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Establishment of new molecular therapy of keloid and elucidation of mechanism of development by IL-23/IL-17 pathway

Research Project

Project/Area Number 21592298
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionNippon Medical School

Principal Investigator

TOSA Mamiko  日本医科大学, 医学部, 助教 (30301568)

Co-Investigator(Kenkyū-buntansha) MOHAMMAD Ghazizadeh  日本医科大学, 老人病研究所, 准教授 (30190979)
MURAKAMI Masahiro  日本医科大学, 医学部, 准教授 (00239500)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsケロイド / IL-17 / IL-23 / ケロイドモデル / 培養細胞 / Th17
Research Abstract

We have reported that IL-6 mediated inflammation is a key player and may be considered as a common causative factor for development of keloid. IL-17, a recently discovered pro-inflammatory cytokine, is secreted by a distinct subtype of activated CD4 T-cells known as Th17.We first examined the distribution of Th17 cells in peripheral blood and keloid tissues obtained from the patients. There was an increased in Th17 cells as compared with normal individuals. The expression of IL-17 gene in keloid derived fibroblasts(KF) was higher than in normal dermis derived fibroblasts(NF). We measured mRNA expressions of two principal ECM molecules, COL1A2 and FN1, in NF cultures after addition of IL-17A peptide or in KF cultures after inhibition of IL-17 with anti-IL-17 antibody at various concentrations. Addition of IL-17A in NF culture medium significantly and dose-dependently enhanced COL1A2 and FN1 mRNA expressions, whereas inhibition of IL-17 by anti-IL-17 antibody in KF culture medium lowered COL1A2 and FN1 mRNA expressions. Our results indicate that IL-17 signaling may play an integral role in keloid pathogenesis and provide clues for development of IL-17 blocking strategies for therapy or prophylaxis of keloid.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (7 results)

All 2011 2010 2009

All Presentation (7 results)

  • [Presentation] ケロイドにおけるTh17細胞のIL-6シグナル促進効果の検討2011

    • Author(s)
      土佐眞美子、ガジザデ・モハマッド、村上正洋、百束比古
    • Organizer
      第20回日本形成外科基礎学術集会
    • Place of Presentation
      新宿
    • Year and Date
      2011-10-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 炎症的側面と腫瘍的側面を持つケロイドに対する基礎的アプローチ2011

    • Author(s)
      土佐眞美子
    • Organizer
      第20回日本形成外科学会基礎学術集会
    • Place of Presentation
      ハイアットリージェンシー東京
    • Year and Date
      2011-10-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional implication of the IL-6 signaing pathway in keloid pathogenesis2010

    • Author(s)
      Mamiko Tosa, Mohammad Ghazizadeh
    • Organizer
      International scar meeting in Tokyo
    • Place of Presentation
      東京
    • Year and Date
      2010-11-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] ケロイドにおけるTh17細胞のIL-6シグナル促進効果の検討2010

    • Author(s)
      土佐眞美子、モハマッド・ガジザデ
    • Organizer
      第19回日本形成外科学会基礎学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 炎症的側面と腫瘍的側面を持つケロイドに対する基礎的アプローチ2010

    • Author(s)
      土佐眞美子、ガジザデ・モハマッド、村上正洋、百束比古
    • Organizer
      第19回日本形成外科基礎学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] ケロイド患者におけるヘルパーT細胞サブタイプの解析2009

    • Author(s)
      土佐眞美子、ガジザデ・モハマッド、村上正洋、百束比古
    • Organizer
      第18回日本形成外科基礎学術集会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-01
    • Related Report
      2011 Final Research Report
  • [Presentation] ケロイド患者におけるヘルパーT細胞の分布解析2009

    • Author(s)
      土佐眞美子
    • Organizer
      第18回日本形成外科学会基礎学術集会
    • Place of Presentation
      東京都・都市センターホール
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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