| Project/Area Number |
21592281
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUGITO Kiminobu 日本大学, 医学部, 助教 (10328750)
NAGASE Hiroki 千葉県がんセンター, 研究所, 所長 (90322073)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | MYCN遺伝子 / PIポリアミド / 発現抑制 / 小児外科 / 小児固形腫瘍 / 癌関連遺伝子 / 神経芽腫 / ピロールイミダゾールポリアミド |
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| Research Abstract |
Neuroblastoma(NB) is the most common pediatric solid tumor. NB has MYCN amplified is poor outcome and down regulation of MYCN expression causes proliferation down-regulated and differentiation in NB. We designed PI polyamide targeting for SP1 and E2F binding site, which are promoter region of MYCN gene. NB cell lines, such as CHP134 cells were cultured in the PI polyamide. NB cell lines were down-regulation of proliferation and of MYCN expression, compared with NB cell lines untreated with PI polyamide. We made xenograft nude mice by using CHP134 cells. In NB xeno-graft mice, was we administrated PI polyamide into the tumor. Then the tumors growth was down-regulated, compared with non-treated group. This PI polyamide could be one of the new therapeutic agents for MYCN gene amplified neuroblastoma.
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