Molecular biological analysis by organ culture and application to fetal treatment using mice model with anorectal malformation

• Project/Area Number: 21592276
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Shiga University of Medical Science
• Principal Investigator: KUBOTA Yoshihiro 滋賀医科大学, 医学部, 助教 (30305601)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 直腸肛門奇形 / 器官培養 / 胎仔治療

Research Abstract

A single high dose of etretinate, a long-acting synthetic retinoid, induces ARMs in mice. Abnormal intrinsic innervation of mice embryos with anorectal malformations at later developmental stages was eliminated by molecular biological methods. Organ culture was quite difficult, but it will be possible to succeed it. It is necessary to continue to study it.

Research Products

• [Journal Article] Abnormal development of intrinsic innervation in murine embryos with anorectal malformations (2012)
  • Author(s): Kubota Y, Cho H, Umeda T, Abe H, Kurumi Y, Tani T.
  • Journal Title: Pediatric Surgery International Volume: 28(3) Pages: 295-298
  • Peer Reviewed
• [Journal Article] Abnormal development of intrinsic innervation in murine embryos with anorectal malformations (2012)
  • Author(s): Yoshihiro Kubota, Hirotomi Cho, Tomoko Umeda, Hajime Abe, Yoshimasa Kurumi, Toru Tani
  • Journal Title: Pediatric Surgery International Volume: 28 Pages: 295-298
  • Peer Reviewed
• [Presentation] Abnormal development of intrinsic innervation of murine embryos with anorectal malformation (2010)
  • Author(s): Kubota Y.
  • Organizer: Pacific Association of Pediatric Surgeons
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-05-26
• [Presentation] Abnormal development of intrinsic innervation of marine embryos with anorectal malformation (2010)
  • Author(s): Yoshihiro Kubota
  • Organizer: Pacific Association of Pediatric Surgeons
  • Place of Presentation: ポートピアホテル、神戸
  • Year and Date: 2010-05-26
• [Remarks]
  • URL: http://shiga-med.ac.jp/~hqsurge1/hqsurge1/staff.html