DNA showed marked differences in the composition of fecal microbiota in CC and BA infants. The microbiota consisted of microbial communities of Bifidobacterium, Lactobacillales, Bacteroides, Prevotella, Clostridium clusters IV, XI, and XVIII, and Clostridium subcluster XIVa. The Bifidobacterium, Bacteroides, and Clostridium clusters were detected predominatly in CC than BA group. Lactobacillales was most predominat group in BA feces. Molecular analysis of colonic microbiota using 16S rRNA gene libraries and T-RFLP might be useful to differentiate CC from BA.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (4 results)

All 2012 Other

All Presentation (3 results) Remarks (1 results)

  • [Presentation] Bacterial 16S rRNA genetic markers for fecal samples to differentiate choledochal cyst from biliary atresia2012

    • Author(s)
      T. Okada, S. Honda, H. Miyagi, A. Taketomi
    • Organizer
      4th Congress of European Academy of Pediatric Surgeons
    • Place of Presentation
      Istanbul
    • Related Report
      2011 Final Research Report
  • [Presentation] Molecular analysis of bile microbiota using PCR and sequencing of independent genetic targets in pediatric biliary diseases2012

    • Author(s)
      T. Okada, S. Honda, H. Miyagi, A. Taketomi
    • Organizer
      4th World Congress of Pediatric Gastroenterology, Hepatology and Nutrition
    • Place of Presentation
      Taipei
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of cholangitis after Kasai's portoenterostomy on the molecular profiles of fecal bacterial flora in an infant with biliary atresia2012

    • Author(s)
      T. Okada, S. Honda, H. Miyagi, A. Taketomi
    • Organizer
      4th World Congress of Pediatric Gastroenterology, Hepatology and Nutrition
    • Place of Presentation
      Taipei
    • Related Report
      2011 Final Research Report
  • [Remarks] 北海道大学病院自主臨床研究(自011-0017)

    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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