Basic research about the development of the allogeneic reconstituted skin equivalent which induces local immunotolerance

Research Project

Project/Area Number	21592290
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Plastic surgery
Research Institution	Sapporo Medical University
Principal Investigator	MATSUMOTO Yoshitaka 札幌医科大学, 医学部, 助教 (70452977)
Co-Investigator(Kenkyū-buntansha)	YOTSUYANAGI Takatoshi 札幌医科大学, 医学部, 教授 (70250595) 江副京理札幌医科大学, 医学部, 講師 (20274938)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	組織培養 / 移植学 / 同種皮膚移植 / 免疫寛容 / サイトカイン / 再構築皮膚 / 3次元培養 / 組織工学 / 不死化株 / HPV E7
Research Abstract	Basic research was performed with inbred rat strain to develope the allogeneic reconstituted skin equivalent inducing local immunotolerance. Keratinocyte and fibroblast was derived from rat skin, and the keratinocyte was immortalized(AK-E7). The reconstituted skin equivalent, using AK-E7 with fibroblast, showed nearly normal histologic epidermal structure. The cultured supernatant of the fibroblast transfected with IL-10 cDNA(AFB-IL10) inhibited mixed lymphocyte reaction. As the reconstituted skin equivalent using AK-E7 and AFB-IL10 was grafted into autograft model, its partial take was recognized histologically.