Effect of local environment, fibrin, and basic FGF incorporation on a canine autologous model of bio-engineered cartilage tissue
| Project/Area Number |
21592300
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Plastic surgery
|
|---|
| Research Institution | Kinki University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ASAMURA Shinichi 近畿大学, 医学部, 准教授 (20340804)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
|
|---|
| Keywords | 生分解性高分子 / 自家移植 / 軟骨再生 / bFGF / 徐放システム / フィブリン / 生分解性ポリマー / 軟骨 / 組織工学 / ポリマー |
|---|
| Research Abstract |
We developed a technique to form a bioabsorbable synthetic polymer(poly glycolic acid ; PGA) combined with a natural polymer(fibrin) to serve as a scaffold to help retain seeded cells and improve the seeding efficiency of chondrocytes in an implantable construct. This approach was evaluated in a canine autologous implant model of bio-engineered cartilage. The implantation site(subcutaneous or intra-fascial) and the use of basic fibroblast growth factor(b-FGF) were also evaluated with this system. The intra-fascial implantation site yielded optimal results, especially when used in conjunction with fibrin and a b-FGF sustained-release system incorporated into the complex. A thicker, more sustained cartilagenous layer was formed, with a more vascularized outer fibrous supporting tissue layer. This combined approach of implant environment selection, natural polymer for cell retention, and growth factor supplementation offers a more optimized method for generating bio-engineered auricular cartilage.
|
Report
(4 results)
Research Products
(20 results)
-
-
-
-
-
-
-
-
[Journal Article]2009
Author(s)
楠原廣久
-
Journal Title
患者までとどいている再生誘導治療(株式会社メディカルドゥ)
Pages: 144-149
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
